3D-Printed Superhydrophobic-Tipped Optical Fiber for Targeted Periodontal Photodynamic Therapy

项目摘要/摘要 根除牙周袋中的细菌对牙医和 口腔护理专业人士。这个问题很严重；严重的牙周炎是最常见的第六位。 全世界有超过7.43亿人受到影响。生产力损失的全球成本 严重的牙周炎估计为540亿美元/年。抗生素伴结垢和根面平整 (SRP)一直是当前治疗的支柱，但可能导致抗生素的发展- 耐药细菌。光动力疗法(PDT)是一种有益的替代疗法，然而，有 其植入面临的几个挑战包括组织染色和较短的穿透深度 因为染料直接进入牙周袋，而且治疗时间很长 由于缺氧口袋中的氧气不足。因此，迫切需要在以下方面取得进一步进展 牙周细菌根除。 SingletO2 Treeutics公司开发的超疏水光纤PDT(SH-PDT)器件尖端 LLC提供杀菌单线态氧(1O2，分子氧的一种细胞毒性激发态) 直接、可控的时尚。这一装置是对反应性气体精确输送的重大突破 单线态氧杀灭低氧菌治疗牙周病。使用精密成型 和3D打印技术，该设备将感光剂集成到光纤的尖端， 可以无痛地插入牙齿和牙床之间，杀死引起牙周炎的细菌 牙周炎。我们的超疏水设计防止了生物流体与致敏剂之间的接触， 消除污渍。此外，牙周袋的消毒将在一次检查中实现。 由于超疏水系统捕获空气的效率，这将为 否则就是低氧环境。这种方法也不会引起细菌抗药性，它会 对革兰氏阴性和革兰氏阳性细菌同样有效。 在这个第二阶段的STTR项目中，我们建议从两个方面研究SH-PDT设备的有效性。 首先，我们将量化该设备在体外杀死细菌生物膜的能力，该细菌生物膜由 与牙周炎相关的三种不同的细菌菌株。其次，我们将量化 使用Wistar大鼠结扎模型在体内杀死牙周炎的装置。一项试点研究将是 进行以确定适当的剂量，然后进行全面研究以获得统计数据 取得了重大成果。这些研究的成功结果将用于设计人体临床试验。 在未来。

Project Summary/Abstract Eradication of bacteria located in periodontal pockets presents a daunting challenge to dentists and oral care professionals. The problem is significant; severe periodontitis is the 6th most prevalent disease worldwide with over 743 million people affected. The global cost of lost productivity from severe periodontitis is estimated to be $54 billion USD/year. Antibiotics with scaling and root planing (SRP) have been the mainstays of current treatments but may lead to the development of antibiotic- resistant bacteria. Photodynamic therapy (PDT) is a beneficial alternative, however, there are several challenges to its implantation including staining of tissue and a short penetration depth because the dye is introduced directly into the periodontal pocket as well as long treatment times due lack of oxygen in hypoxic pockets. Thus, there is an urgent need for further advances in periodontal bacteria eradication. The superhydrophobic fiber optic PDT (SH-PDT) device tip developed by SingletO2 Therapeutics LLC delivers bactericidal singlet oxygen (1O2, a cytotoxic excited state of molecular oxygen) in a direct, controllable fashion. This device is a major breakthrough for the precise delivery of reactive singlet oxygen to kill hypoxic bacteria for treatment of periodontal diseases. Using precision molding and 3D printing technologies, the device integrates a sensitizer onto the tip of an optical fiber that can painlessly be inserted between the tooth and gum killing bacteria which are the cause of periodontitis. Our superhydrophobic design prevents contact between biofluids and the sensitizer, eliminating staining. Moreover, disinfection of the periodontal pocket will be achieved in a single visit due to the efficiency of the superhydrophobic system to trap air, which will provide oxygen to otherwise hypoxic environments. The approach will also not induce bacterial resistance, and it will be equally effective on gram-negative and gram-positive bacteria. In this Phase II STTR project, we propose to study the efficacy of the SH-PDT device in two ways. First we will quantify the ability of the device to kill a bacterial biofilm in vitro that is comprised of three different bacteria strains associated with periodontitis. Secondly, we will quantify the ability of the device to kill periodontitis in vivo using a Wistar rat ligature model. A pilot study will be conducted to determine the appropriate dose, followed by a full study to acquire statistically significant results. Successful results from these studies will be used to design human clinical trials in the future.