Genomic Sequencing of Aged Nonhuman Primates at ONPRC

ONPRC 对老年非人类灵长类动物进行基因组测序

• 批准号: 10662148
• 负责人: Peter Gordon Barr-Gillespie
• 金额: $ 14.34万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-08 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10662148
• 关键词: Aging; Animals; Biomedical Research; Core Grant; DNA; DNA Sequence; DNA sequencing; Data; Databases; Disease; Genetic; Genetic Polymorphism; Genomics; Genotype; Individual; Infrastructure; Libraries; Link; Longevity; Macaca; Macaca mulatta; Maps; Oregon; Phenotype; Primates; Process; Protocols documentation; Research; Resources; Running; Sampling; Sequence Read Archive; Single Nucleotide Polymorphism; Technology; Time; United States National Institutes of Health; Variant; X Chromosome; Y Chromosome; aged; animal data; base; cost; formycin triphosphate; genetic pedigree; instrumentation; nonhuman primate; offspring; public database; reference genome; reproductive; sex; translational model

PROJECT SUMMARY The Oregon National Primate Research Center (ONPRC) is one of seven such National Primate Research Centers (NPRCs) that are sponsored by the National Institutes of Health. ONPRC is tasked to conduct scientific discovery in order to help the public live healthier, longer lives. Facilitating this process, support for ONPRC comes through the P51 Core Grant (P51-OD011092), which provides expertise, technology, and various Core- based resources. This infrastructure at ONPRC provides access to nonhuman primates (ca. 5,000 individuals), enabling a highly translational model for cutting-edge biomedical research. The genetics department at ONPRC is one of several specialized resources and they help facilitate and maintain the outbred resource locally and in coordination with the other NPRCs. Moreover, to better define individuals and their genotypic variation (for example, single nucleotide polymorphisms; SNP) the genetics staff has been performing DNAseq and compiling this information. Moreover, ONPRC is hosting similar information from other NPRCs and this data is available in an on-line format. However, due to cost and time-constraints, not to mention the large number of animals here and other NPRCs, the choice of animals to have their DNA sequenced is restrictive. Therefore, it is extremely helpful that the NIA is providing support via this project, to sequence the DNA from animals in the Primate Aging Study. This effort is interesting in other respects, such as an extended impact of having data from animals that may be well-represented in off-spring currently in the colony, which were produced over the reproductive lifespan of these individuals.

项目摘要 俄勒冈州国家灵长类动物研究中心（ONPRC）是七个国家灵长类动物研究中心之一 中心（NPRCs）由美国国立卫生研究院赞助。ONPRC的任务是进行科学研究， 为了帮助公众活得更健康，更长寿。为促进这一进程， 通过P51核心补助金（P51-OD 011092），提供专业知识，技术和各种核心， 基于资源。ONPRC的这一基础设施提供了接触非人类灵长类动物的机会（约100万美元）。5，000人）， 为尖端生物医学研究提供高度转化的模型。 ONPRC的遗传学部门是几个专业资源之一，他们帮助促进和维护 当地远系繁殖的资源，并与其他NPRC协调。此外，为了更好地定义个人 和它们的基因型变异（例如，单核苷酸多态性; SNP），遗传学工作人员已经 执行DNAseq并编译这些信息。此外，ONPRC还从其他国家接收类似信息。 这些数据以在线格式提供。然而，由于成本和时间的限制，更不用说 这里和其他NPRC的大量动物，选择动物进行DNA测序是 限制性的因此，NIA通过该项目提供支持是非常有帮助的， 灵长类衰老研究中动物的DNA。这一努力在其他方面也很有趣，例如 具有可能在殖民地中的后代中具有良好代表性的动物数据的影响， 在这些个体的生殖寿命期间产生的。

项目成果

Characterization of SALL2 Gene Isoforms and Targets Across Cell Types Reveals Highly Conserved Networks.

• DOI: 10.3389/fgene.2021.613808
• 发表时间: 2021
• 期刊: Frontiers in genetics
• 影响因子: 3.7
• 作者: Farkas C;Quiroz A;Alvarez C;Hermosilla V;Aylwin CF;Lomniczi A;Castro AF;Hepp MI;Pincheira R
• 通讯作者: Pincheira R

Brain xanthophyll content and exploratory gene expression analysis: subspecies differences in rhesus macaque.

• DOI: 10.1186/s12263-017-0557-3
• 发表时间: 2017
• 期刊: Genes & nutrition
• 影响因子: 3.5
• 作者: Mohn ES;Erdman JW Jr;Neuringer M;Kuchan MJ;Johnson EJ
• 通讯作者: Johnson EJ

Social hair pulling in captive rhesus macaques (Macaca mulatta).

• DOI: 10.1002/ajp.22720
• 发表时间: 2017-12
• 期刊: American journal of primatology
• 影响因子: 2.4
• 作者: Heagerty A;Wales RA;Prongay K;Gottlieb DH;Coleman K
• 通讯作者: Coleman K

Study of single and multidigit activation in monkey somatosensory cortex using voltage-sensitive dye imaging.

使用电压敏感染料成像研究猴子体感皮层的单指和多指激活

• DOI: 10.1117/1.nph.4.3.031219
• 发表时间: 2017-07
• 期刊: Neurophotonics
• 影响因子: 5.3
• 作者: Roe AW;Winberry JE;Friedman RM
• 通讯作者: Friedman RM

Prevention of tuberculosis in rhesus macaques by a cytomegalovirus-based vaccine.

• DOI: 10.1038/nm.4473
• 发表时间: 2018-03
• 期刊: Nature medicine
• 影响因子: 82.9
• 作者: Hansen SG;Zak DE;Xu G;Ford JC;Marshall EE;Malouli D;Gilbride RM;Hughes CM;Ventura AB;Ainslie E;Randall KT;Selseth AN;Rundstrom P;Herlache L;Lewis MS;Park H;Planer SL;Turner JM;Fischer M;Armstrong C;Zweig RC;Valvo J;Braun JM;Shankar S;Lu L;Sylwester AW;Legasse AW;Messerle M;Jarvis MA;Amon LM;Aderem A;Alter G;Laddy DJ;Stone M;Bonavia A;Evans TG;Axthelm MK;Früh K;Edlefsen PT;Picker LJ
• 通讯作者: Picker LJ