DNASU, the Plasmid Materials Repository, 2020 and Beyond

项目摘要 DNASU是非营利性的信息库，为学术界、政府和工业界提供基于DNA的 资源，其中包括各种不同的质粒，包括专门的实验载体，调节 基因序列，以及来自1100多个生物的基因。储存在DNASU的质粒需要大量的 生产时间和成本，DNASU寻求避免重复工作，并通过共享来加速研究 公开地告诉他们。此外，DNASU安全地存档了质粒并收集了每个质粒的详细注释 统一且易于访问的格式。用户通过我们的网站https://dnasu.org/，访问存储库 在那里他们可以查看相关的克隆、基因和载体信息，以及到外部资源的链接 例如协议和出版物。DNASU通过我们自己的内部生产获得了质粒 高通量克隆流水线和外部研究小组的沉积，包括ORFeome 合作，这是一种跨国合作，寻求建立一套完整的人类基因和 分享它们。此外，我们与蛋白质结构倡议(PSI)合作，我们为该倡议执行了全面的- 对来自PSI中心的质粒进行长度测序和注释，然后将其存放。所有的质粒都是 作为工作集合存储在自动存储检索系统(ASRS)中，并存储在单独位于- 80台⁰C冰柜，用于存档存储。当用户访问DNASU以查找和请求时，将启动分发 精选建设(S)通过互联网。电子请求被传递给ASR，ASR检索克隆 对于以象征性的充值费继代和运输甘油库存的技术员来说。DNASU维护 超过21.9万个独特的质粒，而且还在不断增加。DNASU网站的平均点击量为9000次， 每月收到155份订单，分发超过650个质粒体。我们已经将&gt；300,000个样品发运到 科学家分布在50个国家，其中约80%销往北美。 DNASU通过PSI计划从NIGMS获得资金，使&gt；92,000人得以增加 为其收集的质粒和开发用于定位和使用克隆的软件工具，从而创造了一种 全球独一无二的资源。在接下来的步骤中，我们将努力实现自我可持续性，利用以下几点 针对DNASU运营和维护方面的四个具体目标：1)更新和现代化 DNASU软件符合当前规范；2)完成Human或Feome收集，建立第一个 完整的人类基因组收集；3)加强我们的质粒库维护、归档和规范操作 确保最高质量控制的程序；以及4)建立营销计划以维持社区 连接和提高对DNASU的认识，将其作为科学家的常规工具。我们将结合使用 调整我们的重新收费和增加分销，以实现我们的财务目标并建立 咨询委员会就我们的进展提供有用的反馈。我们从这个票面上寻求资金来帮助实现这一点。 计划建立自给自足，以便DNASU可以在未来许多年继续作为一种资源。

Project Summary DNASU is non-profit repository that provides academia, the government and industry with DNA-based resources, which comprise a wide variety of plasmids including specialized experimental vectors, regulatory genetic sequences, and genes from over 1,100 organisms. The plasmids stored at DNASU required significant time and cost to produce, and DNASU seeks to avoid duplicative effort and to accelerate research by sharing them openly. In addition, DNASU safely archives the plasmids and gathers detailed annotation for each in a uniform and easily accessible format. Users access the repository through our website, https://dnasu.org/, where they can view pertinent clone, gene and vector information, as well as link-outs to external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our own high throughput cloning pipeline and through deposition by outside research groups, including the ORFeome Collaboration, which is a multinational collaboration that seeks to build a complete set of human genes and share them. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full- length sequencing and annotation on plasmids from PSI centers before depositing them. All plasmids are stored in both an automated storage retrieval system (ASRS) as a working collection and in separately located - 80⁰ C freezers for archived storage. Distribution is initiated when a user visits DNASU to find and request selected construct(s) via the Internet. The electronic request is passed to the ASRS, which retrieves the clones for a technician who subcultures and ships the glycerol stocks for a nominal re-charge fee. DNASU maintains over 219,000 unique plasmids, and is constantly growing. The DNASU website sees an average of 9,000 hits, receives 155 orders and distributes over 650 plasmids per month. We have shipped >300,000 samples to scientists in 50 countries, with ~80% of these to North America. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >92,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. For the next steps, we will strive to achieve self-sustainability utilizing the following four specific aims that address the operation and maintenance facets of DNASU: 1) Update and modernize DNASU software to current specifications; 2) Complete the Human ORFeome collection to establish the first complete human gene set collection; 3) Enhance our plasmid maintenance, archives and standard operating procedures to ensure the highest quality control; and 4) Establish a marketing program to maintain community connectivity and increase awareness of DNASU as a routine tool for scientists. We will use a combination of adjustments to our re-charge fees and increased distribution to meet our financial goals and establish an advisory board to provide useful feedback on our progress. We seek funding from this PAR to assist in this plan to establish self-sufficiency so that DNASU can continue as a resource for many years to come.