Timing Endometrial Receptivity

摘要 核仁通道系统(NCSs)是1960年由电子显微镜(EM)发现的 可以说是人类细胞中最独特的细胞器，由正常情况下的膜小管形成 无膜核。它们是人类子宫内膜上皮细胞(EECS)所特有的，并且不存在 来自任何其他组织或物种。事实上，它们甚至不存在于狒狒的子宫内膜中。它们形成了 每个月只有几天是短暂的，这似乎标志着子宫内膜 是接受胚胎植入的，即所谓的植入窗口(WOI)。虽然术语WOI 虽然它在生殖专家中根深蒂固，但它的定义还远未明确。准确的年代测定 WOI对于辅助生殖技术(ART)，特别是对胚胎的计时是非常重要的 移植(ET)以提高体外受精(IVF)周期的成功率。理想情况下，冷冻的时机- 解冻胚胎移植(FET)将依赖于在移植前确定最大子宫内膜容受性 而不会干扰胚胎植入。然而，目前所有评估子宫内膜的方法 容受性依赖于子宫内膜活检，这已被证明既能预防又能促进 植入。因此，大多数不孕不育专家都会非常犹豫地进行这样的检查 ET当天的侵入性手术。相反，我们开发了一种非侵入性的方法来界定 子宫分泌物脱落细胞中的NCS窗口。我们现在建议利用我们的新方法来 通过重复采样确定各个周期中的NCS窗口，并将其作为诊断进行验证 最大限度提高子宫内膜容受性的工具。我们的建议将为将FET带电时间安排到 提高试管受精周期的成功率。

ABSTRACT Discovered in 1960 by electron microscopy (EM), nucleolar channel systems (NCSs) are arguably the most unique organelles of human cells, formed by membrane tubules in the normally membrane-free nucleus. They are specific to human endometrial epithelial cells (EECs) and absent from any other tissue or species. In fact, they are not even present in baboon endometrium. They form transiently each month only during a few days, which appear to mark the period when the endometrium is receptive to embryo implantation, the so-called window of implantation (WOI). Although the term WOI is firmly established among reproductive specialists, its definition is far from clear. Accurate dating of the WOI is important for assisted reproductive technologies (ART), especially for timing of embryo transfer (ET) to enhance success rates of in vitro fertilization (IVF) cycles. Ideally, timing of frozen- thawed ET (FET) would rely on ascertaining maximal endometrial receptivity right before transfer without interfering with embryo implantation. However, all current methods assessing endometrial receptivity rely on endometrial biopsy, which has been documented to both prevent and promote implantation. Consequently, most infertility specialists would be more than hesitant to perform such an invasive procedure on the day of ET. In contrast, we developed a noninvasive method to delimit the NCS window in exfoliated cells of uterine secretions. We now propose to exploit our novel approach to determine the NCS window in individual cycles by repeated sampling and to validate it as a diagnostic tool for maximal endometrial receptivity. Our proposal will lay the groundwork for timing FET live to boost success rates of IVF cycles.