Mouse Microsurgery Core

小鼠显微外科核心

• 批准号: 9975695
• 负责人: Haofeng Ji
• 金额: $ 17.58万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9975695
• 关键词: Address; Allogenic; Animals; Archives; Area; Caring; Clinical; Clinical Research; Cryopreservation; Data; Functional disorder; Future; Glean; Goals; Head; Hepatic; Hepatocellular Damage; Homeostasis; Human; Immune; Immune response; Immunity; Impairment; In Situ; Infusion procedures; Injections; Ischemia; Laboratories; Life; Liver; Maintenance; Mediating; Microscope; Microsurgery; Modeling; Mus; Operative Surgical Procedures; Organ Transplantation; Outcome; Performance; Portal vein structure; Procedures; Process; Protocols documentation; Regulation; Reperfusion Injury; Reperfusion Therapy; Research; Research Personnel; Role; Sample Size; Sampling; Serum; Signal Pathway; Stress; T-Lymphocyte; TLR4 gene; Technical Expertise; Testing; Therapeutic Intervention; Tissue Sample; Tissues; Training; Transgenic Organisms; Transplant Recipients; Transplantation; Transplantation Surgery; Warm Ischemia; Work; adaptive immune response; bench to bedside; cost; experience; experimental study; immune activation; immunoregulation; improved; innovation; insight; instrument; liver transplantation; macrophage; mouse model; novel; post-transplant; prevent; professor; success; surgery outcome; targeted treatment; transplant model

CORE B - SUMMARY/ABSTRACT The Mouse Liver Microsurgery Core (Core B) will be responsible for conducting liver transplant surgery procedures and post-transplant care in mice in this PPG initiative. The orthotopic liver transplantation (OLT) procedures in mice are technically highly demanding, and the maintenance of surgical technical quality and stability will be the key for the experimental success. Core B therefore will provide all the surgical assistance to the investigators in conducting liver warm ischemia as well as ex vivo liver cold ischemia combined with OLT, as outlined in Project I and Project II. The core will also coordinate experiments among the projects to maximize the use of samples from transplants and minimize the number of animals for transplant surgery. In addition, extra samples from transplants will be stored and maintained as the archive. If the Project requires additional surgical procedures, Core B will also provide support for other surgical needs in the proposals. The laboratory has developed the mouse model of extended hepatic cold storage and OLT a decade ago to study various aspects of innate and adaptive immune responses in organ transplantation. Significant amounts of experience, accumulated data, and available models will enable us to confidently conduct the complicated experiments in this Project. The specific aims of Core B are as follows: Aim 1. To provide OLT and local liver IRI surgery and post-transplant care in mice with quality and consistency. The core will be responsible for conducting all the microsurgical OLT/non-transplant procedures and post- transplant care in mice in Project 1 and 2. These procedures are technically demanding, and the Core will assure consistent and reliable surgical outcomes in conducting experiments, as proposed in this PPG. Aim 2. To coordinate experiments among projects and maximize the use of samples from liver transplants and recipients. The Core will coordinate experiments between Project I and Project II to maximize the use of samples from transplanted animals and thus to minimize the numbers and costs of animals for surgeries. Aim 3. To store extra samples and maintain the archive. The Core will store and record surplus tissue samples (liver grafts, host tissues, serum) and maintain them as an archive for potential future use. Aim 4. To provide support for other surgical needs. Core B will provide support for additional surgical procedures (e.g., portal vein injections; i.v. infusions), if needed.

核心B-摘要/摘要 小鼠肝显微外科手术核心（核心B）将负责进行肝移植手术 该PPG倡议中小鼠的过程和移植后护理。原位肝移植（OLT） 从技术上讲，小鼠的程序高度要求，维护手术技术质量和 稳定性将是实验成功的关键。因此，核心B将提供所有手术帮助 进行肝脏温暖缺血以及离体肝冷缺血与OLT相结合的研究者 在项目I和II项目中概述。核心还将协调项目之间的实验，以最大化 使用来自移植的样品，并最大程度地减少动物的移植手术数量。另外，额外 来自移植的样品将作为存档存储和维护。如果该项目需要其他手术 程序，核心B还将为提案中的其他外科手术需求提供支持。实验室有 十年前，开发了扩展的肝冷存储和OLT的鼠标模型，以研究各个方面 器官移植中的先天和适应性免疫反应。丰富的经验， 累积数据，可用模型将使我们能够自信地进行复杂的实验 这个项目。核心B的具体目的如下： 目标1。在小鼠中提供质量和一致性，在小鼠中提供OLT和局部肝脏IRI手术和移植后护理。 核心将负责执行所有显微外科/非移植程序和后 项目1和2中的小鼠的移植护理。这些过程在技术上是要求的，核心将会 如本PPG所建议的那样，确保进行实验的一致和可靠的手术结果。 目标2。协调项目之间的实验，并最大程度地使用肝移植中的样品 收件人。核心将协调项目I和项目II之间的实验，以最大化样品的使用 从移植动物，从而最大程度地减少动物的手术数量和成本。 目标3。存储额外的样品并维护档案。核心将存储和记录剩余的组织样品 （肝移植，宿主组织，血清）并将其保持为潜在的未来使用的档案。 目标4。为其他手术需求提供支持。核心将为其他手术程序提供支持 （例如，门静脉注射；如果需要）。