High content assays for cellular and synaptic phenotypes

细胞和突触表型的高内涵测定

• 批准号: 9313718
• 负责人: Shelley L Halpain
• 金额: $ 42.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9313718
• 关键词: Adopted; Antibodies; Astrocytes; Autistic Disorder; Axon; Behavioral; Bioinformatics; Biological Assay; Biological Neural Networks; Bipolar Disorder; Cell Line; Cell model; Cells; Cellular Assay; Collaborations; Communities; Computer software; Data Set; Defect; Dendrites; Development; Disease model; Filler; Fluorescence Microscopy; Genes; Glutamates; Human; Human Engineering; Image; Image Analysis; Impairment; Label; Laboratories; Maintenance; Membrane; Mental Health; Mental disorders; Methods; Morphogenesis; Morphology; Neurites; Neuroglia; Neurons; Pathway Analysis; Pattern; Phenotype; Protocols documentation; Reporter; Research Personnel; Running; Schizophrenia; Structure; Synapses; Technology; Validation; Viral; autism spectrum disorder; base; biosignature; cell type; cellular imaging; genome editing; imaging modality; induced pluripotent stem cell; neural circuit; novel; open source; postsynaptic; pre-clinical; presynaptic; scale up; screening; small molecule libraries; synaptogenesis; tool

Project Summary Proper neural networks rely on the neurons' ability to generate neurites (axons and dendrites) and form synaptic connections with appropriate partners. Impairments in neuronal morphology and synapse composition in specific subtypes of neurons have been described in various mental health conditions, ranging from schizophrenia and bipolar disorder to autism spectrum disorder (ASD). Such altered neuronal morphology and synapse assembly presumably underlie disrupted neural circuit function, and therefore disrupted behavioral patterns. The objective of Project 4 is to create a suite of cellular imaging-based assays in hIPSC-derived cultures of differentiated neurons. These assays are based on quantitative fluorescence microscopy, and will focus on specific aspects of neuritogenesis and synaptogenesis that are relevant to ASD. The assays will be compatible with high-content screening technology, and will be applied to engineered hIPSC ASD models.

项目总结