Structural Biology Shared Service

结构生物学共享服务

• 批准号: 9750150
• 负责人: DAVID S WEBER
• 金额: $ 7.3万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9750150
• 关键词: Address; Americas; Award; Baltimore; Biological; Biometry; Cancer Center; Cancer Center Support Grant; Cigarette; Computer Assisted; Consultations; County; Crystallization; Data; Data Collection; Deposition; Development; Doctor of Philosophy; Drug Design; Enzymes; Flow Cytometry; Funding; Genomics; Grant; Hormone Responsive; Human; Image; Institutes; Institution; Investigational Therapies; Journals; Laboratories; Malignant Neoplasms; Maryland; Medical; Medical center; Methodology; Methods; Mission; Molecular; Molecular Biology; Molecular Mechanisms of Action; Molecular Probes; Molecular Structure; Nature; Nuclear Magnetic Resonance; Pathology; Peer Review; Pharmaceutical Chemistry; Pharmacy Schools; Population Sciences; Preparation; Protein Databases; Proteins; Public Health Schools; Publications; Publishing; Reader; Research; Research Personnel; Resolution; Resources; Robotics; Roentgen Rays; Sampling; School Dentistry; Services; Structure; System; Therapeutic; Training; Translational Research; United States; United States National Academy of Sciences; United States National Institutes of Health; Universities; X ray diffraction analysis; X-Ray Crystallography; acronyms; biobank; collaborative environment; college; computerized data processing; data warehouse; design; drug candidate; education resources; high throughput screening; instrument; instrumentation; investigator training; medical schools; member; neoplasm immunotherapy; novel; pre-clinical; programs; small molecule inhibitor; small molecule libraries; structural biology; training opportunity; tumor immunology; virology

6.0 Abstract: Structural Biology Shared Service The Structural Biology Shared Service (SBSS) provides investigators with the facilities and expertise to collect and interpret molecular structural information (nuclear magnetic resonance [NMR], X-ray crystallography) to understand the molecular mechanism(s) of action of proteins and enzymes that contribute to cancer. The SBSS also facilitates identification of molecular agents (e.g., candidate drugs, molecular probes) that can be used to study the function of cancer targets and may have therapeutic potential. To achieve this mission, the SBSS implements a structural biology approach. This includes isolation and characterization of samples for NMR and X ray and application of state-of-the-art data collection methods in a collaborative environment that facilitates access by UMGCC investigators. In 2014, 36 UMGCC investigators used the SBSS. These investigators deposited 84 high-resolution structures into the protein database in addition to publishing in high- impact journals. The efforts of the SBSS have facilitated development of several novel, cancer target–specific small-molecule inhibitors. Interactions between the SBSS and investigators from multiple UMGCC programs have facilitated the integration of structural biology into the translational research efforts of UMGCC. Also of significance, the SBSS installed and commissioned a new 950 MHz NMR spectrometer purchased through an $8 million NIH Shared Instrumentation Grant. This is the only such instrument available in an academic institution in the United States and represents the largest Shared Instrumentation Grant awarded by NIH. The SBSS has also expanded and upgraded X-ray diffraction and robotic crystallization resources to enhance the use of these resources. In 2014, the SBSS supported 36 Cancer Center members spanning all 5 research programs (14 percent of all UMGCC members); 53 percent of UMGCC users have peer-reviewed funding. The SBSS supports many cancer-related publications annually, many in high-impact journals including Proceedings of the National Academy of Sciences of the United States of America, Journal of Medicinal Chemistry, and Nature Structural & Molecular Biology.

6.0摘要：结构生物学共享服务 结构生物学共享服务(SBSS)为研究人员提供了收集 并将分子结构信息(核磁共振、X射线结晶学)解释为 了解导致癌症的蛋白质和酶的分子机制(S)。这个 SBSS还有助于识别分子试剂(例如，候选药物、分子探针)，这些分子试剂可以 用于研究癌症靶点的功能，并可能具有治疗潜力。为了完成这一使命， SBSS实施了一种结构生物学方法。这包括样品的分离和表征 核磁共振和X射线以及在协作环境中应用最先进的数据收集方法 方便UMGCC调查人员进入。2014年，36名UMGCC调查人员使用了SBSS。这些 研究人员将84个高分辨率结构存入蛋白质数据库，并在高分辨率数据库中发表 影响日志。SBSS努力促进了几种新的、针对癌症靶点的开发 小分子抑制剂。SBSS与来自多个UMGCC项目的调查人员的互动 促进了将结构生物学纳入UMGCC的翻译研究工作。也可以是 重要的是，SBSS安装并调试了一台新的950 MHz核磁共振波谱仪，通过 800万美元的NIH共享仪器拨款。这是学术界唯一可用的此类工具。 是美国最大的共享仪器基金，由美国国立卫生研究院授予。这个 SBSS还扩展和升级了X射线衍射和机器人结晶资源，以增强 这些资源的使用。 2014年，SBSS支持了36名癌症中心成员，涵盖了所有5个研究项目(占所有研究项目的14% UMGCC成员)；53%的UMGCC用户拥有同行审查的资金。SBSS支持许多 每年都会发表与癌症有关的文章，其中许多发表在影响力很大的期刊上，包括《国家期刊》 美国科学院，《药物化学与自然结构与科学》杂志 分子生物学。