3D Digital Modeling of the Drosphila Brain

果蝇大脑的 3D 数字建模

• 批准号: 7351766
• 负责人: VOLKER HARTENSTEIN
• 金额: $ 26.26万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7351766
• 关键词: Address; Adult; Applications Grants; Archives; Atlases; Axon; Brain; Cell Nucleus; Chromosome Mapping; Communities; Computer Systems; Computer software; Data; Data Set; Deposition; Development; Drosophila genus; Embryo; Future; Gene Expression; Generations; Genes; Genetic; Goals; Grant; Image; Internet; Label; Larva; Lesion; Link; Location; Maps; Mercury; Mind; Modeling; Morphogenesis; Neurobiology; Neuroglia; Neurons; Neurosciences; Numbers; Pattern; Personal Satisfaction; Phenotype; Play; Population; Probability; Process; Pupa; Purpose; Qualifying; Recording of previous events; Research Personnel; Role; Running; Series; Specific qualifier value; Specimen; Stereotyping; System; Time; analytical tool; developmental genetics; digital; digital models; fly; gene function; gene interaction; genetic analysis; member; mutant; neural model; neuroblast; neuronal cell body; relating to nervous system; repository; research study; scaffold; three-dimensional modeling; tool

Digital 3D models play an increasingly important role in neuroscience. Representing three-dimensional scaffolds in which functional data and gene expression data are entered and displayed graphically, the digital models become analytical tools that allow one to address neural connectivity and function, as well as gene function and gene interactions. This grant application proposes to generate a series of standardized digital atlas models of the developing Drosophila brain, a system used by many to investigate the genetic mechanism controlling the formation and function of neuronal circuits. The fly brain is formed by an invariant set of neuroblast lineages which represent structural units in terms of cell body location, axonal projection, and (to an extent that will be addressed in this proposal) connectivity. Axonal and dendritic arborizations, establish morphologically distinct neuropile compartments that are visible from the late embryo towards the adult. Compartments and lineages form a stereotyped pattern that will be captured in the proposed digital models. Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. These models represent an integrated series because the "genetic address" of each neuroblast, defined by the known sets of genes expressed in the early embryonic neuroblast map, will be linked to the population of neurons and their axons modeled for the late embryo, larva and pupa. The goal of this modeling project is to provide a tool shared with the community, allowing to exploit the Drosphila brain more efficiently for developmental-genetic and functional questions. Models of neural lineages will make it possible to phrase specific experiments and to interpret mutant phenotypes.

数字3D模型在神经科学中起着越来越重要的作用。代表三维 脚手架输入并以图形方式输入并显示了功能数据和基因表达数据 模型成为分析工具，可以解决神经连通性和功能以及基因 功能和基因相互作用。该赠款申请建议生成一系列标准化的数字 发育中的果蝇大脑的图集模型，该系统用于研究遗传 控制神经元电路的形成和功能的机制。苍蝇大脑是由不变的 一组神经细胞谱系，代表细胞体位置的结构单位，轴突投影， （在此提案中将解决的范围）连通性。轴突和树枝状动物， 建立形态上不同的神经隔室，从晚期胚胎可见 成人。隔室和血统形成了刻板印象的模式，将在拟议的数字中捕获 型号。考虑到它们对我们和他人的有用性，以及可行性，以下模型是 提议：（1）早期的胚胎神经细胞图，（2）与进化有关的晚期胚胎主要谱系 神经植物，（3）与神经隔室有关的幼虫晚期次生谱系，（4） pupa的道系统和神经隔室。这些模型代表了一个集成的系列 因为每个神经细胞的“遗传地址”，由已知的基因集所定义 早期的胚胎神经细胞图，将与为神经元的群体及其轴突链接 已故的胚胎，幼虫和pupa。该建模项目的目的是提供与该工具共享的工具 社区，可以更有效地利用Ranlyphila大脑来发育遗传和功能 问题。神经谱系的模型将使特定的实验可以说明并解释 突变表型。