ShEEP Request for Laser Scanning Microscope (LSM) 880

所要求的仪器是激光扫描显微镜(LSM 880型)，共焦 蔡司制造的显微镜，需要用来取代大约10- 已有一年历史的蔡司LSM 510不再受公司支持，因为它是 已经过时了。最新的LSM 880显微镜允许高分辨率共聚焦成像 多个荧光团，允许可视化蛋白质的亚细胞位置 感兴趣和/或这些蛋白质与其他蛋白质或细胞的共定位 细胞器。重要的是，LSM 880配备了AiryScan(Ary Disk)技术； 这项较新的技术采用了许多单一和独立的探测器元件， 这32个探测器中的每一个都像一个非常小的针孔。共焦针孔本身 保持开放，不阻挡光线；因此，来自整个艾利盘的所有光子 都被收集起来。然后，来自所有探测器元件的信号被重新分配给它们的 正确的位置，产生具有更高信噪比(SNR)的图像 增强的分辨率。因此，AiryScan允许收集所有发射的光 并将超分辨率与高速度和高灵敏度相结合。LSM 880还可以 升级到允许双光子技术，这将允许共焦成像 更厚的标本，以及细胞伤口愈合分析。这台仪器将支持 来自6名退伍军人事务部首席调查员的至少7个退伍军人事务部立功资助的研究项目：(1) #CX001357：“银屑病中的表皮水通道蛋白-3”和#BX004735： “磷脂酰甘油促进角膜创面愈合，抑制慢性 炎症“(PI：温迪·博拉格博士)；(2)#BX001232：”铜转运蛋白在 动脉粥样硬化“(PI：Tohru Fukai博士)；(3)#BX001233：”创伤的机制“ 视网膜病变：精氨酸酶的作用“(PI：Ruth Caldwell博士)；(4)#BX003862：”B- 肌肉浸润性膀胱癌的止痛药“(PI：Dr.Balakrishna Lokeshwar)；(5) #BX000319《缺血性肾损伤与肾修复》(PI：郑东博士；董博士是 也是退伍军人事务部高级研究职业科学家)；和(6)#RX001613：“Gpr109a和 帕金森氏病：烟酸在结果评估中的作用“(PI：Chandramohan博士 瓦卡德)。蔡司LSM 880是一款最先进的系统，具有良好的跟踪记录， 允许高分辨率光学切片，预计这台共享仪器 将促进退伍军人事务部功勋资助的调查人员之间的合作，并增加 研究生产力，从而推进退伍军人管理局改善退伍军人健康的任务。

The requested instrument is a laser-scanning microscope (LSM model 880), a confocal microscope manufactured by Zeiss, which is needed to replace an approximately 10- year-old Zeiss LSM 510, that is no longer supported by the company because it is outdated. The updated LSM 880 microscope allows high-resolution confocal imaging of multiple fluorophores, permitting visualization of the subcellular location of proteins of interest and/or the co-localization of these proteins with other proteins or cellular organelles. Importantly, the LSM 880 is equipped with AiryScan (Airy Disk) technology; this newer technology employs many single and independent detector elements, with each of these 32 detectors acting like a very small pinhole. The confocal pinhole itself remains open and does not block light; thus, all of the photons from the entire Airy disk are collected. The signals from all of the detector elements are then reassigned to their correct position, producing an image with an increased signal-to-noise ratio (SNR) and enhanced resolution. Thus, the AiryScan allows the collection of all of the emitted light and combines super-resolution with high speed and sensitivity. The LSM 880 can also be upgraded to permit two-photon technology, which would allow confocal imaging of thicker specimens, as well as cell wound healing assays. This instrument will support at least 7 VA Merit-funded research projects from 6 VA Principal Investigators: (1) #CX001357: “Epidermal aquaporin-3 in psoriasis” and #BX004735: “Phosphatidylglycerol to promote corneal wound healing and inhibit chronic inflammation” (PI: Dr. Wendy Bollag); (2) #BX001232: “Role of copper transporters in atherosclerosis” (PI: Dr. Tohru Fukai); (3) #BX001233: “Mechanisms of traumatic retinopathy: Role of arginase” (PI: Dr. Ruth Caldwell); (4) #BX003862: “Role of B- arrestins in muscle-invasive bladder cancer” (PI: Dr. Balakrishna Lokeshwar); (5) #BX000319 “Ischemic kidney injury and kidney repair” (PI: Dr. Zheng Dong; Dr. Dong is also a Senior VA Research Career Scientist); and (6) #RX001613: “GPR109A and Parkinson's disease: Role of niacin in outcome measures” (PI: Dr. Chandramohan Wakade). The Zeiss LSM 880 is a state-of-the-art system with a proven track record, allowing high-resolution optical sections, and it is expected that this shared instrument will promote collaborations between VA Merit-funded investigators and increase research productivity thus advancing the VA mission to improve veterans’ health.