APPLIED RESEARCH

应用研究

• 批准号: 9358156
• 负责人: CHRISTIAN R ABEE
• 金额: $ 7.24万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9358156
• 关键词: Adult; Alleles; Animal Model; Animal Testing; Animals; Applications Grants; Applied Research; Arteriviridae; Arterivirus; Behavioral; Betaretrovirus; Biological Assay; Bordetella; Breeding; Classification; Complex; Custom; Cytomegalovirus; Development; Ensure; Evaluation; Female; Foundations; Future; Generations; Genes; Genetic; Genetic Variation; Genome; Genomics; Genotype; Goals; Hair; Herpesviridae; Heterozygote; Infection; Infectious Agent; Length; MHC Class I Genes; Major Histocompatibility Complex; Major Histocompatibility Complex Gene; Maternal antibody; Messenger RNA; Nurseries; Papio; Pilot Projects; Primates; Quantitative Reverse Transcriptase PCR; Research; Research Personnel; Research Project Grants; Research Project Summaries; Resources; Retroviridae; Rhadinovirus; SIV; Sanitation; Scheme; Serologic tests; Simian Foamy Virus; Simian T-lymphotropic virus 1; Surrogate Mothers; Testing; Transcript; Vaccinated; Virus; animal breeding; animal colony; animal resource; diagnostic assay; genetic pedigree; genome sequencing; germ free condition; human disease; improved; male; molecular diagnostics; novel; novel virus; offspring; research study; viral transmission; whole genome

APPLIED RESEARCH – Project Summary The Specific Pathogen Free Baboon Research Resource (SPFBRR) is a scientifically unique colony of Old World research primates that are free of 19 adventitious infectious agents that are commonly found in conventional baboon species. The applied research that is proposed in this grant application has been developed to strengthen and improve the resource. The first two specific aims include the development and implementation of 7 molecular diagnostic assays for retroviruses and herpesviruses that are already on the bioexclusion list, and a novel arterivirus that has recently been identified in research baboons. Application of these assays will allow us to identify incipient infections in nursery-raised animals sooner, thus decreasing the attrition rate from the nursery. The next two projects involve genomic sequencing efforts to further define the animals within the SPFBRR and to improve the quality of research studies. The first of these genetics projects is to sequence the major histocompatibility complex (MHC) class I mRNA transcripts of all of the breeding animals in the SPFBRR to identify the complete repertoire of MHC class I genes. This information will allow us to perform MHC class I genotypes on all progeny animal in the colony to better inform future research studies. The second genetics project will be to perform whole genome sequencing (WGS) on 26 animals from the SPFBRR: the genomes of all 13 male breeders and one offspring from each of them will be sequenced. This information will allow us to calculate a 7 to 8 generation genomic pedigree analysis to better manage future colony breeding genetics, and it will also allow us to search for functionally significant genetic variations that could help to direct and inform future research projects. The final applied research project is a pilot study to use female retired breeder beagles as surrogates for nursery-raised baboons. The goal of this project is to provide some additional “maternal” contact to ensure behaviorally normal adult baboons for the SPF breeding colony.

应用研究-项目摘要 无特定病原体狒狒研究资源（SPFBRR）是一个科学上独特的老年人群体。 世界研究灵长类动物，没有19种常见于 传统的狒狒种类在这项拨款申请中提出的应用研究已经 开发，以加强和改善资源。前两个具体目标包括发展和 * 对已经列入《生物多样性公约》的逆转录病毒和疱疹病毒进行7次分子诊断化验 生物排斥列表，以及最近在研究狒狒中发现的一种新型动脉炎病毒。的应用 这些检测将使我们能够更快地识别托儿所饲养的动物中的初期感染，从而减少 幼儿园的损耗率接下来的两个项目涉及基因组测序工作，以进一步确定 动物在SPFBRR和提高研究的质量。第一个遗传学项目 是对所有的繁殖后代的主要组织相容性复合体（MHC）I类mRNA转录本进行测序， 在SPFBRR中的动物，以确定MHC I类基因的完整库。这些信息可以让我们 对殖民地中的所有子代动物进行MHC I类基因型分析，以更好地为未来的研究提供信息。 第二个遗传学项目将对26只动物进行全基因组测序（WGS）。 SPFBRR：将对所有13只雄性繁殖者和每只繁殖者的一只后代的基因组进行测序。这 这些信息将使我们能够计算7至8代基因组谱系分析，以更好地管理未来 群体繁殖遗传学，它也将使我们能够寻找功能上重要的遗传变异， 可以帮助指导和通知未来的研究项目。最后的应用研究项目是一个试点研究， 用退休的雌性比格犬代替托儿所饲养的狒狒。该项目的目标是 提供一些额外的“母亲”接触，以确保行为正常的成年狒狒进行SPF繁殖 殖民地