NIGMS National and Regional Resources - DNASU

项目总结 DNASU是一个有11年历史的非营利性、基于学术的存储库，它提供学术界、工业界和 政府拥有各种各样的质粒，包括专门的载体、调控的基因序列和 来自1,269个生物体的基因，有许多完整的基因组集合，包括第一套完整的人类基因组。 用户通过我们的网站https://dnasu.org/，访问存储库，他们可以：查看相关克隆、 基因和载体信息；订购克隆；以及利用链接来访问外部资源，如协议 和出版物。DNASU通过我们的高通量克隆，通过内部生产获得了质粒 管道和外部研究小组的沉积。此外，我们与蛋白质配对 结构倡议(PSI)，我们对其进行了全长序列验证和对质粒的注释 在分发它们之前，从多个PSI中心。当用户访问DNASU请求时启动分发 选定的构件(S)。技术人员继代培养和运输甘油库存，象征性地收取补充费。DNASU 维护和存档超过260,000个独特的质粒。DNASU网站平均有3,000个独特的 每月有访客，收到5,000份订单，每年向世界各地的科学家分发超过35,000份质粒。 DNASU通过PSI计划从NIGMS获得资金，该计划使&gt；93,000得以增加 为其收集的质粒和开发用于定位和使用克隆的软件工具，从而创造了一种 全球独一无二的资源。我们还从NIGMS Legacy计划获得资金，以改进 基础设施、成员集合、存储库的可见性和可持续性。我们已经取得了重大进展 取得进展，以创建一项自我维持的努力，使我们可以继续从现有的 收集。然而，我们分发的克隆数量虽然很大，但与 我们必须保持收藏，所以我们的销售不支持持续的数据库/计算机编程， 维护和更新我们的收藏，或购买新的收藏。我们认识到，科学 社区需要一个像我们这样全面的档案网站，以便继续扩大科学知识 关于许多尚未被研究的蛋白质。我们正在寻求资金来支持我们的运营和 客户支持：1)通过精炼湿法实验室维护和改进物理质粒库 方法，将目标集合更新为当前表达载体，刷新克隆以确保生存能力，以及 协助收购孤儿藏品；2)增强DNASU的生物信息学能力 目前采用现代硬件、软件和操作系统，并加强DNASU数据库和 用于物理克隆和相关生物信息的序列注释的生物信息学管道； 以及3)通过重新构建DNASU的订单接口来改善客户体验和外联 安置，改善技术支持，并通过社交媒体支持吸引科学家。

PROJECT SUMMARY DNASU is a 11 year old non-profit, academic-based repository that provides academia, industry and the government with a wide variety of plasmids including specialized vectors, regulatory genetic sequences, and genes from 1,269 organisms, with many complete genome collections, including the first complete human set. Users access the repository through our website, https://dnasu.org/, where they can: view pertinent clone, gene and vector information; order clones; and utilize link-outs to reach external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our high throughput cloning pipeline and through deposition by outside research groups. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full-length sequence verification and annotation on plasmids from multiple PSI centers before distributing them. Distribution is initiated when a user visits DNASU to request selected construct(s). Technicians subculture and ship the glycerol stocks for a nominal re-charge fee. DNASU maintains and archives over 260,000 unique plasmids. The DNASU website sees an average of 3,000 unique visitors per month, receives 5,000 orders and distributes over 35,000 plasmids per year to scientists worldwide. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >93,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. We also received funds from NIGMS Legacy program to improve the infrastructure, member collection, visibility and sustainability of the repository. We have made significant inroads to create a self-sustaining effort whereby we can continue to distribute clones from our existing collection. However, the number of clones we distribute, although large, is small relative to the massive size of the collection we must maintain, so our sales do not support continued database/computer programming, upkeep and updating of our collection, or acquisition of new collections. We recognize that the scientific community needs a comprehensive archival site like ours in order to continue expanding scientific knowledge on the many proteins that have not yet been studied. We are seeking funding to support our operations and customer support to: 1) Maintain and Improve the Physical Plasmid Repository by refining wet lab methods, updating targeted collections to current expression vectors, refreshing clones to ensure viability, and assisting with acquisition of orphaned collections; 2) Enhance Bioinformatics Capabilities of DNASU to stay current with modern hardware, software and operating systems, and to enhance the DNASU database and bioinformatics pipelines for sequence annotation of the physical clones and the related biological information; and 3) Improve Customer Experience and Outreach by re-constructing the DNASU interface for order placement, improving technical support, and engaging scientists through social media support.