NIGMS National and Regional Resources - DNASU

项目摘要 DNASU是一个有11年历史的非盈利性学术知识库，为学术界、工业界和 政府拥有各种各样的质粒，包括专门的载体，调控基因序列， 来自1，269种生物的基因，拥有许多完整的基因组集合，包括第一个完整的人类集合。 用户通过我们的网站https：//dnasu.org/访问存储库，在那里他们可以：查看相关克隆， 基因和载体信息;订购克隆;并利用链接输出来访问外部资源，如协议 和出版物。DNASU通过我们的高通量克隆通过内部生产获得质粒 管道和通过沉积由外部研究小组。此外，我们还与Protein 结构倡议组织（PSI），我们为他们进行了质粒全长序列验证和注释 从多个防扩散安全中心收集信息当用户访问DNASU请求时， 选定的结构。技术人员对甘油储备液进行继代培养和运输，象征性地收取再收费。DNASU 保存并存档了超过26万个独特的质粒DNASU网站上平均有3,000个独特的 每月接待5，000名访客，每年向世界各地的科学家分发35，000多份质粒。 DNASU通过PSI倡议获得了NIGMS的资助，该倡议使其增加了> 93，000人 质粒到其收集和软件工具的开发定位和使用克隆，从而创建一个 全球独一无二的资源。我们还收到了NIGMS遗产计划的资金，以改善 存储库的基础设施、成员收藏、可见性和可持续性。我们取得了重大 取得进展，创造一个自我维持的努力，使我们能够继续分发克隆从我们现有的 收藏.然而，我们分发的克隆数量虽然很大，但相对于庞大的 我们必须维护的集合，因此我们的销售不支持持续的数据库/计算机编程， 维护和更新我们的收藏，或购买新的收藏。我们认识到， 社区需要一个像我们这样的综合档案网站，以继续扩大科学知识 许多尚未被研究的蛋白质。我们正在寻求资金来支持我们的业务， 客户支持：1）通过改进湿实验室来维护和改进物理质粒库 方法，将目标集合更新为当前表达载体，刷新克隆以确保活力，以及 协助收购孤儿收藏品; 2）加强DNASU的生物信息学能力，以保持 目前与现代化的硬件，软件和操作系统，并加强DNASU数据库和 用于物理克隆的序列注释和相关生物信息的生物信息学管道; 以及3）通过重新构建DNASU订单界面来改善客户体验和推广 安置，改善技术支持，并通过社会媒体支持吸引科学家。

PROJECT SUMMARY DNASU is a 11 year old non-profit, academic-based repository that provides academia, industry and the government with a wide variety of plasmids including specialized vectors, regulatory genetic sequences, and genes from 1,269 organisms, with many complete genome collections, including the first complete human set. Users access the repository through our website, https://dnasu.org/, where they can: view pertinent clone, gene and vector information; order clones; and utilize link-outs to reach external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our high throughput cloning pipeline and through deposition by outside research groups. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full-length sequence verification and annotation on plasmids from multiple PSI centers before distributing them. Distribution is initiated when a user visits DNASU to request selected construct(s). Technicians subculture and ship the glycerol stocks for a nominal re-charge fee. DNASU maintains and archives over 260,000 unique plasmids. The DNASU website sees an average of 3,000 unique visitors per month, receives 5,000 orders and distributes over 35,000 plasmids per year to scientists worldwide. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >93,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. We also received funds from NIGMS Legacy program to improve the infrastructure, member collection, visibility and sustainability of the repository. We have made significant inroads to create a self-sustaining effort whereby we can continue to distribute clones from our existing collection. However, the number of clones we distribute, although large, is small relative to the massive size of the collection we must maintain, so our sales do not support continued database/computer programming, upkeep and updating of our collection, or acquisition of new collections. We recognize that the scientific community needs a comprehensive archival site like ours in order to continue expanding scientific knowledge on the many proteins that have not yet been studied. We are seeking funding to support our operations and customer support to: 1) Maintain and Improve the Physical Plasmid Repository by refining wet lab methods, updating targeted collections to current expression vectors, refreshing clones to ensure viability, and assisting with acquisition of orphaned collections; 2) Enhance Bioinformatics Capabilities of DNASU to stay current with modern hardware, software and operating systems, and to enhance the DNASU database and bioinformatics pipelines for sequence annotation of the physical clones and the related biological information; and 3) Improve Customer Experience and Outreach by re-constructing the DNASU interface for order placement, improving technical support, and engaging scientists through social media support.