Impacts of Urbanization on Vector Biology and Transmission of Dengue in China

项目摘要 标题：城市化对病媒生物学和 登革热在中国的传播 登革热是一种由登革热病毒引起的急性传染病， 由伊蚊传播。尽管中国有严格的控制计划， 登革热最近在中国南方城市地区爆发。控制 减少蚊子数量是预防登革热的唯一可行策略， 目前还没有治疗登革热的有效药物。化学杀虫剂的使用代表了 这是中国和全世界登革热病媒控制的最重要因素。我们 初步数据表明， 中国南方的城市。这种竞争性的续期申请将测试 中心假设是登革热的频繁爆发和杀虫剂的大量使用 导致杀虫剂的出现和传播 登革热病媒的抗药性，从而增加病媒种群 密度和登革热病媒能力。我们提出了三个相互关联的具体 目的：1）了解城乡登革热病媒人群的抗药性水平 环境，2）开发用于杀虫剂抗性监测的新分子工具 通过更好地了解耐药机制， 监测城市和农村环境中的杀虫剂抗药性，以及3） 确定杀虫剂抗药性对蚊子存活率、病媒 城市和农村环境中的能力和矢量能力。我们将专注于 白纹伊蚊是一种高度入侵的物种，也是中国最重要的登革热病媒。 华南该项目的知识和工具将有助于指导 选择用于登革热病媒控制的杀虫剂，促进杀虫剂抗药性 加强对登革热流行病学的认识 在城市环境中，杀虫剂的抗药性正在迅速增加。

Project Summary Title: Impacts of Urbanization on Vector Biology and Transmission of Dengue in China Dengue fever is an acute infectious disease caused by the dengue virus and transmitted by Aedes mosquitoes. Despite the intense control program in China, major dengue outbreaks have recently occurred in the urban area of Southern China. Control of mosquito populations is the only available strategy for prevention of dengue because there are no effective drugs for dengue. The use of chemical insecticides represents the most important element of vector control for dengue in China and worldwide. Our preliminary data indicates a rapid rise of pyrethroid resistance in the dengue vectors in urban areas of southern China. This competing renewal application will test the central hypothesis that frequent dengue outbreaks and intensive insecticide use in the urban environment lead to an emergence and spread of insecticide resistance in the dengue vector, which in turn increases vector population density and vectorial capacity for dengue. We proposed three interlinked specific aims: 1) to determine resistance level of dengue vector populations in urban and rural environments, 2) to develop new molecular tools for insecticide resistance surveillance through a better understanding of resistance mechanisms, and to conduct molecular surveillance of insecticide resistance in urban and rural environments, and 3) to determine the effect of insecticide resistance on mosquito survivorship, vector competence and vectorial capacity in urban and rural environments. We will focus on Aedes albopictus, a highly invasive species and the most important dengue vector in Southern China. The knowledge and tools from this project will help in guiding the selection of insecticides for dengue vector control, facilitate insecticide resistance surveillance and management, and enhance our understanding of dengue epidemiology in the urban environment where insecticide resistance is rapidly increasing.