The role of the intracellular complement system - the complosome - in monocytes

细胞内补体系统（复合体）在单核细胞中的作用

• 批准号: 9792191
• 负责人: Claudia Kemper
• 金额: $ 15.97万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9792191
• 关键词: Arteries; Biological; Cardiovascular Diseases; Cells; Complement; Data; Event; Eye; Future; Goals; Human; In Vitro; Inflammasome; Intervention; Laboratories; London; Medical; Mitochondria; Molecular; Pathway interactions; Permeability; Pre-Clinical Model; Production; Role; Severities; T-Lymphocyte; Testing; United States National Institutes of Health; complement system; in vivo; inhibitor/antagonist; macrophage; monocyte; mouse model; novel; preclinical development

We have recently (February 2017) moved laboratories from London to the NIH. While working here, we have obtained data defining the pathways by which intracellular C5 is activated in human T cells and monocytes and have shown that this intracellular C5 activation is critical to IL-1b production by T cells/monocytes. We are currently assessing the in vivo biological significance of this observation using appropriate CVD mouse models. We are also testing the effects of cell-permeable C5 inhibitors in vitro with an eye on the development of preclinical models.

我们最近（2017年2月）将实验室从伦敦搬到了NIH。在这里工作时，我们已经获得了定义细胞内C5在人T细胞和单核细胞中被激活的途径的数据，并且已经表明这种细胞内C5激活对T细胞/单核细胞产生IL-1b至关重要。我们目前正在使用适当的CVD小鼠模型评估这一观察结果的体内生物学意义。我们还在体外测试细胞渗透性C5抑制剂的作用，着眼于临床前模型的开发。