Imaging Core

项目摘要 成像核心是Sanford Research内部的一个既定实体，并在阶段期间提供整体技术支持。 I支持儿科研究中心维护和支持显微镜成像 提供先进显微技术和成像相关分析方面的专业知识 项目负责人和其他桑福德研究学院的数据。本申请的目的是确保 核心业务的继续，以扩大和增强核心的能力，并确保质量 为儿科研究中心的新研究者提供成像核心服务，以及 继续支持其他桑福德研究中心的研究人员。为实现这一目标， 提出了具体目标： 目标1：扩展成像核心功能。我们的目标是扩大我们的核心服务， Thermo CellInsight CX 7高含量筛选系统，用于快速定量各种细胞 参数，显著减少定量组织学所需的人力/时间。我们将 增加额外的技术支持（与功能基因组学和生物信息学核心结合）， 提供分析师支持。此次扩建将满足儿科中心项目负责人的需求， 疾病，其他桑福德研究所的调查人员，以及来自地区机构的科学家，包括那些在 其他NIH IdEA资助的网站，并将允许核心继续提供培训，支持和维护 其设备。 目标2：在项目设计、文稿编写和资金申请方面提供援助。 在项目设计、方案制定、执行和 故障排除核心负责人和经理将与调查人员协商，提供技术方面的专业知识， 接近。拟议的人员扩充将使我们能够提供详细的实验方案， 核心的手稿和资金申请。 目标3：创建可持续的成像核心。我们的长期目标是建立一个可持续的核心 能够完全支持我们科学家的研究需求。核心将开发一种收费服务 结构，沿着机构支持，将在COBRE支持期之后维持Core。 这些建议的目标如能成功落实，便可扩展和加强服务 这将满足我们不断增长的技术需求。通过额外的技术支持和 通过增强设备，核心将在科学项目的所有阶段与研究人员进行有效互动， 从而实现其目的：支持核心用户的目标，即独立科学家寻求发现 人类健康和疾病的机制。

PROJECT SUMMARY The Imaging Core is an established entity within Sanford Research and provides integral technical during Phase I support of the Center for Pediatric Research in the maintenance and supports of microscopy-oriented imaging equipment and in the delivery of expertise in advanced microscopy techniques and analyses of imaging related data for Project Leaders and other Sanford Research faculty. The objective of the current application is to secure the continuation of Core's operations, to augment and enhance Core's capabilities, and to ensure the quality services of the Imaging Core to the new investigators within the Center for Pediatric Research, as well as continued Core support of the other Sanford Research investigators. To achieve this objective, the following specific aims are proposed: Aim 1: To expand the Imaging Core capabilities. We aim to expand our Core services to include support of a Thermo CellInsight CX7 High Content Screening system for rapid quantification of various cellular parameters, significantly reducing the amount of people-power/time needed for quantitative histology. We will add additional technical support (in conjunction with the Functional Genomics and Bioinformatics Core) that can provided analyst support. This expansion will meet the needs of the project leaders within the Center for Pediatric Disease, the other Sanford Research investigators, and scientists from regional institutions, including those at other NIH IdEA-funded sites, and will allow the Core to continue providing training, support and maintenance of its equipment. Aim 2: To provide assistance with project design, manuscript preparation and funding applications. The Core will directly interface with investigators during project design, protocol establishment, execution, and troubleshooting. The Core leads and manager will consult with investigators, offering an expertise in technical approaches. The proposed personnel expansion will enable us to deliver detailed experimental protocols used by the Core for manuscripts and funding applications. Aim 3: To create a sustainable Imaging Core. Our long-term goal is to establish a sustainable Core capable of fully supporting the research demands of our scientists. The Core will develop a fee-for-service structure, which, along with an institutional support, will sustain Core beyond the period of CoBRE support. The successful implementation of these proposed aims will translate into expanded and enhanced services by the Imaging Core, which will meet our growing technical demands. With the additional technical support and enhanced equipment, the Core will efficiently interact with the investigators during all stages of scientific projects, thus fulfilling its purpose: to support the goals of Core users, namely independent scientists seeking to uncover the mechanisms of human health and disease.