ERK Kinase Regulation of Insulin Signaling and Inflammation: Honing the Antidiabetic Effects of PPAR-gamma Activation

ERK 激酶对胰岛素信号传导和炎症的调节：增强 PPAR-gamma 激活的抗糖尿病作用

• 批准号: 9894793
• 负责人: ALEXANDER BANKS
• 金额: $ 39.38万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-02-01 至 2021-07-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9894793
• 关键词: Address; Adipocytes; Adipose tissue; Adoptive Transfer; Adverse effects; Affinity; Agonist; Alanine; Alleles; Anti-Inflammatory Agents; Antidiabetic Drugs; Antiinflammatory Effect; Automobile Driving; Biochemical; Bone Marrow; Cardiovascular Diseases; Cells; ChIP-seq; Chronic; DNA Sequence; Data; Death Rate; Development; Diabetes Mellitus; Expression Profiling; Fatty acid glycerol esters; Future; Gene Expression; Gene Expression Profile; Genes; Genetic Transcription; Goals; High Fat Diet; Homeostasis; Hormones; Immune system; Impairment; Inflammation; Inflammatory; Insulin; Insulin Resistance; Intervention; Investigation; Lead; Ligands; Liquid substance; MAP3K1 gene; MEKs; Malignant Neoplasms; Maps; Mediating; Messenger RNA; Metabolic; Modification; Molecular Target; Morbidity - disease rate; Mus; Mutation; Non-Insulin-Dependent Diabetes Mellitus; Obese Mice; Obesity; Outcome; PPAR alpha; PPAR gamma; Pathogenesis; Pathway interactions; Patients; Peripheral; Peroxisome Proliferator-Activated Receptors; Pharmaceutical Preparations; Pharmacology; Phosphorylation; Phosphorylation Inhibition; Phosphotransferases; Physiological; Population; Predisposition; Proteins; Regulation; Regulatory Element; Role; Safety; Serine; Specificity; Stroke; Testing; Therapeutic; Thiazolidinediones; Transcript; Transcriptional Regulation; Weight Gain; Work; adiponectin; blood glucose regulation; bone loss; cytokine; design; energy balance; experimental study; feeding; genetic manipulation; genome-wide; glucose tolerance; improved; inhibitor/antagonist; insulin regulation; insulin sensitivity; insulin sensitizing drugs; insulin signaling; kinase inhibitor; lipid biosynthesis; macrophage; metabolic phenotype; mortality; novel; novel therapeutics; prevent; public health relevance; recruit; side effect

 DESCRIPTION (provided by applicant): The thiazolidinediones (TZDs) are powerful anti-diabetic drugs whose use in treating type 2 diabetes is limited by adverse side effects. The goal of this proposal is a biochemical investigation into a mechanism that separates the positive metabolic effects of TZDs from their side effects, making it possible to design a better class of agents. PPARγ, the molecular target of the TZDs, is a key regulator of systemic insulin sensitivity, adipogenesis, inflammation, and energy homeostasis. In adipose tissue, phosphorylation of PPARγ at serine 273 (S273) is observed shortly after the initiation of high fat diet feeding and increases with progressive obesity. This phosphorylation correlates with dysregulation of PPARγ target genes, such as decreased expression of the insulin-sensitizing hormone adiponectin. ERK is the primary kinase responsible for phosphorylating PPARγ S273, inhibitors of the MEK/ERK kinase pathway block PPARγ S273 phosphorylation. Surprisingly, MEK/ERK inhibitors had potent anti-diabetic effects in obese mice demonstrating markedly improved glucose homeostasis. Similarly, high-affinity ligands of PPARγwhich lack the capacity to promote adipogenesis but still block S273 phosphorylation retain anti-diabetic effects. These two pharmacological interventions blocking PPARγ S273 phosphorylation both promote improved peripheral metabolic homeostasis like the TZDs while also appearing safer as they do not trigger the side effects associated with TZDs. Our hypothesis is that ERK-mediated phosphorylation of PPARγ in obesity and inflammation causes altered impaired glucose homeostasis by targeting adipose tissue transcriptional regulation. We will test this hypothesis using a novel genetically modified mouse where PPARγ cannot be phosphorylated on S273 (S273A). Our preliminary data suggest that blocking this phosphorylation is sufficient to improve insulin sensitivity in obesity. In this proposal we will utilize three approaches to understand the contribution of PPARγ phosphorylation to the pathogenesis of obesity. In Aim 1, we will interrogate the effects of PPARγ S273 phosphorylation on glucose homeostasis and the ability of PPARγ S273A mice to respond to PPARγ ligands. In Aim 2, we will investigate the relative contribution of blocking S273 phosphorylation in the immune system to adipose tissue inflammation and insulin resistance. In Aim 3, we will examine the genome-wide set of mRNA transcripts regulated and cis-regulatory elements bound to either wild-type PPARγ or phosphorylation independent S273A PPARγ. Understanding how obesity and inflammation modulates PPARγ by ERK- mediated phosphorylation will be important for the future design of new therapeutic molecules.