Enhancing CRISPR-Cas for disease modeling in Xenopus
增强 CRISPR-Cas 在非洲爪蟾疾病模型中的应用
基本信息
- 批准号:9900078
- 负责人:
- 金额:$ 20.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2021-03-31
- 项目状态:已结题
- 来源:
- 关键词:Animal ModelAnimalsArchitectureAreaAutomobile DrivingBacteriaBiological ModelsBloodCRISPR/Cas technologyCell modelCellular biologyChromosomesClustered Regularly Interspaced Short Palindromic RepeatsConsumptionDevelopmentDiseaseDisease modelExonsGenerationsGenesGenomic DNAGenomicsLabelLibrariesLimulusLoligoMediatingMessenger RNAMethodsModelingMutationProteinsRefractoryResourcesSpecificityStreptococcus pyogenesSystemTechniquesTemperatureTestingTimeVariantXenopusXenopus laeviscase-by-case basiscold temperatureexperiencegenome editinghuman diseasehuman modelin vivoinnovationmicrobialnon-geneticnovel strategiesprecise genome editing
项目摘要
PROJECT SUMMARY
The recent development of the CRISPR-Cas system has transformed many non-genetic models of cellular
biology, yet the full application of its genome editing capabilities has been largely restricted to warm-blooded
species and to genes which are easily amenable to current methods of CRISPR-Cas mediated gene
disruption. Several species which live at lower temperatures, such as Xenopus and the marine models Loligo
and Limulus, are ideal systems to model human disease, yet current methods of precise genome editing in
these species are inadequate. Furthermore, due to their genomic architecture, many highly-conserved
disease-causing genes are refractory to current methods of genome editing. We propose to expand the
CRISPR-Cas mediated genome editing toolkit in the field of human disease modeling by driving innovation in
two areas: 1) the development of a low-temperature CRISPR-Cas system capable of effecting targeted gene
insertion in model species which thrive at lower temperatures, and 2) to develop a nascent system of targeted
gene disruption by the application of CRISPR-Cas libraries.
项目摘要
CRISPR-Cas系统的最新发展已经改变了许多非遗传细胞模型,
生物学,但其基因组编辑能力的全面应用在很大程度上仅限于温血动物
物种和容易适用于CRISPR-Cas介导的基因重组的当前方法的基因
破坏几种生活在较低温度下的物种,如非洲爪蟾和海洋模型Loligo
和Limulus,是模拟人类疾病的理想系统,但目前精确的基因组编辑方法,
这些物种是不够的。此外,由于它们的基因组结构,许多高度保守的
致病基因对目前的基因组编辑方法是难治的。我们建议扩大
CRISPR-Cas介导的基因组编辑工具包在人类疾病建模领域通过推动创新,
两个领域:1)开发能够影响靶基因的低温CRISPR-Cas系统,
插入在较低温度下茁壮成长的模式物种,以及2)开发靶向的新生系统
通过应用CRISPR-Cas文库进行基因破坏。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('Marko E Horb', 18)}}的其他基金
Xenopus models of human disease by targeted genome editing
通过靶向基因组编辑建立人类疾病非洲爪蟾模型
- 批准号:
9257431 - 财政年份:2015
- 资助金额:
$ 20.75万 - 项目类别:
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