Diversity of the Mosquito Microbiota and its Influence on Pathogen Transmission

蚊子微生物群的多样性及其对病原体传播的影响

• 批准号: 9764449
• 负责人: Matthew C I Medeiros
• 金额: $ 20.76万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9764449
• 关键词: Address; Aedes; Arboviruses; Biological Sciences; Blood; Chikungunya virus; Communities; Competence; Culex (Genus); Culicidae; Dengue; Dengue Virus; Development; Digestion; Disease; Fertility; Food; Genes; Genetic; Genotype; Hawaiian population; Health; Human; Immune system; Individual; Infection; Intervention; Knowledge; Laboratories; Larva; Link; Malaria; Medical; Metabolism; Microbe; Modeling; Mosquito-borne infectious disease; Nature; Outcome; Parasite resistance; Parasites; Phenotype; Population; Public Health; Research; Resource Sharing; Role; Shapes; Structure; Surveys; Temperature; Testing; Time; Variant; Work; Zika Virus; base; density; design; differential expression; disease transmission; experimental study; frontier; human disease; human pathogen; life history; microbial; microbiome; microbiota; microbiota profiles; mosquito-borne pathogen; novel; novel strategies; pathogen; professor; response; symbiont; trait; translational impact; transmission process; vector; vector mosquito

Project Summary – PROJECT 2: JI, Matthew Medeiros The symbiotic microbiota of mosquitoes has pervasive effects on host traits, including those of vectors of important human diseases like dengue virus, chikungunya virus, and Zika virus. Coordinated interactions between mosquitoes and their microbiota shape mosquito metabolism, development, fecundity, survival, and immune systems. These effects on individual vectors may scale to influence the transmission of infectious human pathogens at the population and community levels. While the mosquito microbiota is known to modulate the capacity of vectors to sustain human pathogen transmission, the factors that contribute to variation in the composition and density of the mosquito microbiota across individual vectors and vector populations remain poorly resolved. The central hypothesis of this proposal posits that (i) environmental, ecological, and genetic forces drive the assembly of the mosquito microbiota; (ii) differences in these forces over time and space produce variation in the microbiota between individual vectors; and, (iii) this variation between individual vectors scales to impact disease transmission among hosts, including humans. To test this hypothesis, this study will employ numerous carefully designed experiments in both field and controlled laboratory settings. These experiments aim to clarify the role of environmental, ecological, and genetic factors in shaping mosquito microbiota diversity using Hawaiian populations of two medically important vectors, Culex quinquefasciatus and Aedes albopictus. In addition, it will investigate the consequences of this diversity on dengue transmission, the most relevant mosquito-borne pathogen in Hawai`i. Expected outcomes of this work include a better model for the assembly of the mosquito microbiota and its cumulative impact on pathogen transmission. Ultimately, this study is expected to have a positive translational impact by informing the development of novel, microbe-based strategies to mitigate mosquito-borne diseases of public health concern.

项目概要-项目2：JI，Matthew Medeiros 蚊子的共生微生物群对宿主特征具有普遍影响，包括蚊子的媒介特征。 重要的人类疾病，如登革热病毒、基孔肯雅病毒和寨卡病毒。协调互动 蚊子与其微生物群之间的关系塑造了蚊子的新陈代谢、发育、繁殖力、生存和 免疫系统这些对单个媒介的影响可能会扩大到影响传染性疾病的传播。 人类病原体在人群和社区层面。虽然蚊子的微生物群 调节病媒维持人类病原体传播的能力， 蚊子微生物群的组成和密度在个体媒介和媒介之间的变化 人口问题仍然没有得到妥善解决。这一建议的核心假设是：（一）环境， 生态和遗传力量驱动蚊子微生物群的组装;（ii）这些力量的差异 随着时间和空间的推移，在个体载体之间的微生物群中产生变化;以及（iii）这种变化 个体病媒之间的相互作用影响着疾病在包括人类在内的宿主之间的传播。为了验证这一 假设，这项研究将采用许多精心设计的实验，在两个领域和控制 实验室设置。这些实验旨在阐明环境、生态和遗传因素的作用 利用夏威夷种群的两种医学上重要的媒介库蚊来塑造蚊子微生物群多样性 致倦伊蚊和白纹伊蚊。此外，它还将调查这种多样性对 登革热传播，夏威夷最相关的蚊媒病原体。这项工作的预期成果 包括一个更好的蚊子微生物群组装模型及其对病原体的累积影响， 传输最终，这项研究预计将通过告知 制定新的、基于微生物的战略，以减轻蚊子传播的公共卫生疾病。