CyTOF platform for the Advanced Cytometry Facility: overcoming fluorescence spectral barriers to truly multiparametric cytometry by mass spectrometry

用于高级细胞计数设施的 CyTOF 平台：克服荧光光谱障碍，通过质谱实现真正的多参数细胞计数

• 批准号: LE140100149
• 负责人: Prof Nicholas King
• 金额: $ 20万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-12-31 至 2015-06-19
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE140100149
• 关键词: CyTOF; platform; Advanced; Cytometry; Facility

Cytometry by time-of-flight mass spectrometry platform for the Advanced Cytometry Facility: overcoming fluorescence spectral barriers to truly multiparametric cytometry by mass spectrometry: This project will provide a flow cytometer capable of analysing single cells by time-of-flight mass spectrometry. Antibody labels for cell components will enable measurement of up to 100 parameters/cell. Developing analytical and modelling algorithms like Spanning tree Progression of Density normalised Events (SPADE), this project will aim to map the relationships of various unelucidated cell lineages, via functional pathway connections. New pathways thus revealed will enable elaboration and use of novel specific molecules in perturbational analyses to confirm and further enhance the understanding of these highly intricate, basic relationships. This will provide unparalleled insight, both into early development of stem cells and mechanisms of maintenance of homeostasis in differentiated cells.

高级细胞计数设施的飞行时间质谱平台细胞计数：通过质谱克服荧光光谱障碍，实现真正的多参数细胞计数：该项目将提供一种能够通过飞行时间质谱分析单细胞的流式细胞仪。用于细胞组分的抗体标记将使得能够测量多达100个参数/细胞。开发分析和建模算法，如生成树密度归一化事件进展（SPADE），该项目旨在通过功能途径连接绘制各种未阐明的细胞谱系的关系。由此揭示的新途径将使在微扰分析中阐述和使用新的特定分子，以确认并进一步增强对这些高度复杂的基本关系的理解。这将为干细胞的早期发育和分化细胞内稳态维持机制提供无与伦比的见解。