Cytometry & Cell Sorting Core

细胞计数法

基本信息

  • 批准号:
    9918400
  • 负责人:
  • 金额:
    $ 14.9万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-02-01 至 2023-01-31
  • 项目状态:
    已结题

项目摘要

Flow cytometry is an important tool in quantifying and phenotyping the cells that define pathways relevant to diabetes. Previously limited to the analysis of circulating immune cells, advances in cell extraction from tissues has allowed flow cytometry to expand its capabilities to the study of developmental, metabolic and signaling programs in a wide-variety of cell types. The Cytometry & Cell Sorting Core (CCSC) has established itself as a vital and growing component of the Columbia University Diabetes Research Center (DRC), as it addressed the research needs of a growing number of DRC investigators who study immunological, developmental, and metabolic aspects of diabetes. Created in 2010 in partnership with the Columbia Center for Translational Immunology (CCTI) and the Department of Medicine (DOM) with a substantial University investment as well as NIH support, in the past 5 years CCSC has assisted 22 DRC users currently funded by 55 NIH grants, including 30 NIDDK grants. The data generated with the CCSC contributed to 63 publications and 17 new grants. The implementation of CCSC has been essential to support many aspects of diabetes research at Columbia. Since its establishment, the demand for CCSC services has steadily increased, from 60 hours per week with two analyzers to over 130 hours per week with three analyzers, and from 25 hours per week with one sorter to over 60 hours per week with two sorters. We expect usage to continue to grow as new recruits join the DRC, and established investigators take advantage of resources and technologies offered by the Core. Overall, CCSC will assist investigators with the following two aims: Aim 1 – To quantify and phenotypically characterize cell populations that contribute to the metabolic, immunologic and developmental programs of diabetes and its complications. CCSC leverages advanced technologies in fluorescent imaging at the single cell resolution to support the analysis of many different cell types in both human and animal tissues, as well as humanized mouse models of Type 1 diabetes. Aim 2 – To purify populations of cells of relevance to diabetes and its complications. Using fluorescence-activated cell sorting, CCSC supports DRC investigators to purify individual populations of cells for in vitro culture, in vivo implantation or molecular characterization including genetic and transcriptional profiling, protein purification and signaling studies, and for functional analysis including T cell activation, proliferation and migration studies. To achieve these aims, the CCSC has established standard operating procedures to: (i) Assure quality control and reproducibility; (ii) Prioritize investigator use; (iii) Monitor Core use; and (iv) Adapt to new technologies and to the needs of the Columbia Research Base.
流式细胞术是对细胞进行定量和表型分型的重要工具,所述细胞定义与以下相关的途径: 糖尿病以前仅限于分析循环免疫细胞,从组织中提取细胞的进展 使流式细胞术的能力扩展到发育,代谢和信号的研究 在各种各样的细胞类型的程序。细胞计数和细胞分选核心(CCSC)已成为 哥伦比亚大学糖尿病研究中心(DRC)的重要和不断增长的组成部分,因为它解决了 越来越多的研究免疫学、发育学和免疫学的DRC研究人员的研究需求, 糖尿病的代谢方面。2010年与哥伦比亚翻译中心合作创建 免疫学(CCTI)和医学系(DOM)与大量的大学投资,以及 在NIH的支持下,在过去的5年里,CCSC已经帮助了22个DRC用户,目前由55个NIH赠款资助, 包括30个NIDDK赠款。CCSC生成的数据为63篇出版物和17篇 新的赠款。CCSC的实施对于支持糖尿病研究的许多方面至关重要, 哥伦比亚。自成立以来,对CCSC服务的需求稳步增长,从每小时60小时 从两台分析仪每周工作超过130小时,到三台分析仪每周工作25小时, 从一个分拣员到两个分拣员每周60多个小时。我们预计随着新员工的加入, 加入刚果民主共和国,并建立调查人员利用资源和技术提供的核心。 总体而言,CCSC将协助研究者实现以下两个目标:目标1 -量化和表型 表征有助于代谢、免疫和发育的细胞群 糖尿病及其并发症。CCSC利用先进的荧光技术 以单细胞分辨率成像,以支持对人类和哺乳动物中许多不同细胞类型的分析。 动物组织以及1型糖尿病的人源化小鼠模型。目的2 -纯化 与糖尿病及其并发症相关的细胞。使用荧光激活细胞分选,CCSC 支持DRC研究人员纯化单个细胞群,用于体外培养、体内植入或 分子表征,包括遗传和转录谱分析、蛋白质纯化和信号传导 研究和功能分析,包括T细胞活化、增殖和迁移研究。实现 为了实现这些目标,CCSC制定了标准操作程序,以:(i)确保质量控制, 重现性;(ii)优先考虑研究者使用;(iii)监测核心药物的使用;以及(iv)适应新技术, 哥伦比亚研究基地的需求

项目成果

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Remi J Creusot其他文献

Remi J Creusot的其他文献

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{{ truncateString('Remi J Creusot', 18)}}的其他基金

Unraveling the tolerogenic potential of lymph node fibroblastic reticular networks in autoimmune diabetes
揭示自身免疫性糖尿病中淋巴结成纤维细胞网状网​​络的耐受潜力
  • 批准号:
    10672991
  • 财政年份:
    2022
  • 资助金额:
    $ 14.9万
  • 项目类别:
Five-laser Aurora spectral flow cytometer
五激光 Aurora 光谱流式细胞仪
  • 批准号:
    10426837
  • 财政年份:
    2022
  • 资助金额:
    $ 14.9万
  • 项目类别:
Unraveling the tolerogenic potential of lymph node fibroblastic reticular networks in autoimmune diabetes
揭示自身免疫性糖尿病中淋巴结成纤维细胞网状网​​络的耐受潜力
  • 批准号:
    10624501
  • 财政年份:
    2022
  • 资助金额:
    $ 14.9万
  • 项目类别:
In vivo development and reactivity of human autoreactive T cells
人类自身反应性 T 细胞的体内发育和反应性
  • 批准号:
    10290314
  • 财政年份:
    2018
  • 资助金额:
    $ 14.9万
  • 项目类别:
In vivo development and reactivity of human autoreactive T cells
人类自身反应性 T 细胞的体内发育和反应性
  • 批准号:
    10519104
  • 财政年份:
    2018
  • 资助金额:
    $ 14.9万
  • 项目类别:
In vivo development and reactivity of human autoreactive T cells
人类自身反应性 T 细胞的体内发育和反应性
  • 批准号:
    10054162
  • 财政年份:
    2018
  • 资助金额:
    $ 14.9万
  • 项目类别:
Engineering and targeting novel antigen-specific tolerogenic interfaces
工程设计和靶向新型抗原特异性耐受性界面
  • 批准号:
    8969963
  • 财政年份:
    2015
  • 资助金额:
    $ 14.9万
  • 项目类别:
Engineering and targeting novel antigen-specific tolerogenic interfaces
工程设计和靶向新型抗原特异性耐受性界面
  • 批准号:
    9118059
  • 财政年份:
    2015
  • 资助金额:
    $ 14.9万
  • 项目类别:
Cytometry Core
细胞计数核心
  • 批准号:
    10588840
  • 财政年份:
    2002
  • 资助金额:
    $ 14.9万
  • 项目类别:
Flow Cytometry Shared Resource
流式细胞术共享资源
  • 批准号:
    10661659
  • 财政年份:
    1997
  • 资助金额:
    $ 14.9万
  • 项目类别:

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