ShEEP Request for Laser Scanning Microscope (LSM) 880

ShEEP 请求激光扫描显微镜 (LSM) 880

• 批准号: 9907067
• 负责人: Wendy B Bollag
• 金额: --
• 依托单位: CHARLIE NORWOOD VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907067
• 关键词: 10 year old; Area; Arrestins; Atherosclerosis; Basic Science; Biological Assay; Biomedical Research; Cell physiology; Cells; Cellular biology; Chronic; Collaborations; Collection; Computer software; Copper; Corneal Injury; Data; Detection; Dimensions; Elements; Ensure; Equipment; Fluorescence Resonance Energy Transfer; Funding; Future; Health; Image; Imagery; Imaging Techniques; Inflammation; Kidney; Laboratories; Lasers; Light; Lighting; Location; Manufacturer Name; Microscope; Microscopy; Microtomy; Mission; Modeling; Nicotinic Acids; Noise; Optics; Organelles; Outcome Measure; Parkinson Disease; Phosphatidylglycerols; Photons; Positioning Attribute; Principal Investigator; Productivity; Proteins; Psoriasis; Research; Research Personnel; Research Project Grants; Resolution; Retinal Diseases; Role; Sampling; Scanning; Science; Scientist; Services; Shapes; Sheep; Signal Transduction; Site; Specimen; Speed; System; Technology; Temperature; Thick; Time; Tissues; Translational Research; Universities; Update; Veterans; Width; Wound Healing; adaptive optics; aquaporin 3; arginase; career; confocal imaging; detector; experimental study; fluorophore; improved; injury and repair; instrument; interest; kidney repair; light emission; muscle invasive bladder cancer; new technology; optical imaging; two-dimensional; two-photon; waiver

The requested instrument is a laser-scanning microscope (LSM model 880), a confocal microscope manufactured by Zeiss, which is needed to replace an approximately 10- year-old Zeiss LSM 510, that is no longer supported by the company because it is outdated. The updated LSM 880 microscope allows high-resolution confocal imaging of multiple fluorophores, permitting visualization of the subcellular location of proteins of interest and/or the co-localization of these proteins with other proteins or cellular organelles. Importantly, the LSM 880 is equipped with AiryScan (Airy Disk) technology; this newer technology employs many single and independent detector elements, with each of these 32 detectors acting like a very small pinhole. The confocal pinhole itself remains open and does not block light; thus, all of the photons from the entire Airy disk are collected. The signals from all of the detector elements are then reassigned to their correct position, producing an image with an increased signal-to-noise ratio (SNR) and enhanced resolution. Thus, the AiryScan allows the collection of all of the emitted light and combines super-resolution with high speed and sensitivity. The LSM 880 can also be upgraded to permit two-photon technology, which would allow confocal imaging of thicker specimens, as well as cell wound healing assays. This instrument will support at least 7 VA Merit-funded research projects from 6 VA Principal Investigators: (1) #CX001357: “Epidermal aquaporin-3 in psoriasis” and #BX004735: “Phosphatidylglycerol to promote corneal wound healing and inhibit chronic inflammation” (PI: Dr. Wendy Bollag); (2) #BX001232: “Role of copper transporters in atherosclerosis” (PI: Dr. Tohru Fukai); (3) #BX001233: “Mechanisms of traumatic retinopathy: Role of arginase” (PI: Dr. Ruth Caldwell); (4) #BX003862: “Role of B- arrestins in muscle-invasive bladder cancer” (PI: Dr. Balakrishna Lokeshwar); (5) #BX000319 “Ischemic kidney injury and kidney repair” (PI: Dr. Zheng Dong; Dr. Dong is also a Senior VA Research Career Scientist); and (6) #RX001613: “GPR109A and Parkinson's disease: Role of niacin in outcome measures” (PI: Dr. Chandramohan Wakade). The Zeiss LSM 880 is a state-of-the-art system with a proven track record, allowing high-resolution optical sections, and it is expected that this shared instrument will promote collaborations between VA Merit-funded investigators and increase research productivity thus advancing the VA mission to improve veterans’ health.

所要求的仪器是激光扫描显微镜（LSM型号880）、共聚焦显微镜（LSM型号880）和激光扫描显微镜（LSM型号880）。 蔡司制造的显微镜，这是需要取代约10- 年的蔡司LSM 510，这是不再由该公司支持，因为它是 过时了更新的LSM 880显微镜允许高分辨率共焦成像， 多个荧光团，允许可视化的蛋白质的亚细胞位置， 感兴趣和/或这些蛋白质与其他蛋白质或细胞的共定位 细胞器重要的是，LSM 880配备了AiryScan（艾里盘）技术; 这种较新的技术采用许多单个和独立的检测器元件， 这32个探测器中的每一个都像一个非常小的针孔。共焦针孔本身 仍然是开放的，不阻挡光线;因此，所有的光子从整个艾里盘 都收集起来了然后，来自所有检测器元件的信号被重新分配给它们的 正确的位置，产生具有增加的信噪比（SNR）的图像，以及 增强分辨率。因此，AiryScan允许收集所有发出的光 并将超分辨率与高速和灵敏度相结合。LSM 880还可以 升级到允许双光子技术，这将允许共焦成像， 更厚的标本，以及细胞伤口愈合测定。该工具将支持 至少7个VA Merit资助的研究项目，来自6个VA主要研究者：（1） #CX001357：“银屑病中的表皮水通道蛋白-3”和#BX004735： “磷脂酰甘油可促进角膜伤口愈合并抑制慢性 （PI：Wendy Bollag博士）;（2）#BX 001232：“铜转运蛋白在炎症中的作用” 动脉粥样硬化”（PI：Tohru Fukai博士）;（3）#BX 001233：“创伤性动脉粥样硬化的机制” 视网膜病变：视网膜酶的作用”（PI：Ruth考德威尔博士）;（4）#BX 003862：“B- 肌肉浸润性膀胱癌中的抑制蛋白”（PI：Balakrishna Lokeshwar博士）;（5） #BX000319“缺血性肾损伤和肾修复”（主要研究者：Dr. Zheng Dong; Dr. Dong是 也是高级VA研究职业科学家）;和（6）#RX 001613：“GPR 109 A和 帕金森病：烟酸在结果测量中的作用”（PI：Chandramohan博士 Wakade）。蔡司LSM 880是一个最先进的系统，具有良好的跟踪记录， 允许高分辨率的光学切片，预计这种共享仪器 将促进VA Merit资助的研究人员之间的合作， 研究生产力，从而推进退伍军人管理局的使命，以改善退伍军人的健康。