Core B: Immune Assessment

核心 B：免疫评估

• 批准号: 9751750
• 负责人: Maura Rossetti
• 金额: $ 7.81万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9751750
• 关键词: Address; Area; Biological Assay; Biopsy; Blood; Cell physiology; Clinical; Clinical Trials; Collaborations; Color; Communication; Computer software; Coupled; Cytomegalovirus; Cytomegalovirus Infections; Data; Data Analyses; Data Reporting; Databases; Education; Ensure; Equipment and supply inventories; Flow Cytometry; GEM gene; Goals; Health Insurance Portability and Accountability Act; Human Resources; Immune; Immune response; Immunoassay; Immunophenotyping; Informatics; Kidney Transplantation; Knowledge; Laboratories; Leadership; Liquid substance; Measures; Outcome; Performance; Periodicity; Peripheral Blood Mononuclear Cell; Plasma; Procedures; Protocols documentation; Reagent; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Serum; Specimen; Standardization; Systems Biology; Technical Expertise; Technology; Technology Assessment; Testing; Tissues; Training; Transplant Recipients; Urine; Viremia; Work; adaptive immune response; base; biobank; clinical phenotype; cost; data management; data sharing; design; electronic data; experimental study; heart allograft; immunological diversity; innovation; kidney allograft; lung allograft; meetings; new technology; programs; reagent standard; repository; synergism

CORE B: Immune Assessment SUMMARY/ABSTRACT The Immune Assessment Core B is essential to the research goals of the Project PIs collaborating in this HIPC Consortium. The proposed research aims to characterize the innate and adaptive immune response to CMV in recipients of heart, lung and renal allografts. Core B will provide skilled technical and scientific expertise to enable the PIs to achieve their research goals through the following specific Aims. Specific Aim 1. To provide professional expertise in the design and implementation of assays to measure immune cell function. The Core has validated standardized 14-color flow cytometry, multiplex immunoassays, and KIR typing assays to support the research of Projects 1 and 2. The Core will perform the assays and interpret the data. The IAC will ensure consistent and reliable intra- and inter-assay performance using standardized protocols, reagents and data interpretation. The core will coordinate experiments among the projects with the goal of maximizing testing uniformity and cost reduction. Specific Aim 2. To provide standardized sample processing, maintain a centralized biorepository of tissue, serum, plasma, peripheral blood mononuclear cells and urine, and enable efficient sample and data sharing. Processing and storage of graft biopsies, PBMCs, serum/plasma will be performed under standardized operating procedures honed by supporting clinical trials to be HIPAA compliant. The BTM database will be used to house the sample inventories at both UCLA and UCSF and associated data and made accessible to all PIs and staff. The Idea Elan core laboratory management software will be used to facilitate work flow and electronic data reporting to Data Management and Analysis Core B. Specific Aim 3. To provide education of PIs and staff in sample management, immune assessment technologies and data analysis/interpretation, and to disseminate the new knowledge in the HIPC Consortium. The Core will educate the investigators about technologies, their applications and limitations, and will promote communication and collaboration among projects and cores.

核心B：免疫评估 摘要/摘要 免疫评估核心B对于在本次重债穷国中合作的PIS项目的研究目标至关重要 财团。本研究旨在研究中国人对巨细胞病毒的天然免疫反应和获得性免疫反应。 心脏、肺和肾移植的接受者。核心B将提供熟练的技术和科学专门知识 使私人投资机构能够通过以下具体目标实现其研究目标。具体目标1.提供 具有设计和实施检测免疫细胞功能的专业知识。 Core已经验证了标准化的14色流式细胞术、多重免疫分析和KIR分型分析 支持项目1和项目2的研究。核心将执行分析并解释数据。IAC 将使用标准化的协议、试剂确保一致和可靠的检测内和检测间性能 和数据解释。核心将协调项目之间的实验，目标是最大化 测试一致性和成本降低。具体目标2.为了提供标准化的样品处理， 维护组织、血清、血浆、外周血单核细胞和 尿液，并实现高效的样本和数据共享。移植物活检、PBMCs、 血清/血浆将在标准化操作程序下进行，通过支持临床试验来磨练 符合HIPAA。BTM数据库将用于存储加州大学洛杉矶分校和加州大学洛杉矶分校的样品库存 加州大学旧金山分校和相关数据，并向所有私人投资主任和工作人员开放。伊兰核心实验室的理念 将使用管理软件促进工作流程和向数据管理部门报告电子数据 和分析核心B.具体目标3.向绩效指标和工作人员提供样本管理方面的教育， 免疫评估技术和数据分析/解释，并传播新的 在重债穷国联盟中的知识。核心将教育调查人员关于技术、他们的 这将有助于减少项目的应用和限制，并将促进项目和核心之间的沟通与合作。