FASEB SRC on Dynamic DNA Structures

FASEB SRC 动态 DNA 结构

• 批准号: 9121299
• 负责人: SUE JINKS-ROBERTSON
• 金额: $ 0.5万
• 依托单位: FEDERATION OF AMER SOC FOR EXPER BIOLOGY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-06 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9121299
• 关键词: Address; Adopted; Aging; Area; B-DNA; Biology; Collaborations; DNA; DNA Sequence; DNA Structure; Development; Discipline; Disease; Employee Strikes; Environment; Fostering; Funding; G-Quartets; Genetic Processes; Genetic Recombination; Genetic Transcription; Genome; Genomic DNA; Goals; Hand; Health; Hereditary Disease; Human; Immune response; Immunoglobulin Class Switching; Inherited; Investigation; Left; Malignant Neoplasms; Molecular Conformation; Neurologic; Outcome; Participant; Pathologic Processes; Physiological Processes; Process; Property; Repetitive Sequence; Replication-Associated Process; Request for Proposals; Research; Research Personnel; Resolution; Rivers; Role; Science; Scientist; Structure; Therapeutic; Transcriptional Activation; Trinucleotide Repeats; abstracting; career development; genome integrity; human disease; meetings; novel; posters; preference; programs; public health relevance; summer research; symposium

 DESCRIPTION (provided by applicant): This proposal requests partial support for the FASEB Science Research Conference on Dynamic DNA Structures in Biology, which will be held July 10-15, 2016, in Saxtons River, VT. For decades after its discovery, DNA was believed to be a rigid, right-handed double helix. It is now appreciated, however, that DNA is highly dynamic molecule and can assume an enormous variety of conformations such as cruciforms, left-handed helixes, three- and four-stranded helixes, and slip-stranded configurations. Repetitive DNA sequences, which are overrepresented in genomic DNA, are particularly prone to such structural transitions. The transient denaturation of the double helix that occurs during most DNA transactions (transcription, replication and recombination) also promotes dynamic transitions in DNA structure. Studies conducted in many labs worldwide have confirmed that structure-prone DNA sequences are central to the normal functioning of the genome. Transitions in DNA structure, for example, are important for regulating the activation of transcription and the specialized recombination responsible for immunoglobulin class switching. Unscheduled transitions, however, can have pathological consequences. Of particular significance, the expansion of structure-prone repeats underlies more than thirty hereditary neurological and developmental diseases in humans. Furthermore, dynamic DNA structures are associated with genome rearrangements observed in many human cancers. The long-term objective of this Conference is to enhance our understanding of how dynamic DNA structures form, how they are resolved, how they contribute to normal genetic processes and how they promote pathological outcomes such as genetic disease, cancer and aging. The specific aims are to explore current understanding of DNA structural transitions, to identify new avenues of investigation, to define novel mechanisms that promote formation and resolution of dynamic DNA structures, to stimulate collaborations, and to foster the long-term development of this important area by promoting participation of junior scientists. To that end, we will convene ~100 participants for five intense and highly interactive days of science. The program will include an opening keynote address, eight morning/evening scientific sessions and informal afternoon poster sessions. In addition to invited speakers, each scientific session will include four short talks chosen from submitted abstracts, with preference given to trainees and junior investigators. The significance and strength of this Conference is that it uniquely brings together investigators with a common focus on dynamic DNA transitions, but who have highly varied backgrounds, expertise and experimental approaches.

 描述（由申请人提供）：本提案要求对FASEB生物学动态DNA结构科学研究会议的部分支持，该会议将于2016年7月10日至15日在佛蒙特州萨克顿河举行。在DNA被发现后的几十年里，人们一直认为它是一种刚性的右旋双螺旋结构。然而，现在认识到，DNA是高度动态的分子，并且可以呈现各种各样的构象，例如十字形、左手螺旋、三链和四链螺旋以及滑动链构型。在基因组DNA中过度表达的重复DNA序列特别容易发生这种结构转变。在大多数DNA处理（转录、复制和重组）过程中发生的双螺旋的瞬时变性也促进了DNA结构的动态转变。在世界各地的许多实验室进行的研究已经证实，结构倾向的DNA序列是基因组正常功能的核心。例如，DNA结构的转变对于调节转录的激活和负责免疫球蛋白类别转换的专门重组是重要的。然而，计划外的转变可能会产生病理后果。特别重要的是，结构倾向重复序列的扩展是人类30多种遗传性神经和发育疾病的基础。此外，动态DNA结构与在许多人类癌症中观察到的基因组重排相关。本次会议的长期目标是加强我们对动态DNA结构如何形成、如何分解、如何促进正常遗传过程以及如何促进遗传疾病、癌症和衰老等病理结果的理解。具体目标是探索目前对DNA结构转变的理解，确定新的研究途径，确定促进动态DNA结构形成和解决的新机制，促进合作，并通过促进年轻科学家的参与促进这一重要领域的长期发展。为此，我们将召集约100名与会者参加五天紧张和高度互动的科学活动。该计划将包括一个开幕式主题演讲，八个上午/晚上的科学会议和非正式的下午海报会议。除了特邀演讲者外，每次科学会议还将包括从提交的摘要中选出的四个简短演讲，优先考虑受训人员和初级研究人员。本会议的重要性和力量在于，它独特地汇集了 研究人员共同关注动态DNA转换，但他们的背景，专业知识和实验方法差异很大。