Generation of a Complete Set of Precise Null Bar-Coded Deletion Mutants of Mycobacterium tuberculosis

摘要 结核病(TB)仍然是一个主要的全球健康问题，新增病例达900万例 每年造成170万人死亡[1]，很大程度上是由于艾滋病毒流行和 出现耐多药和广泛耐药的结核病株(耐多药和广泛耐药结核) 已经扰乱了结核病控制。结核病发病率不会持续下降，而且 如果没有快速和具有成本效益的结核病诊断、有效的疫苗和 缩短、耐受性好的化疗，只能在此基础上开发 了解结核杆菌和随后的宿主病原体的生物学 互动。这项提案的目标是通过完成 以及这些有价值的精确缺失结核分枝杆菌基因敲除的分布 向国际结核病研究界提供资源。我们将与BEI合作 试剂的分布。

Abstract Tuberculosis (TB) continues to be a major global health problem with 9 million new cases per year resulting in 1.7 million deaths [1], due in large part to the HIV epidemic and emergence of multi- and extensively drug resistant TB strains (MDR- and XDR-TB) that have confounded TB control. There will be no lasting reduction of TB morbidity and mortality without rapid and cost-effective TB diagnostics, efficacious vaccines, and shortened, well-tolerated chemotherapy, which can only be developed on the foundation of understanding the biology of the tubercle bacilli and subsequent host-pathogen interactions. The goal of this proposal is to accelerate TB research through the completion and distribution of these valuable precise null deletion M. tuberculosis gene knockout resources to the international TB research community. We will partner with BEI for distribution of reagents.