Endocrine disrupting activity associated with hydraulic fracturing

与水力压裂相关的内分泌干扰活动

• 批准号: 9198222
• 负责人: SUSAN C NAGEL
• 金额: $ 18.75万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9198222
• 关键词: Air; Analytical Chemistry; Androgen Receptor; Androgens; Area; Biological Assay; Carcinogens; Cells; Chemicals; Chemistry; Colorado; County; Endocrine; Endocrine Disruptors; Endocrine disruption; Endocrine system; Environment; Estrogen Antagonists; Estrogen Receptors; Estrogens; Exhibits; Exposure to; Failure; Fingerprint; Forensic Medicine; Fractionation; Fracture; Future; Health; Hormonal; Hormone Receptor; Hormones; Human; Individual; Isotopes; Liquid substance; Measures; Metabolism; Missouri; Natural Gas; Oils; Pathway interactions; Phase; Probability; Process; Regulation; Reporter Genes; Reporting; Reproduction; Reproductive system; Risk; Site; Solid; Source; Surface; Thyroid Gland; Water; androgenic; body system; contaminated drinking water; contaminated water; design; drinking water; estrogenic; experience; exposed human population; ground water; in vivo; innovation; operation; public health relevance; receptor; screening; tool; toxicant; wasting; water quality; water sampling

 DESCRIPTION (provided by applicant): Thousands of manmade chemicals have the potential to disrupt the endocrine system in humans. Termed endocrine disrupting chemicals (EDCs), they can cause negative effects on reproduction, metabolism and many other body systems. One potential source of human exposure to EDCs is through the use of hundreds of chemicals used in the process of hydraulic fracturing for natural gas and oil (fracking). More than 750 chemicals are reportedly used throughout this process, including more than 100 known or suspected endocrine disrupting chemicals. Spills and leaks of these chemicals and wastewater from the process are common. Importantly, the process was exempted from multiple federal regulatory acts in 2005 including the Safe Drinking Water Act, the Clean Water Act, and the Clean Air Act, which leaves regulation of this process largely with individual underfunded and understaffed states. Our overall hypothesis is that fracking and drilling operations contaminate surface and ground water with EDCs. We recently found an association between fracking and elevated endocrine disrupting activity in surface and ground water. Water from drilling dense areas had greater hormonal activity than drilling sparse reference sites in Garfield County. In the current proposal we aim to expand this analysis to 50 sites to determine if this association is broadly applicable and to identify chemicals responsible for the EDC activity. We will establish water quality measures and use forensic geochemical tools to determine if hydraulic fracturing fluids are present in the water samples. After solid-phase extracted, estrogen, androgen and thyroid receptor activity will be measured using reporter gene assays. We will use spatial analyses to determine if EDC activity is associated with proximity to drilling. Next, we will identfy endocrine active compounds by bioassay-guided fractionation followed by chemical profiling. It is essential to determine if fracking operations are contributing EDCs to water in order to determine the overall potential impacts of this process on human health. Completion of the proposed study will result in a more thorough understanding of the potential EDC contribution of fracking operations to surface and ground water in drilling-dense regions.



项目成果

Surface water and groundwater analysis using aryl hydrocarbon and endocrine receptor biological assays and liquid chromatography-high resolution mass spectrometry in Susquehanna County, PA.

在宾夕法尼亚州萨斯奎哈纳县使用芳基碳氢化合物和内分泌受体生物测定以及液相色谱-高分辨率质谱法进行地表水和地下水分析。

• DOI: 10.1039/c9em00112c
• 发表时间: 2019
• 期刊: Environmental science. Processes & impacts
• 作者: Bamberger,Michelle;Nell,Marika;Ahmed,AhmedH;Santoro,Renee;Ingraffea,AnthonyR;Kennedy,RanaF;Nagel,SusanC;Helbling,DamianE;Oswald,RobertE
• 通讯作者: Oswald,RobertE