A biomarker panel based smart mini-array system for the homecare of autoimmune kidney diseases

项目摘要 系统性红斑狼疮（SLE）是一种复杂的自身免疫性疾病，主要影响 妇女狼疮性肾炎（LN）是导致高死亡率的主要原因。及时治疗LN发作 使用抗炎和免疫抑制药物是至关重要的，但目前还没有 在床旁或家庭环境中诊断; LN发作诊断的金标准是肾 活检在家中或床旁（POC）检测LN发作将改善治疗 响应性访问和促进SLE临床研究。我们提出三个具体目标：目标1： 目的：建立尿系统性红斑狼疮（SLE）发作标志物的多基因定量微阵列（BMA）。 目的2：通过建立一种可靠、方便的生物标志物微阵列， 智能手机分析和报告系统（SBARS）。目的3：评价 BMA-SBARS测试在疾病环境中的性能，使用来自交叉 LN患者的截面和纵向队列。拟议工作的主要创新之处 在于其开发了用于LN发作的原型实用POC/自我检测尿液测试。的 技术本身将对家庭慢性病自我管理产生广泛影响， 特别是对于老年患者。

Project summary Systemic lupus erythematosus (SLE) is a complex autoimmune disease predominantly affecting women. Lupus nephritis (LN), is a leading cause of high mortality. Timely treatment of LN flares with anti-inflammatory and immunosuppressant drugs is critical, but flares are not currently diagnosed in a point-of-care or home setting; the gold standard for LN flare diagnosis is renal biopsy. At-home or Point-of-care (POC) detection of LN flares would improve treatment responsiveness access and facilitate SLE clinical research. We propose 3 specific aims: AIM 1: To develop multiplex quantitative Biomarker Mini-array (BMA) for urinary SLE flare markers. AIM 2: To develop a reliable, convenient Biomarker Mini-array for LN flare identification by smartphone based analysis and reporting system (SBARS). AIM 3: To evaluate the clinical performance of the BMA-SBARS tests in disease settings, using urine samples from cross- sectional and longitudinal cohorts of LN patients. The primary innovation of the proposed work lies in its development of a prototype practical POC/self-testing urine test for LN flares. The technology itself will have broad impact in self-management of chronic diseases at home, especially for aged patients.