权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Validation of Cross-Species Biomarkers of DNA Damage

DNA 损伤跨物种生物标志物的验证

基本信息

批准号：
9769037
负责人：
Jeffrey C Bemis
金额：
$ 61.75万
依托单位：
LITRON LABORATORIES, LTD.
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-01-15 至 2021-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9769037
关键词：
Affect Age Aging Aliquot Antineoplastic Agents Benchmarking Biological Assay Biological Markers Blood Blood Volume Blood specimen Bloom Syndrome Body mass index C-reactive protein Cancer Patient Cells Chromosomal Breaks Code Congenital Abnormality DNA Damage DNA Repair DNA Repair Disorder Data Development Disease Dose Environmental Exposure Erythropoiesis Evaluation Exhibits Family suidae Fanconi&apos s Anemia Flow Cytometry Folic Acid Frequencies Gene Mutation Genes Genomic Instability Genotype Hematopoietic Heritability Human Human Pathology Immunomagnetic Separation Individual Individual Differences Inflammation Inflammatory Bowel Diseases Integration Host Factors International Investigation Kinetics Laboratories Laboratory Animal Models Laboratory Animals Laboratory Study Malignant Neoplasms Measurement Messenger RNA Monitor Mus Mutagens Mutation Nature Newborn Infant Nijmegen Breakage Syndrome Obesity Occupational Exposure Operative Surgical Procedures Patients Performance Pharmaceutical Preparations Pharmacotherapy Phase Phenotype Physiological Platinum Play Population Population Study Race Rattus Reporting Reproducibility Research Research Personnel Reticulocytes Rodent Rodent Model Role Sampling Scientist Sickle Cell Anemia Small Business Innovation Research Grant Source Specimen TP53 gene Testing Validation Work arm base chemotherapy epidemiology study genotoxicity human DNA damage human disease human study hydroxyurea lifestyle factors mouse model multidisciplinary next generation patient population phase 2 study population based prototype response sex specific biomarkers temozolomide tool

项目摘要

Project Summary This project will validate two high throughput human blood-based DNA damage assays and develop them into commercial kits. The assays monitor types of damage associated with important human diseases. Whereas the PIG-A assay reports on gene mutation, the micronucleated reticulocyte (MN-RET) assay is responsive to chromosomal breaks and/or losses. The biomarkers are applicable to both humans and laboratory animals and will fulfill two important needs: extension of findings in laboratory animal models to direct studies in humans, and performance of well-controlled mechanistic laboratory studies to understand observations first made in humans. The assays utilize immunomagnetic separation prior to flow cytometry to dramatically enrich the relevant cell populations and thereby enhance assay precision and sensitivity. By providing simple-to-use kits with thoroughly documented reproducibility and inter-laboratory transferability, and validating the biomarkers for specific uses, researchers will have available tools with unprecedented efficiencies for comprehensively studying those factors that contribute to inter-individual differences in human DNA damage. Applications include the study of drug treatments, host and/or life-style factors that contribute to inter-individual differences in DNA damage and repair, exaggerated sensitivities to anti- neoplastic therapies, and population-based epidemiology studies of environmental exposures, including occupational exposures. The project benefits from a strong multidisciplinary team of internationally recognized scientists with a proven track record of successfully converting research advances into reliable commercial assay kits.

项目总结

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Assessment of systemic genetic damage in pediatric inflammatory bowel disease.

DOI：
10.1002/em.22403
发表时间：
2020-11
期刊：
Environmental and molecular mutagenesis
影响因子：
2.8
作者：
Baig A;Avlasevich SL;Torous DK;Bemis JC;Saubermann LJ;Lovell DP;MacGregor JT;Dertinger SD
通讯作者：
Dertinger SD

Human blood PIG-A mutation and micronucleated reticulocyte flow cytometric assays: Method optimization and evaluation of intra- and inter-subject variation.

DOI：
10.1002/em.22393
发表时间：
2020-10
期刊：
Environmental and molecular mutagenesis
影响因子：
2.8
作者：
Torous DK;Avlasevich SL;Khattab MG;Baig A;Saubermann LJ;Chen Y;Bemis JC;Lovell DP;Walker VE;MacGregor JT;Dertinger SD
通讯作者：
Dertinger SD

Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup.

DOI：
10.1002/em.22427
发表时间：
2021-03
期刊：
Environmental and molecular mutagenesis
影响因子：
2.8
作者：
Dertinger SD;Bhalli JA;Roberts DJ;Stankowski LF Jr;Gollapudi BB;Lovell DP;Recio L;Kimoto T;Miura D;Heflich RH
通讯作者：
Heflich RH

Lack of hydroxyurea-associated mutagenesis in pediatric sickle cell disease patients.

儿童镰状细胞病患者缺乏羟基脲相关诱变。

DOI：
10.1002/em.22536
发表时间：
2023
期刊：
Environmental and molecular mutagenesis
影响因子：
2.8
作者：
Torous,DorotheaK;Avlasevich,Svetlana;Bemis,JeffreyC;Howard,Thad;Ware,RussellE;Fung,Chunkit;Chen,Yuhchyau;Sahsrabudhe,Deepak;MacGregor,JamesT;Dertinger,StephenD
通讯作者：
Dertinger,StephenD