Omics and Bioinformatics Facility Core

摘要（组学和生物信息学核心） 下一代测序的应用，靶向组学测定的方案，以及 所有公开的组学数据集都在继续快速扩展。因此，很难 环境健康科学家保持最新的所有技术，分析方法，并提供 与他们的研究相关的数据。组学和生物信息学核心（OBIC）的目标是提供 为密歇根州生命阶段环境中心的研究人员提供全面和创新的支持 用于研究设计、样品制备、分析、解释和 综合各种基于组学的研究，并就现有的机会对他们进行教育。OBIC 简化了组学研究的整个过程，使中心的研究人员能够投入更多的精力， 资源来回答困难的环境健康科学问题。生命周期环境暴露 可能会通过多种分子机制，包括表观遗传， 遗传学、转录组学和代谢组学机制。我们提供专业知识的技术， 分析包括焦磷酸测序和Sequenom，RNA-seq，基因表达和DNA甲基化 微阵列，microRNA表达，人类甲基化450微珠芯片，还原亚硫酸氢盐 测序（RRBS）和MeDIP-seq，和ChIP-seq。OBIC的目标将通过四个方面来实现 明确的目标。首先，我们将为M-LEEaD调查人员提供设计和实施 广泛的组学和基因靶向研究（例如基因组学、表观基因组学、蛋白质组学、代谢组学）， 制定方案，包括技术平台的选择以及样品的收集和制备。 其次，我们将为M-LEEaD研究者提供广泛的生物信息学分析服务， 包括标准或自定义分析、数据管理和可视化支持以及路径/网络 分析。第三，OBIC通过开发新的生物信息学方法和工具来支持社区 以及与技术进步保持同步的新技术协议。最后，OBIC教育 中心的教师，工作人员和学生的组学分析和生物信息学方法最相关的环境 健康科学研究，并连接环境科学家与潜在的合作者在组学和 生物信息学M-LEEaD研究者受益于免费和/或降低成本的服务。我们的服务包括 在赠款和手稿准备方面提供援助，就工具和资源的使用进行个人培训，以及 针对M-LEEaD研究者的生物信息学/组学研讨会。

ABSTRACT (Omics and Bioinformatics Core) Applications of next generation sequencing, protocols for targeted omics assays, and the number and breadth of publicly-available omics datasets all continue to expand at a rapid pace. As such, it is difficult for environmental health scientists to stay up-to-date on all of the technologies, analysis methods, and available data relevant to their research. The objective of the Omics & BioInformatics Core (OBIC) is to provide comprehensive and innovative support to investigators in the Michigan Center on Lifestage Environmental Exposures and Disease (M-LEEaD) for the study design, sample preparation, analysis, interpretation, and integration of a broad range of omics-based studies, and to educate them on available opportunities. OBIC streamlines the entire process of an omics study, allowing center investigators to dedicate more of their own resources to answering difficult environmental health science questions. Lifestage environmental exposures may increase the risk of later disease through a variety of molecular mechanisms, including epigenetic, genetic, transcriptomic, and metabolomics mechanisms. Technologies for which we provide expertise and analysis include pyrosequencing and Sequenom, RNA-seq, gene expression and DNA methylation microarrays, microRNA expression, HumanMethylation450 BeadChip, reduced representation bisulfite sequencing (RRBS) and MeDIP-seq, and ChIP-seq. The OBIC objective will be accomplished through four specific aims. First, we will provide M-LEEaD investigators expertise in the design and implementation of a broad range of omics and gene target studies (e.g. genomic, epigenomic, proteomic, metabolomic) and development of protocols, including technology platform choice, and sample collection and preparation. Second, we will provide M-LEEaD investigators with a broad-range of bioinformatics analysis services, including standard or custom analyses, support for data management and visualization, and pathway/network analyses. Third, OBIC supports the community through development of novel bioinformatics methods and tools and protocols for novel technologies staying apace with technological advancements. Finally, OBIC educates center faculty, staff, and students on omics assays and bioinformatics methods most relevant to environmental health sciences research, and connects environmental scientists with potential collaborators in omics and bioinformatics. M-LEEaD investigators benefit from free and/or reduced cost services. Our services include assistance in grants and manuscript preparation, individual training on the use of tools and resources, and Bioinformatics/Omics workshops targeted to M-LEEaD investigators.