Core B: Immune Assessment

核心 B：免疫评估

• 批准号: 10225359
• 负责人: Maura Rossetti
• 金额: $ 7.32万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10225359
• 关键词: Address; Area; Biological Assay; Biopsy; Blood; Cell physiology; Clinical; Clinical Trials; Collaborations; Color; Communication; Computer software; Coupled; Cytomegalovirus; Cytomegalovirus Infections; Data; Data Analyses; Data Reporting; Databases; Education; Ensure; Equipment and supply inventories; Flow Cytometry; GEM gene; Goals; Health Insurance Portability and Accountability Act; Human Resources; Immune; Immune response; Immunoassay; Immunophenotyping; Informatics; Kidney Transplantation; Knowledge; Laboratories; Leadership; Liquid substance; Measures; Outcome; Performance; Periodicity; Peripheral Blood Mononuclear Cell; Plasma; Procedures; Protocols documentation; Reagent; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Serum; Specimen; Standardization; Systems Biology; Technical Expertise; Technology; Technology Assessment; Testing; Tissues; Training; Transplant Recipients; Urine; Viremia; Work; adaptive immune response; base; biobank; clinical phenotype; cost; data exchange; data management; data sharing; design; electronic data; experimental study; heart allograft; immunological diversity; innovation; kidney allograft; lung allograft; meetings; new technology; programs; reagent standard; repository; synergism

CORE B: Immune Assessment SUMMARY/ABSTRACT The Immune Assessment Core B is essential to the research goals of the Project PIs collaborating in this HIPC Consortium. The proposed research aims to characterize the innate and adaptive immune response to CMV in recipients of heart, lung and renal allografts. Core B will provide skilled technical and scientific expertise to enable the PIs to achieve their research goals through the following specific Aims. Specific Aim 1. To provide professional expertise in the design and implementation of assays to measure immune cell function. The Core has validated standardized 14-color flow cytometry, multiplex immunoassays, and KIR typing assays to support the research of Projects 1 and 2. The Core will perform the assays and interpret the data. The IAC will ensure consistent and reliable intra- and inter-assay performance using standardized protocols, reagents and data interpretation. The core will coordinate experiments among the projects with the goal of maximizing testing uniformity and cost reduction. Specific Aim 2. To provide standardized sample processing, maintain a centralized biorepository of tissue, serum, plasma, peripheral blood mononuclear cells and urine, and enable efficient sample and data sharing. Processing and storage of graft biopsies, PBMCs, serum/plasma will be performed under standardized operating procedures honed by supporting clinical trials to be HIPAA compliant. The BTM database will be used to house the sample inventories at both UCLA and UCSF and associated data and made accessible to all PIs and staff. The Idea Elan core laboratory management software will be used to facilitate work flow and electronic data reporting to Data Management and Analysis Core B. Specific Aim 3. To provide education of PIs and staff in sample management, immune assessment technologies and data analysis/interpretation, and to disseminate the new knowledge in the HIPC Consortium. The Core will educate the investigators about technologies, their applications and limitations, and will promote communication and collaboration among projects and cores.

核心 B：免疫评估 摘要/摘要 免疫评估核心 B 对于本重债穷国合作项目的研究目标至关重要 财团。拟议的研究旨在表征 CMV 的先天性和适应性免疫反应 心脏、肺和肾同种异体移植物的接受者。核心 B 将提供熟练的技术和科学专业知识 使 PI 能够通过以下具体目标实现其研究目标。具体目标 1. 提供 设计和实施免疫细胞功能检测的专业知识。 Core 已验证标准化 14 色流式细胞术、多重免疫分析和 KIR 分型分析 支持项目 1 和 2 的研究。核心将进行分析并解释数据。国际咨询委员会 将使用标准化方案、试剂确保一致且可靠的测定内和测定间性能 和数据解释。核心将协调项目之间的实验，目标​​是最大化 测试一致性和降低成本。具体目标2.提供标准化样品处理， 维持组织、血清、血浆、外周血单核细胞的集中生物储存库 尿液，并实现高效的样本和数据共享。移植活检、PBMC 的处理和储存， 血清/血浆将按照通过支持临床试验而完善的标准化操作程序进行 符合 HIPAA 要求。 BTM 数据库将用于存放加州大学洛杉矶分校和加州大学洛杉矶分校的样本库存。 UCSF 和相关数据可供所有 PI 和员工访问。 Idea Elan核心实验室 管理软件将用于促进工作流程和向数据管理部门报告电子数据 和分析核心 B. 具体目标 3. 为 PI 和工作人员提供样本管理方面的教育， 免疫评估技术和数据分析/解释，并传播新的 重债穷国联盟的知识。核心将教育调查人员有关技术、他们的 应用和限制，并将促进项目和核心之间的沟通和协作。