Timing Endometrial Receptivity

子宫内膜容受性时机

• 批准号: 9601070
• 负责人: U THOMAS MEIER
• 金额: $ 4.75万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-30 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9601070
• 关键词: Assisted Reproductive Technology; Benchmarking; Biopsy; Cell Nucleus; Cells; Data; Diagnostic; Electron Microscopy; Electrons; Embryo Transfer; Endometrial; Endometrium; Epithelial Cells; Epithelium; Fertility; Fertilization in Vitro; Freezing; Future; Histologic; Human; Individual; Infertility; Inflammation; Length; Measures; Membrane; Menstrual cycle; Methods; Molecular; Organelles; Papio; Patients; Persons; Procedures; Randomized Controlled Trials; Sampling; Specialist; System; Testing; Time; Tissues; Variant; Woman; implantation; minimally invasive; molecular marker; natural Blastocyst Implantation; novel strategies; prevent; reproductive; success; tool; transmission process

ABSTRACT Discovered in 1960 by electron microscopy (EM), nucleolar channel systems (NCSs) are arguably the most unique organelles of human cells, formed by membrane tubules in the normally membrane-free nucleus. They are specific to human endometrial epithelial cells (EECs) and absent from any other tissue or species. In fact, they are not even present in baboon endometrium. They form transiently each month only during a few days, which appear to mark the period when the endometrium is receptive to embryo implantation, the so-called window of implantation (WOI). Although the term WOI is firmly established among reproductive specialists, its definition is far from clear. Accurate dating of the WOI is important for assisted reproductive technologies (ART), especially for timing of embryo transfer (ET) to enhance success rates of in vitro fertilization (IVF) cycles. Ideally, timing of frozen- thawed ET (FET) would rely on ascertaining maximal endometrial receptivity right before transfer without interfering with embryo implantation. However, all current methods assessing endometrial receptivity rely on endometrial biopsy, which has been documented to both prevent and promote implantation. Consequently, most infertility specialists would be more than hesitant to perform such an invasive procedure on the day of ET. In contrast, we developed a noninvasive method to delimit the NCS window in exfoliated cells of uterine secretions. We now propose to exploit our novel approach to determine the NCS window in individual cycles by repeated sampling and to validate it as a diagnostic tool for maximal endometrial receptivity. Our proposal will lay the groundwork for timing FET live to boost success rates of IVF cycles.

摘要 1960年，电子显微镜（EM）发现了核仁通道系统（NCS）。 可以说是人类细胞中最独特的细胞器，由正常细胞中的膜小管形成。 无膜核它们特异于人子宫内膜上皮细胞（EECs）， 任何其他组织或物种。事实上，它们甚至不存在于狒狒的子宫内膜中。它们形成 每个月只在几天内短暂出现，这似乎标志着子宫内膜 胚胎着床窗口（Window of Implantation，WOI）。虽然术语WOI 在生殖专家中已牢固确立，但其定义远不明确。准确的年代测定 WOI对于辅助生殖技术（ART）非常重要，尤其是对于胚胎的时间安排 移植（ET），以提高体外受精（IVF）周期的成功率。理想的情况是，冻结的时间- 解冻ET（FET）依赖于在移植前确定最大子宫内膜容受性 而不影响胚胎植入。然而，目前所有评估子宫内膜的方法 接受性依赖于子宫内膜活检，这已被证明可以预防和促进 置入因此，大多数不孕不育专家会犹豫是否要进行这样的检查。 在ET当天进行侵入性手术。相比之下，我们开发了一种非侵入性的方法来界定 子宫分泌物脱落细胞中的NCS窗口。我们现在提出利用我们的新方法， 通过重复采样确定单个周期中的NCS窗口，并将其作为诊断进行验证 最大子宫内膜容受性的工具。我们的建议将奠定基础，定时场效应管生活， 提高IVF周期的成功率。