High content assays for cellular and synaptic phenotypes

项目摘要 正确的神经网络依赖于神经元产生轴突(轴突和树突)并形成 与适当伙伴的突触联系。神经元形态和突触组成的损害 在不同的精神健康状况下，已经描述了特定的神经元亚型，范围从 从精神分裂症和双相情感障碍到自闭症谱系障碍(ASD)。这种改变的神经元形态和 突触组装可能是神经回路功能紊乱的基础，因此也是行为紊乱的原因 模式。项目4的目标是创建一套基于细胞成像的分析方法 分化神经元的培养。这些分析是基于定量荧光显微镜，并将 重点关注与ASD相关的神经发生和突触发生的特定方面。化验结果将是 兼容高含量筛选技术，并将应用于设计的HiPSC ASD模型。

Project Summary Proper neural networks rely on the neurons' ability to generate neurites (axons and dendrites) and form synaptic connections with appropriate partners. Impairments in neuronal morphology and synapse composition in specific subtypes of neurons have been described in various mental health conditions, ranging from schizophrenia and bipolar disorder to autism spectrum disorder (ASD). Such altered neuronal morphology and synapse assembly presumably underlie disrupted neural circuit function, and therefore disrupted behavioral patterns. The objective of Project 4 is to create a suite of cellular imaging-based assays in hIPSC-derived cultures of differentiated neurons. These assays are based on quantitative fluorescence microscopy, and will focus on specific aspects of neuritogenesis and synaptogenesis that are relevant to ASD. The assays will be compatible with high-content screening technology, and will be applied to engineered hIPSC ASD models.