ShEEP Request for Peggy Sue by Bio-Techne

ShEEP 请求 Bio-Techne 提供 Peggy Sue

• 批准号: 9906453
• 负责人: Robert Hal Scofield
• 金额: --
• 依托单位: OKLAHOMA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906453
• 关键词: Affect; Aneuploidy; Area; Autoimmune Diseases; Autoimmunity; Award; B-Lymphocytes; Blood; Brain; Cartilage; Cell Nucleus; Cells; Cellular biology; Charge; Chronic; Chronic stress; Cities; Communities; Core Facility; Custom; Degenerative polyarthritis; Dendritic Cells; Detection; Disease; Dose; Endocrinology; Fatty acid glycerol esters; Female; Foundations; Funding; Gene Expression; Gene Proteins; Genes; Genetic; Grant; Health; Health Sciences; Healthcare Systems; Human; Immune; Immunologics; Inflammation; Institution; Interferons; K-Series Research Career Programs; Knee joint; Leadership; Maintenance; Measures; Mediating; Medical Research; Medical center; Meniscus structure of joint; Methodology; Minor; Mus; Oklahoma; Pain; Pathogenesis; Peer Review; Performance; Phenotype; Phosphoproteins; Physiological; Population; Process; Production; Protein Analysis; Proteins; Protocols documentation; Regulation; Research; Research Personnel; Resources; Role; Running; Salivary Glands; Sample Size; Sampling; Sampling Studies; Scientist; Seminal; Sex Bias; Sheep; Signal Transduction; Sjogren' s Syndrome; Surveys; Systemic Lupus Erythematosus; TLR7 gene; Technology; Tissue Sample; Tissues; United States National Institutes of Health; Universities; Veterans; Woman; X Chromosome; X Inactivation; age related; automated analysis; base; career; cell population study; cytokine; flexibility; instrument; male; men; monocyte; protein expression; systemic autoimmune disease; tool

Summary We are requesting monies for the purchase of Peggy Sue instrument from Bio-Techne. This instrument allows researchers to use preprogrammed or custom protocols for automated analysis for the separation, quantitation, identification of nanogram amounts of proteins, including phosphoproteins, derived from a variety of tissues. Peggy Sue is Protein Simple’s top of the line instrument and can do both size as well as charge separation for multiple targets in up to 96 sample in one run. Neither the Oklahoma City VA Health Care System nor the affiliated University of Oklahoma Health Sciences Center have such an instrument that enables high precision, automated analysis of protein characterization from cells or tissues. Therefore, this is a truly unique and valuable resource for the scientific community at the Oklahoma City VA Health Care System. Peggy Sue will be used by seven investigators with 6 Merit grants and 1 Career Development Award. Peggy Sue will have a broad impact on several areas of research important to the health of Veterans, including quantification and characterization of proteins from immune cell subsets of systemic lupus erythematosus and Sjögren’s subjects with or without chromosomal aneuploidies; accurately and sensitively measuring changes in protein content from very small amounts of mouse osteoarthritic knee joint tissues such as the infra-patellar fat pad, meniscus, and cartilage; measuring changes in protein expression from micropunches of discrete brain nuclei following experimental manipulation for investigating chronic stress-induced pain and; quantifying necroptosis in tissues of mice to measure the levels of phosphorylated and non-phosphorylated proteins involved in age-related increase in chronic inflammation.

摘要 我们要求从生物技术公司购买Peggy Sue仪器的资金。这台仪器允许 研究人员使用预编程或定制协议进行自动化分析，以进行分离、定量、 鉴定纳克量的蛋白质，包括来自各种组织的磷蛋白。 Peggy Sue是Protein Simple的顶级仪器，可以同时进行尺寸和电荷分离 一次最多可采集96个样品中的多个目标。俄克拉荷马城退伍军人医疗保健系统和 俄克拉荷马大学附属健康科学中心有这样一种仪器，可以实现高精度， 对细胞或组织中蛋白质特征的自动分析。因此，这是一个真正独一无二的 俄克拉荷马城退伍军人医疗保健系统科学界的宝贵资源。佩吉·苏将 将由7名调查人员使用，获得6项功绩奖励和1项职业发展奖。佩姬·苏将会有一个 对几个对退伍军人健康很重要的研究领域产生广泛影响，包括量化和 系统性红斑狼疮和Sjögren患者免疫细胞亚群蛋白质的特性 有或没有染色体非整倍体；准确和灵敏地测量蛋白质含量的变化 取自极少量的小鼠膝骨性关节炎组织，如膝下脂肪垫、半月板、 和软骨；测量以下不同脑核微穿孔中蛋白质表达的变化 研究慢性应激性疼痛和量化组织中的坏死性下垂的实验操作 以测量与年龄相关的磷酸化和非磷酸化蛋白的水平 慢性炎症增加。