APPLIED RESEARCH

应用研究-项目摘要 无特定病原体浣熊研究资源(SPFBRR)是一个科学上独一无二的老年群体 世界研究灵长类动物，没有19种外来传染病病原体，通常在 传统的狒狒物种。在这项拨款申请中提出的应用研究已经被 为加强和改进资源而开发的。前两个具体目标包括发展和 对已存在的逆转录病毒和疱疹病毒实施7种分子诊断方法 生物排斥名单，以及最近在研究狒狒中发现的一种新的动脉病毒。应用程序 这些检测将使我们能够更早地识别托儿所饲养的动物的早期感染，从而减少 苗圃的流失率。接下来的两个项目涉及基因组测序工作，以进一步定义 在SPFBRR内的动物和提高研究的质量。这些遗传学项目中的第一个 是对所有育种品种的主要组织相容性复合体(MHC)I类mRNA转录本进行测序 SPFBRR中的动物，以确定MHC I类基因的完整谱系。这一信息将使我们 对该群体中的所有后代动物进行MHC I类基因分型，以更好地为未来的研究提供信息。 第二个遗传学项目将对来自世界各地的26只动物进行全基因组测序 SPFBRR：将对所有13只雄性繁殖者及其每一只后代的基因组进行测序。这 信息将使我们能够计算7到8代基因组谱系分析，以更好地管理未来 群体育种遗传学，它还将使我们能够搜索功能上显著的遗传变异， 可以帮助指导未来的研究项目并为其提供信息。最后的应用研究项目是一项初步研究，以 用退休的雌性种子犬作为苗圃饲养的狒狒的替代品。这个项目的目标是 提供一些额外的“母性”接触，以确保行为正常的成年狒狒进行SPF繁殖 殖民地。

APPLIED RESEARCH – Project Summary The Specific Pathogen Free Baboon Research Resource (SPFBRR) is a scientifically unique colony of Old World research primates that are free of 19 adventitious infectious agents that are commonly found in conventional baboon species. The applied research that is proposed in this grant application has been developed to strengthen and improve the resource. The first two specific aims include the development and implementation of 7 molecular diagnostic assays for retroviruses and herpesviruses that are already on the bioexclusion list, and a novel arterivirus that has recently been identified in research baboons. Application of these assays will allow us to identify incipient infections in nursery-raised animals sooner, thus decreasing the attrition rate from the nursery. The next two projects involve genomic sequencing efforts to further define the animals within the SPFBRR and to improve the quality of research studies. The first of these genetics projects is to sequence the major histocompatibility complex (MHC) class I mRNA transcripts of all of the breeding animals in the SPFBRR to identify the complete repertoire of MHC class I genes. This information will allow us to perform MHC class I genotypes on all progeny animal in the colony to better inform future research studies. The second genetics project will be to perform whole genome sequencing (WGS) on 26 animals from the SPFBRR: the genomes of all 13 male breeders and one offspring from each of them will be sequenced. This information will allow us to calculate a 7 to 8 generation genomic pedigree analysis to better manage future colony breeding genetics, and it will also allow us to search for functionally significant genetic variations that could help to direct and inform future research projects. The final applied research project is a pilot study to use female retired breeder beagles as surrogates for nursery-raised baboons. The goal of this project is to provide some additional “maternal” contact to ensure behaviorally normal adult baboons for the SPF breeding colony.