XenCAT: Xenopus Single Cell Atlas
XenCAT:非洲爪蟾单细胞图谱
基本信息
- 批准号:10807246
- 负责人:
- 金额:$ 24.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2026-07-31
- 项目状态:未结题
- 来源:
- 关键词:AdoptedAdultAmphibiaAnimal ModelAtlasesBenchmarkingBiological MetamorphosisBiological ModelsBiomedical ResearchBrainCell CycleCell NucleusCell SizeCellsCellular biologyClustered Regularly Interspaced Short Palindromic RepeatsCollaborationsCommunicationCommunitiesComplementCyclinsDataData SetDestinationsDevelopmentDiseaseDisease modelDissociationEmbryoEmbryologyEmbryonic DevelopmentEvolutionExtracellular MatrixFundingGene Expression ProfilingGenesGeneticGenetic TranscriptionHeartHumanImmune systemIndividualInfrastructureInvestigationKidneyLearningLeftLinkMethodsModelingMolecularMusNatural regenerationNeurobiologyNuclearOrganOrganismOrganogenesisPhysiologicalPhysiologyProceduresProcessProteinsProteomicsProtocols documentationPublishingResearchResearch PersonnelResearch Project GrantsResolutionRestSamplingScienceSignal TransductionSpecialistSpecific qualifier valueSystemTimeTissue atlasTissuesUnited States National Institutes of HealthWorkXenBaseXenopusZebrafishbiological systemsblastomere structurebody systemcell typecombinatorialdata sharingembryo cellexperienceexperimental studygastrulationgene interactionhuman diseasehuman genomicsinnovationinsightmalformationmodel organismmutantnuclear reprogrammingsingle cell analysistherapeutic evaluationtooltranscriptomics
项目摘要
Project Summary
The genetic causes of human diseases are rapidly being identified thanks to a revolution in
human genomics. Progress toward a deeper understanding, however, requires further analysis
of the underlying developmental, cellular and molecular mechanisms, as well as the
establishment of predictive disease models to test therapeutic options. Ultimately, genes do not
function in isolation; they are grouped spatially and temporally at multiple nested levels, the
most salient functional unit being the single cell. Observing biological systems at the cellular
level provides an unprecedented opportunity to define functional modularity and combinatorial
interactions of genes in various physiological contexts. Many of these contexts are conserved in
evolution, deviations from which produce important innovations but which also lead to
malformations and disease. Accordingly, a Human Cell Atlas is being built with the hope that it
will form a core of this single-cell perspective. Parallel work in model organisms will be crucial,
and cell atlases are being constructed currently e.g. in mouse and zebrafish. From Gurdon’s
discovery of nuclear reprogramming, through characterization of the cyclins that drive the cell
cycle, to many recent discoveries on signaling among cells, Xenopus remains at the forefront of
biomedical research, as a unique model. We propose to establish a Single Cell Atlas for this
important model system which would enhance the value of the unique methods already available
in Xenopus and allow effective communication to other experimental systems including human.
It will be a critical complement to other emerging Xenopus tools, such as CRISPR-edited mutant
lines, which could be most easily characterized in developmental and adult function at the single-
cell level. Moreover, the large cell size of amphibian embryonic cells has already made single-cell
proteomics possible in Xenopus, well ahead of other organisms; thus, Xenopus is the natural
choice for spearheading the shift towards single-cell proteomics. Overall, this project will
enhance a critical animal model for the investigation of human disease mechanisms and open
new horizons for many already supported NIH projects in other Institutes that focus on specific
organ systems and disease.
项目摘要
由于遗传学的革命,人类疾病的遗传原因正在迅速被确定
人类基因组学然而,要想取得更深入的了解,还需要进一步的分析
潜在的发育,细胞和分子机制,以及
建立预测疾病模型以测试治疗方案。最终,基因不会
它们在空间和时间上按多个嵌套级别分组,
最显著的功能单位是单个细胞。在细胞中观察生物系统
级别提供了前所未有的机会,定义功能模块化和组合
基因在各种生理环境中的相互作用。其中许多上下文都保存在
进化,偏离它产生重要的创新,但也导致
畸形和疾病。因此,正在建立一个人类细胞图谱,希望它
将成为单细胞理论的核心。在模式生物中的平行工作将是至关重要的,
并且目前正在例如小鼠和斑马鱼中构建细胞图谱。从古尔登的
发现核重编程,通过表征驱动细胞的细胞周期蛋白
周期,许多最近发现的细胞之间的信号,非洲爪蟾仍然处于最前沿,
生物医学研究,作为一个独特的模式。我们建议为此建立一个单细胞图谱
一个重要的示范系统,将提高现有独特方法的价值
并允许与包括人类在内的其他实验系统进行有效的通信。
它将成为其他新兴非洲爪蟾工具的重要补充,例如CRISPR编辑的突变体,
线,这可能是最容易的特点,在发展和成人的功能,在单一的-
细胞水平。此外,两栖动物胚胎细胞的细胞尺寸大,已经制成单细胞
蛋白质组学可能在非洲爪蟾,远远领先于其他生物;因此,非洲爪蟾是自然的
是引领单细胞蛋白质组学转变的首选。总体而言,该项目将
加强关键动物模型的研究人类疾病的机制和开放
新的视野,许多已经支持的NIH项目在其他研究所,重点是具体的
器官系统和疾病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Marko E Horb', 18)}}的其他基金
Enhancing CRISPR-Cas for disease modeling in Xenopus
增强 CRISPR-Cas 在非洲爪蟾疾病模型中的应用
- 批准号:
9900078 - 财政年份:2019
- 资助金额:
$ 24.88万 - 项目类别:
Xenopus models of human disease by targeted genome editing
通过靶向基因组编辑建立人类疾病非洲爪蟾模型
- 批准号:
9257431 - 财政年份:2015
- 资助金额:
$ 24.88万 - 项目类别:
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