Mouse Microsurgery Core

小鼠显微外科核心

• 批准号: 9750611
• 负责人: Haofeng Ji
• 金额: $ 16.81万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9750611
• 关键词: Address; Allogenic; Animals; Archives; Area; Caring; Clinical; Clinical Research; Cryopreservation; Data; Functional disorder; Future; Glean; Goals; Head; Hepatic; Hepatocellular Damage; Homeostasis; Human; Immune; Immune response; Immunity; Impairment; In Situ; Infusion procedures; Injections; Ischemia; Laboratories; Life; Liver; Maintenance; Mediating; Microscope; Microsurgery; Modeling; Mus; Operative Surgical Procedures; Organ Transplantation; Outcome; Performance; Portal vein structure; Procedures; Process; Protocols documentation; Regulation; Reperfusion Injury; Reperfusion Therapy; Research; Research Personnel; Role; Sample Size; Sampling; Serum; Signal Pathway; Stress; T-Lymphocyte; TLR4 gene; Technical Expertise; Testing; Therapeutic Intervention; Tissue Sample; Tissues; Training; Transgenic Organisms; Transplant Recipients; Transplantation; Transplantation Surgery; Warm Ischemia; Work; adaptive immune response; bench to bedside; cost; experience; experimental study; immune activation; immunoregulation; improved; innovation; insight; instrument; liver transplantation; macrophage; mouse model; novel; post-transplant; prevent; professor; success; surgery outcome; targeted treatment; transplant model

CORE B - SUMMARY/ABSTRACT The Mouse Liver Microsurgery Core (Core B) will be responsible for conducting liver transplant surgery procedures and post-transplant care in mice in this PPG initiative. The orthotopic liver transplantation (OLT) procedures in mice are technically highly demanding, and the maintenance of surgical technical quality and stability will be the key for the experimental success. Core B therefore will provide all the surgical assistance to the investigators in conducting liver warm ischemia as well as ex vivo liver cold ischemia combined with OLT, as outlined in Project I and Project II. The core will also coordinate experiments among the projects to maximize the use of samples from transplants and minimize the number of animals for transplant surgery. In addition, extra samples from transplants will be stored and maintained as the archive. If the Project requires additional surgical procedures, Core B will also provide support for other surgical needs in the proposals. The laboratory has developed the mouse model of extended hepatic cold storage and OLT a decade ago to study various aspects of innate and adaptive immune responses in organ transplantation. Significant amounts of experience, accumulated data, and available models will enable us to confidently conduct the complicated experiments in this Project. The specific aims of Core B are as follows: Aim 1. To provide OLT and local liver IRI surgery and post-transplant care in mice with quality and consistency. The core will be responsible for conducting all the microsurgical OLT/non-transplant procedures and post- transplant care in mice in Project 1 and 2. These procedures are technically demanding, and the Core will assure consistent and reliable surgical outcomes in conducting experiments, as proposed in this PPG. Aim 2. To coordinate experiments among projects and maximize the use of samples from liver transplants and recipients. The Core will coordinate experiments between Project I and Project II to maximize the use of samples from transplanted animals and thus to minimize the numbers and costs of animals for surgeries. Aim 3. To store extra samples and maintain the archive. The Core will store and record surplus tissue samples (liver grafts, host tissues, serum) and maintain them as an archive for potential future use. Aim 4. To provide support for other surgical needs. Core B will provide support for additional surgical procedures (e.g., portal vein injections; i.v. infusions), if needed.

核心B -总结/摘要 小鼠肝脏显微外科核心（核心B）将负责进行肝脏移植手术 程序和移植后护理的小鼠在这个PPG倡议。原位肝移植（奥尔特） 小鼠手术在技术上要求很高，维持手术技术质量和 稳定性将是实验成功的关键。因此，核心B将提供所有手术辅助， 研究者在进行肝脏热缺血以及离体肝脏冷缺血联合奥尔特中， 在项目I和项目II中概述。核心还将协调项目之间的实验，以最大限度地提高 使用来自移植的样本，并最大限度地减少移植手术的动物数量。此外，额外 移植的样本将作为档案储存和维护。如果项目需要额外的手术 核心B还将为提案中的其他手术需求提供支持。实验室已 十年前，我开发了延长肝脏冷藏和奥尔特的小鼠模型，以研究各个方面 先天性和适应性免疫反应在器官移植中的作用。丰富的经验， 积累的数据和可用的模型将使我们能够自信地进行复杂的实验， 这个项目核心B的具体目标如下： 目标1。为小鼠提供高质量和一致性的奥尔特和局部肝脏IRI手术和移植后护理。 核心将负责进行所有的显微外科奥尔特/非移植程序和术后 在项目1和项目2中的小鼠移植护理。这些程序在技术上要求很高，核心将 如本PPG所述，在进行实验时确保一致和可靠的手术结果。 目标二。协调各项目之间的实验，最大限度地利用肝脏移植样本， 受惠人士核心将协调项目I和项目II之间的实验，以最大限度地利用样本 从而最大限度地减少手术动物的数量和成本。 目标3。储存额外样本并维护存档。核心将储存并记录剩余组织样本 （肝移植物、宿主组织、血清），并将其作为档案保存，以备将来使用。 目标4。为其他手术需求提供支持。核心B将为额外的外科手术提供支持 (e.g.,门静脉注射;静脉内输注）。