Generation of a Complete Set of Precise Null Bar-Coded Deletion Mutants of Mycobacterium tuberculosis

结核分枝杆菌一整套精确空条形码缺失突变体的生成

• 批准号: 9754771
• 负责人: WILLIAM Robert JACOBS
• 金额: $ 57.21万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9754771
• 关键词: Alleles; Attenuated; Bacillus (bacterium); Bacteriophages; Bar Codes; Biological Markers; Biology; Cessation of life; Cloning; Collection; Communities; Complement; Containment; DNA; DNA Sequence; Data; Databases; Drug Targeting; Electroporation; Engineering; Epidemic; Escherichia coli; Evaluation; Extreme drug resistant tuberculosis; Foundations; Freeze Drying; Frequencies; Gene Deletion; Gene Targeting; Generations; Genes; Genome; Genotype; Glycerol; Goals; Gold; Growth; HIV; In Vitro; International; Interruption; Laboratories; Libraries; Metabolism; Methodology; Modeling; Molecular Genetics; Morbidity - disease rate; Multidrug-Resistant Tuberculosis; Mus; Mycobacteriophages; Mycobacterium smegmatis; Mycobacterium tuberculosis; Nucleotides; Online Systems; Phenotype; Physiology; Plasmid Cloning Vector; Plasmids; Production; Proteins; Protocols documentation; Quality Control; Reagent; Recombinants; Research; Resources; Technology; Tuberculosis; Vaccines; Virulent; base; chemotherapy; cost effective; fitness; genome-wide; global health; knockout gene; mortality; mutant; nonhuman primate; pathogen; reverse genetics; tuberculosis diagnostics; vector

Abstract Tuberculosis (TB) continues to be a major global health problem with 9 million new cases per year resulting in 1.7 million deaths [1], due in large part to the HIV epidemic and emergence of multi- and extensively drug resistant TB strains (MDR- and XDR-TB) that have confounded TB control. There will be no lasting reduction of TB morbidity and mortality without rapid and cost-effective TB diagnostics, efficacious vaccines, and shortened, well-tolerated chemotherapy, which can only be developed on the foundation of understanding the biology of the tubercle bacilli and subsequent host-pathogen interactions. The goal of this proposal is to accelerate TB research through the completion and distribution of these valuable precise null deletion M. tuberculosis gene knockout resources to the international TB research community. We will partner with BEI for distribution of reagents.

摘要 结核病（TB）仍然是一个主要的全球健康问题，有900万新病例 每年造成170万人死亡[1]，主要原因是艾滋病毒流行， 出现多重和广泛耐药结核菌株（MDR和XDR-TB）， 使结核病控制工作陷入困境。结核病发病率不会持久下降， 没有快速和具有成本效益的结核病诊断、有效的疫苗， 缩短的，耐受性良好的化疗，只能在基础上开发 了解结核杆菌的生物学和随后的宿主病原体 交互.该提案的目标是通过完成 以及这些有价值的精确空缺失M的分布。结核病基因敲除 为国际结核病研究界提供资源。我们将与BEI合作， 分发试剂。