XenCAT: Xenopus Single Cell Atlas

XenCAT：非洲爪蟾单细胞图谱

• 批准号: 10807246
• 负责人: Marko E Horb
• 金额: $ 24.88万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10807246
• 关键词: Adopted; Adult; Amphibia; Animal Model; Atlases; Benchmarking; Biological Metamorphosis; Biological Models; Biomedical Research; Brain; Cell Cycle; Cell Nucleus; Cell Size; Cells; Cellular biology; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communication; Communities; Complement; Cyclins; Data; Data Set; Destinations; Development; Disease; Disease model; Dissociation; Embryo; Embryology; Embryonic Development; Evolution; Extracellular Matrix; Funding; Gene Expression Profiling; Genes; Genetic; Genetic Transcription; Heart; Human; Immune system; Individual; Infrastructure; Investigation; Kidney; Learning; Left; Link; Methods; Modeling; Molecular; Mus; Natural regeneration; Neurobiology; Nuclear; Organ; Organism; Organogenesis; Physiological; Physiology; Procedures; Process; Proteins; Proteomics; Protocols documentation; Publishing; Research; Research Personnel; Research Project Grants; Resolution; Rest; Sampling; Science; Signal Transduction; Specialist; Specific qualifier value; System; Time; Tissue atlas; Tissues; United States National Institutes of Health; Work; XenBase; Xenopus; Zebrafish; biological systems; blastomere structure; body system; cell type; combinatorial; data sharing; embryo cell; experience; experimental study; gastrulation; gene interaction; human disease; human genomics; innovation; insight; malformation; model organism; mutant; nuclear reprogramming; single cell analysis; therapeutic evaluation; tool; transcriptomics

Project Summary The genetic causes of human diseases are rapidly being identified thanks to a revolution in human genomics. Progress toward a deeper understanding, however, requires further analysis of the underlying developmental, cellular and molecular mechanisms, as well as the establishment of predictive disease models to test therapeutic options. Ultimately, genes do not function in isolation; they are grouped spatially and temporally at multiple nested levels, the most salient functional unit being the single cell. Observing biological systems at the cellular level provides an unprecedented opportunity to define functional modularity and combinatorial interactions of genes in various physiological contexts. Many of these contexts are conserved in evolution, deviations from which produce important innovations but which also lead to malformations and disease. Accordingly, a Human Cell Atlas is being built with the hope that it will form a core of this single-cell perspective. Parallel work in model organisms will be crucial, and cell atlases are being constructed currently e.g. in mouse and zebrafish. From Gurdon’s discovery of nuclear reprogramming, through characterization of the cyclins that drive the cell cycle, to many recent discoveries on signaling among cells, Xenopus remains at the forefront of biomedical research, as a unique model. We propose to establish a Single Cell Atlas for this important model system which would enhance the value of the unique methods already available in Xenopus and allow effective communication to other experimental systems including human. It will be a critical complement to other emerging Xenopus tools, such as CRISPR-edited mutant lines, which could be most easily characterized in developmental and adult function at the single- cell level. Moreover, the large cell size of amphibian embryonic cells has already made single-cell proteomics possible in Xenopus, well ahead of other organisms; thus, Xenopus is the natural choice for spearheading the shift towards single-cell proteomics. Overall, this project will enhance a critical animal model for the investigation of human disease mechanisms and open new horizons for many already supported NIH projects in other Institutes that focus on specific organ systems and disease.

项目总结