A nervous system-wide analysis of C. elegans homeobox gene function

线虫同源盒基因功能的神经系统范围分析

• 批准号: 9545254
• 负责人: Oliver Hobert
• 金额: $ 23.41万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9545254
• 关键词: Alleles; Anatomy; Applications Grants; Biological Models; Brain; CRISPR/Cas technology; Caenorhabditis elegans; Complex; Data; Embryo; Evolution; Genes; Genetic; Genetic Transcription; Goals; Homeobox; Homeobox Genes; Individual; Invertebrates; Mediating; Molecular; Nematoda; Nervous system structure; Neurons; Neurotransmitters; Pattern; Proxy; Regulator Genes; Reporter; Reporter Genes; Specific qualifier value; Testing; To specify; base; gene function; homeodomain; loss of function; mutant; nervous system development; neuronal patterning; recruit; tool; transcription factor

PROJECT SUMMARY While common, evolutionarily conserved themes of early neuronal patterning in the developing embryo have emerged over the years, there are, as of now, no common organizational themes for how neuron acquire and maintain their terminally differentiated state. We propose to test here the hypothesis that each neuron class of the nematode C.elegans is uniquely defined – and functionally specified – by a neuron class-specific combination of transcription factors, with at least one of the transcription factors being a homeodomain-type transcription factor. The finding that homeodomain transcription factors control the identity of all neurons in a simple nervous system would have wide- ranging implications for our understanding of evolution of the nervous system and may provide means to classify (and then functionally study) neuron classes in mammalian nervous systems.

项目摘要 虽然在发育过程中，早期神经元模式化的共同的、进化上保守的主题， 胚胎已经出现了多年来，有，截至目前，没有共同的组织主题，如何 神经元获得并维持其终末分化状态。我们建议在这里测试 假设线虫的每个神经元类别都是唯一定义的-功能上 - 由转录因子的神经元类特异性组合指定，其中至少一种转录因子是神经元类特异性的。 转录因子是同源异型域型转录因子。发现同源结构域 在简单的神经系统中，转录因子控制着所有神经元的身份， 对我们理解神经系统的进化具有广泛的意义，并可能提供 这意味着分类（然后功能研究）哺乳动物神经系统中的神经元类别。