Quantitative Imaging of Mouse Brain Development

小鼠大脑发育的定量成像

基本信息

项目摘要

Abstract: Brain development is a highly dynamic yet precisely orchestrated process. Using genetically modified mouse models, we are in the process of unveiling the complex mechanisms that control critical cellular events in the developing brain. High-throughput imaging tools will greatly benefit studies in this area by charactering brain phenotypes at the macroscopic/mesoscopic levels and directing subsequent examinations at the cellular and molecular levels. In this project, multiple novel magnetic resonance imaging (MRI) techniques will be developed to non-invasively exam a wide range of phenotypes in the developing mouse brain from mid- embryonic stage to adolescence. The target phenotypes include macroscopic brain morphology and structural connectivity, microstructural organization, neuronal migration and differentiation, and postnatal brain activity. The proposed techniques include fast imaging sequences, novel image contrasts, optimized imaging coils/holder, and image analysis tools, many of which stem from on our existing expertise. In Aim 1, we will develop imaging tools to achieve high-throughput in vivo multi-contrast MRI of the developing mouse brain. We will collect multi-contrast MRI data to construct an in vivo MRI atlas of the developing mouse brain to assist mouse brain phenotype analysis and use the sas4-/- mouse, a model of microcephaly, to test the performance of the technique. In Aim 2, we will use novel diffusion MRI techniques to characterize macroscopic morphology, connectivity, and microstructural organization in the developing brain. In particular, high angular resolution diffusion imaging (HARDI) will be used to resolve complex tissue microstructural organization and reconstruct connectivity between major brain regions, and the new oscillating gradient diffusion MRI technique will be used to exam changes in cellularity in the developing cortex associated with neuronal migration. Detailed examination of the relationships between diffusion MRI-based markers and specific histological markers will determine their sensitivity to the underlying developmental processes. In Aim 3, we will use novel Manganese (Mn2+)-enhanced MRI as another tissue contrast, which reflects postnatal brain activity and potentially neuronal differentiation in the embryonic brain, to examine the developing mouse brain. We will examine the contrast patterns of Mn2+-enhanced MRI in the embryonic and neonatal mouse brain with the patterns of neuronal differentiation observed in histological data to determine the sensitivity of Mn2+-enhanced MRI to neuronal differentiation. In addition, we will investigate potential toxic effects of Mn2+ on brain development, and establish protocols that minimize these effects. In Aims 2 and 3, the techniques will also be used to characterize three mutant mouse models with abnormal brain phenotypes resulting from defects in neuronal migration and differentiation. The imaging techniques and knowledge gained in this project will greatly enhance our ability to quantitatively characterize the phenotypes of mutant mouse models in order to achieve a deep understanding of brain development and disorders.
文摘:

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Daniel H Turnbull其他文献

Brain folding is initiated by mechanical constraints without a cellular pre-pattern
大脑折叠是由机械约束引发的,没有细胞预模式
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    7.7
  • 作者:
    Andrew K Lawton;Tyler Engstrom;Daniel Rohrbach;Masaaki Omura;Daniel H Turnbull;Jonathan Mamou;Teng Zhang;J. M. Schwarz;Alexandra L Joyner,
  • 通讯作者:
    Alexandra L Joyner,
Doppler Characterization of Murine Embryonic Umbilical Blood Flow: Insights into Developing Embryonic-Placental Circulation and Cardiac Function
  • DOI:
    10.1203/00006450-199904020-00175
  • 发表时间:
    1999-04-01
  • 期刊:
  • 影响因子:
    3.100
  • 作者:
    Colin K Phoon;Orlando Aristizabal;Daniel H Turnbull
  • 通讯作者:
    Daniel H Turnbull

Daniel H Turnbull的其他文献

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{{ truncateString('Daniel H Turnbull', 18)}}的其他基金

Quantitative Imaging of Mouse Brain Development
小鼠大脑发育的定量成像
  • 批准号:
    10116502
  • 财政年份:
    2018
  • 资助金额:
    $ 61.9万
  • 项目类别:
Ultrasound and MR Imaging of Mouse Brain Development.
小鼠大脑发育的超声和磁共振成像。
  • 批准号:
    8664143
  • 财政年份:
    2013
  • 资助金额:
    $ 61.9万
  • 项目类别:
Molecular UBM and MRI of Vascular Development
血管发育的分子 UBM 和 MRI
  • 批准号:
    8769741
  • 财政年份:
    2013
  • 资助金额:
    $ 61.9万
  • 项目类别:
MRI Tracking of Stem Cell Migration During Brain Injury
脑损伤期间干细胞迁移的 MRI 追踪
  • 批准号:
    7895361
  • 财政年份:
    2010
  • 资助金额:
    $ 61.9万
  • 项目类别:
MRI Tracking of Stem Cell Migration During Brain Injury
脑损伤期间干细胞迁移的 MRI 追踪
  • 批准号:
    8018555
  • 财政年份:
    2010
  • 资助金额:
    $ 61.9万
  • 项目类别:
Mouse Imaging
小鼠成像
  • 批准号:
    7714225
  • 财政年份:
    2008
  • 资助金额:
    $ 61.9万
  • 项目类别:
7-TESLA MR MICRO-IMAGING: BRAIN DVMT
7-TESLA MR 显微成像:大脑 DVMT
  • 批准号:
    7166620
  • 财政年份:
    2005
  • 资助金额:
    $ 61.9万
  • 项目类别:
7-Tesla MR Micro-imaging Console
7-Tesla MR 微型成像控制台
  • 批准号:
    6877608
  • 财政年份:
    2005
  • 资助金额:
    $ 61.9万
  • 项目类别:
7-TESLA MR MICRO-IMAGING: CARDIOVASCULAR DVMT
7-TESLA MR 显微成像:心血管 DVMT
  • 批准号:
    7166616
  • 财政年份:
    2005
  • 资助金额:
    $ 61.9万
  • 项目类别:
7-TESLA MR MICRO-IMAGING: ALZHEIMER'S DISEASE
7-TESLA MR 显微成像:阿尔茨海默病
  • 批准号:
    7166617
  • 财政年份:
    2005
  • 资助金额:
    $ 61.9万
  • 项目类别:

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