Mouse Microsurgery Core

核心B--摘要/摘要 小鼠肝脏显微外科核心(核心B)将负责进行肝脏移植手术 在这项PPG倡议中，对小鼠进行了程序和移植后护理。原位肝移植 小鼠的手术在技术上要求很高，保持手术的技术质量和 稳定性将是实验成功的关键。因此，核心B将提供所有手术援助以 肝热缺血和体外肝冷缺血联合肝移植、AS的研究进展 在项目I和项目II中概述。核心还将协调项目之间的实验，以最大限度地 使用来自移植的样本，并最大限度地减少移植手术的动物数量。此外，额外的 移植的样本将作为档案储存和维护。如果项目需要额外的手术 除了手术，核心B还将在提案中为其他外科需求提供支持。实验室里有 十年前建立了延长肝脏冷藏和原位肝移植的小鼠模型，以进行多方面的研究 器官移植中的先天免疫反应和获得性免疫反应。丰富的经验， 积累的数据和可用的模型将使我们能够自信地在 这个项目。核心B的具体目标如下： 目的1.提供高质量和一致性的小鼠原位肝移植和局部肝脏IRI手术及移植后护理。 核心将负责进行所有显微外科原位肝移植/非移植程序和术后- 项目1和2中的小鼠移植护理。这些程序在技术上要求很高，核心将 在进行实验时确保一致和可靠的手术结果，如本PPG所建议的那样。 目标2.协调不同项目之间的实验，最大限度地利用来自肝脏移植和 收件人。核心将协调项目I和项目II之间的实验，以最大限度地利用样本 从移植的动物中分离出来，从而最大限度地减少手术动物的数量和成本。 目标3.存储额外的样品并维护档案。核心将存储和记录多余的组织样本 (肝移植、宿主组织、血清)，并将其作为档案保存，以备将来使用。 目的4.为其他外科需求提供支持。核心B将为其他外科手术提供支持 (例如，门静脉注射；静脉注射。输液)，如果需要的话。

CORE B - SUMMARY/ABSTRACT The Mouse Liver Microsurgery Core (Core B) will be responsible for conducting liver transplant surgery procedures and post-transplant care in mice in this PPG initiative. The orthotopic liver transplantation (OLT) procedures in mice are technically highly demanding, and the maintenance of surgical technical quality and stability will be the key for the experimental success. Core B therefore will provide all the surgical assistance to the investigators in conducting liver warm ischemia as well as ex vivo liver cold ischemia combined with OLT, as outlined in Project I and Project II. The core will also coordinate experiments among the projects to maximize the use of samples from transplants and minimize the number of animals for transplant surgery. In addition, extra samples from transplants will be stored and maintained as the archive. If the Project requires additional surgical procedures, Core B will also provide support for other surgical needs in the proposals. The laboratory has developed the mouse model of extended hepatic cold storage and OLT a decade ago to study various aspects of innate and adaptive immune responses in organ transplantation. Significant amounts of experience, accumulated data, and available models will enable us to confidently conduct the complicated experiments in this Project. The specific aims of Core B are as follows: Aim 1. To provide OLT and local liver IRI surgery and post-transplant care in mice with quality and consistency. The core will be responsible for conducting all the microsurgical OLT/non-transplant procedures and post- transplant care in mice in Project 1 and 2. These procedures are technically demanding, and the Core will assure consistent and reliable surgical outcomes in conducting experiments, as proposed in this PPG. Aim 2. To coordinate experiments among projects and maximize the use of samples from liver transplants and recipients. The Core will coordinate experiments between Project I and Project II to maximize the use of samples from transplanted animals and thus to minimize the numbers and costs of animals for surgeries. Aim 3. To store extra samples and maintain the archive. The Core will store and record surplus tissue samples (liver grafts, host tissues, serum) and maintain them as an archive for potential future use. Aim 4. To provide support for other surgical needs. Core B will provide support for additional surgical procedures (e.g., portal vein injections; i.v. infusions), if needed.