Protein Core

项目摘要 蛋白质核心的总体目标是首先提供原子分辨率、结构和功能方面的洞察 淋病奈瑟菌蛋白抗原的设计与工程。第二个目标是 高效、高效地生产优质、大量的抗原。我们的目标是提供高- 使用X射线结晶学和/或冷冻电子显微镜解析结构信息，我们计划 使用我们最近发现的SLAM蛋白质分泌系统表达和纯化大量纯蛋白质。 革兰氏阴性细菌病原体外膜表面的蛋白质提供了一个有价值的靶点 用于产生抗微生物治疗药物和预防性药物。在病原体中，许多外膜 蛋白质是获取营养或逃避宿主免疫所必需的，因此对宿主来说是必不可少的。 殖民时期或侵袭性疾病期间。在细菌细胞表面，这些外膜蛋白提供 表面可获得的表位，可作为疫苗实施中的抗原 病原体--因此，对病原体结构和功能的了解有助于深入了解病原体的发育。 针对革兰氏阴性病原体的新疗法。 莫赖斯博士的计划愿景是提供一种开发和生产新的疫苗抗原和一种新的 疫苗生产系统。莫赖斯博士可用的微生物学工具、仪器和基础设施 他的合作者让他在结构生物学方面做出了多产和成功的贡献 膜蛋白尤其与细菌致病机制和疫苗设计有关。

Project Summary The overall goal of the Protein Core is to first provide atomic resolution structural and functional insight into the design and engineering of the proposed protein antigens from Neisseria gonorrhoeae. The second aim is to produce the antigens with high quality and quantity in an efficient and effective manner. We aim to provide high- resolution structural information using X-ray Crystallography and/or Cryo-Electron Microscopy and we plan to express and purify large quantities of pure protein using our recently discovered SLAM protein secretion system. Proteins residing the outer membrane surface of Gram-negative bacterial pathogens provide a valuable target for the generation of antimicrobial therapeutics and prophylactics. In pathogens, many of the outer membrane proteins are required for either nutrient acquisition or host immune evasion and therefore are essential for host colonization or during invasive disease. At the bacterial cell surface, these outer membrane proteins provide surface accessible epitopes that can serve as antigens in the implementation of vaccines against these pathogens—thus an understanding of their structure and function provides useful insights into the development of new therapeutics against Gram-negative pathogens. Dr. Moraes' program vision is to provide an avenue to develop and produce new vaccine antigens and a novel vaccine production system. The microbiological tools, instrumentation and infrastructure available to Dr. Moraes and his collaborators allow him to be productive and successful at contributing to the structural biology of membrane proteins particularly as is relates to bacterial pathogenesis and vaccine design.