Tools and Methods for Producing High Quality Functional Protein Microarrays for Biomarker Discovery

用于生产用于生物标志物发现的高质量功能蛋白微阵列的工具和方法

• 批准号: 9767874
• 负责人: JOSHUA LABAER
• 金额: $ 34.96万
• 依托单位: SPOC PROTEOMICS INC.
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9767874
• 关键词: Animal Model; Area; Arizona; Autoimmune Diseases; Automation; Biological Assay; Biological Markers; Budgets; Capital; Cells; Clinical; Communicable Diseases; Complex; Computers and Advanced Instrumentation; Consultations; Consumption; Core Facility; Custom; DNA; DNA Microarray Chip; Development; Disease; Drug Interactions; Enzymes; Generations; Genes; Genetic Transcription; Glass; Hour; Human; In Situ; In Vitro; Incidence; Institutes; Laboratories; Legal patent; Letters; Liquid substance; Malignant Neoplasms; Manuals; Mechanics; Methods; Monitor; Nucleic Acids; Pathology; Phase; Phosphorylation; Plasmids; Post-Translational Protein Processing; Preclinical Drug Development; Procedures; Production; Prognostic Marker; Protein Array; Protein Microarray Assay; Protein Microchips; Proteins; Proteome; Proteomics; Proto-Oncogene Proteins c-jun; Reproducibility; Reproducibility of Results; Research; Role; Ships; Signal Pathway; Slide; Small Business Innovation Research Grant; Speed; Spottings; System; TP53 gene; Technology; Testing; Time; Translations; Universities; Validation; Variant; biomarker discovery; cancer biomarkers; clinical biomarkers; commercialization; cross reactivity; density; design; disorder prevention; improved; innovation; instrument; instrumentation; interest; off-patent; outreach; preclinical trial; product development; programs; protein function; protein protein interaction; prototype; screening; small molecule; tool

Abstract: Functional protein microarrays find applications in high throughput disease biomarker discovery, understanding the incidence and role of post translational modifications (PTMs) in disease pathology, investigating activity and function of enzymes, protein–protein and protein– small molecule interactions, among others. Nucleic Acid Programmable Protein Array (NAPPA) technology is a robust method for producing cell-free in situ expressed functional protein microarrays in human lysate, freshly expressed and displayed at the time of assay, and has been widely applied in disease biomarker discovery research over the last decade. We aim to develop an advanced instrument for automating the production of NAPPA IPC (Isolated Protein Capture) high density protein microarrays on glass slides. Once the instrument is developed, we expect similar improvements in simplicity, speed and quality of NAPPA protein microarray assays as was seen when DNA microarrays were transferred from manual to automated workstations. In addition to clinical biomarker research, it is an ideal platform for profiling for disease biomarkers in animal models, a subset of which can then be monitored as prognostic biomarkers, indicators of end points in drug development pre-clinical trials.

摘要：功能蛋白微阵列在高通量疾病生物标志物中得到应用 发现、理解翻译后修饰 (PTM) 的发生率和作用 疾病病理学，研究酶、蛋白质-蛋白质和蛋白质-的活性和功能 小分子相互作用等。核酸可编程蛋白质阵列 (NAPPA) 技术是生产无细胞原位表达功能蛋白的可靠方法 人类裂解物中的微阵列，在检测时新鲜表达和展示，并且具有 在过去十年中被广泛应用于疾病生物标志物发现研究。我们的目标是 开发一种先进的仪器，用于自动化生产 NAPPA IPC（分离蛋白） 在载玻片上捕获）高密度蛋白质微阵列。一旦仪器开发出来，我们 期望 NAPPA 蛋白质微阵列在简单性、速度和质量方面有类似的改进 当 DNA 微阵列从手动转移到自动化时可以看到 工作站。除了临床生物标志物研究之外，它还是一个理想的分析平台 动物模型中的疾病生物标志物，其中的一部分可以作为预后进行监测 生物标志物，药物开发临床前试验终点指标。