ISU VDL Instrument Grant

ISU VDL 仪器补助金

• 批准号: 9908688
• 负责人: Scott Louis Radke
• 金额: $ 22.48万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-05 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9908688
• 关键词: ISU; VDL; Instrument; Grant

PROJECT SUMMARY Contamination of food and feed for food producing and companion animals can harm animals directly, but also indirectly harm consumers of chemically contaminated animal food products. In some instances pet and food producing animals can act as sentinels, warning of future human intoxications. For example, in the melamine food outbreak of 2007, detection of melanine in pet food preceded detection of the same in infant formula which sickened and killed children in China. Therefore, early and rapid detection of toxins in animal food/feed can protect public health. The melamine food outbreak of 2007 revealed many deficiencies in veterinary diagnostic laboratory capacity to rapidly detect and quantify food contamination during emergency outbreaks. This led to the founding, 7-8 years ago, of Vet-LIRN, a network of Federal, State, and University Laboratories whose function is to catalyze the development of new and rapid tissue-based analytical techniques, provide for well-equipped and well-staffed facilities, improve the effectiveness of partnerships to coordinate food safety and defense resources to reduce the incidence of foodborne illnesses, and to increase communication among network labs. The Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) is a founding member and an avid supporter and participant in network activities. A lot more remains to be done to protect animal and public health. This FOA is supporting multiple projects, including purchase of equipment needed for testing or developing tests for feed/food-borne toxicants. This project proposal has one Specific Aim: Specific Aim #1: Purchase of one new Thermo Fisher Scientific TSQ Altis UHPLC/triple quadrupole LC/MS instrument. Compared with conventional existing LC/MS, this TSQ Altis UHPLC will provide separations with equivalent chromatographic resolution up to 5-6 times more rapidly. This high throughput capability can be critically important in an emergency response situation. For example, in the event of a pet-food recall event, this equipment will enable our lab to conduct high throughput sample analysis, turning out test results of multiple test samples 5-6 times faster than our existing Waters LC/MS system currently dedicated for Vet-LIRN activities. If awarded, the new system will also allow our lab to turn out regular diagnostic cases much faster. If awarded this will support surge capacity sample testing during large scale animal food/feed emergency events identified by the VPO. Ultimately, the ISU VDL will maintain full functionality as a Vet-LIRN laboratory, with readiness to contribute to the FDA mission in fulfilment of requirements of the Food Safety Modernization Act.

项目摘要 食品生产和伴侣动物的食品和饲料的污染会直接伤害动物， 间接伤害消费者的化学污染的动物食品。在某些情况下，宠物和食物 生产动物可以充当哨兵，警告未来人类中毒。例如，在三聚氰胺 2007年食品爆发，在宠物食品中检测到黑色素，先于在婴儿配方奶粉中检测到黑色素 导致中国儿童患病和死亡。因此，早期和快速检测动物食品/饲料中的毒素 可以保护公众健康。2007年的三聚氰胺食品爆发暴露了兽医的许多缺陷， 诊断实验室能力，以便在紧急情况爆发期间迅速检测和量化食品污染。 这导致了7-8年前成立的Vet-LIRN，一个由联邦、州和大学实验室组成的网络 其功能是促进新的快速组织分析技术的发展， 设备齐全、人员配备齐全的设施，提高合作伙伴关系的有效性，以协调食品安全 和国防资源，以减少食源性疾病的发病率，并增加沟通， 网络实验室爱荷华州州立大学兽医诊断实验室（ISU VDL）是创始成员， 网络活动的热心支持者和参与者。保护动物还有很多工作要做， 公共卫生该FOA支持多个项目，包括购买测试所需的设备， 开发饲料/食源性毒物检测。本项目提案有一个具体目标： 具体目标1：购买一台新的Thermo Fisher Scientific TSQ Altis UHPLC/三重四极杆LC/MS 仪器与传统的现有LC/MS相比，该TSQ Altis UHPLC将提供以下分离： 等效色谱分离度提高5-6倍。这种高吞吐量能力可以是 在紧急情况下至关重要。例如，在宠物食品召回事件中， 该设备将使我们的实验室能够进行高通量的样品分析， 多个测试样品的速度比我们现有的沃茨LC/MS系统快5-6倍，目前专用于Vet-LIRN 活动如果获奖，新系统还将使我们的实验室能够更快地产生常规诊断病例。如果 授予该项目将支持在大规模动物食品/饲料紧急事件期间进行快速增加的样品检测能力 由VPO识别。最终，ISU VDL将保持作为Vet-LIRN实验室的全部功能， 随时准备为FDA的使命做出贡献，以满足《食品安全现代化法案》的要求。