Flow Cytometry Shared Resource

流式细胞术共享资源

• 批准号: 10661659
• 负责人: Remi J Creusot
• 金额: $ 12.54万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-04 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661659
• 关键词: Cancer Biology; Cancer Center Support Grant; Cell Separation; Cell surface; Cells; Color; Complex; Computer software; Computers and Advanced Instrumentation; Conceptions; Consultations; Data; Data Analyses; Dedications; Development; Doctor of Philosophy; Education; Equipment; Event; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Goals; Hematopoietic; Herbert Irving Comprehensive Cancer Center; Hour; Human Resources; Immune; Immunology; Infiltration; Infrastructure; Intracellular Signaling Proteins; Journals; Lasers; Leadership; Lymphoid Cell; Maintenance; Malignant Neoplasms; Manuscripts; Metabolic Marker; Myeloid Cells; Nature; Needs Assessment; Peer Review; Population; Preparation; Protocols documentation; Publications; Qualifying; Reproducibility; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Scientist; Secure; Services; Solid; Sorting; Speed; Stains; Techniques; Technology; Time; Training; Training and Education; Trees; United States National Institutes of Health; Walking; anticancer research; cancer cell; cancer immunotherapy; cancer therapy; complex data; cost effective; cost effectiveness; data acquisition; density; design; detector; empowerment; experience; high dimensionality; high standard; high throughput screening; indexing; insight; instrument; instrumentation; leukemia/lymphoma; member; multidimensional data; neoplastic cell; new technology; novel; programs; prospective; service providers; stem cells; success; technology development; technology validation; tool; transcription factor; translational immunology; tumor; tumor immunology

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY The Flow Cytometry Shared Resource (FCSR) gives access to flow cytometry analysis and cell sorting instruments and services to Herbert Irving Comprehensive Cancer Center (HICCC) members. Under the leadership of Remi Creusot, PhD, the FCSR provides: (1) multi-color panel flow data acquisition and analysis using flow cytometry analyzers, including high throughput screening (HTS), (2) flow cytometry cell sorting (four- way or six-way or plate) using high speed cell sorters by dedicated operators of FCSR, including single cell index sorting, and (3) comprehensive consultation, panel design, hands-on training, troubleshooting, and data analysis services by PhD-level FCSR scientists. This broad and inclusive package of services enables HICCC investigators to analyze tumor cell populations and their microenvironment, including the tumor-infiltrating lymphoid and myeloid cells at the single cell level. During the current project period (2014-2019), the FCSR has been integrated with the flow cytometry core of the Columbia Center for Translational Immunology (CCTI). The merging of these facilities has secured access to a broader range of state-of-the-art instrumentation and brought advanced expertise in immunology to the research of HICCC flow cytometry users. HICCC members now have access to nine instruments, including five analyzers and four sorters. In addition, two of the existing instruments/analyzers have been upgraded to 18-20 detectors. Moreover, a new Influx sorter has been acquired to expand the capacity of sorting services. Upgrades in consultation services have further empowered the research of HICCC members who now benefit from face-to-face consultation on panel design, protocol optimization, and hands-on training on data acquisition, analysis, and experimental troubleshooting essential to maximize the impact of new advanced instruments for high dimensional multi-color panels. Based on its advanced instrumentation, highly qualified staff, broad portfolio of specialized services, easy accessibility, and cost-effective services, the FCSR is the flow cytometry service provider of choice for HICCC members. The FCSR will continue to incorporate the latest technologies and to provide high quality cell sorting, analytical and consultation services to HICCC members to empower their understanding of cancer biology and to enable new breakthroughs in cancer therapy. Over the current project period, the capabilities of the FCSR have been utilized by 81 HICCC members, provided key data and insights to support the success of more than 46 HICCC member peer-reviewed publications, including 23 contributions in journals with impact factor >10, of which 12 were in journals with an impact factor >20 (Nature, Cell Stem Cell, Cancer Cell, Cancer Discovery), and currently support research for 21 NIH-funded research grants (11 from NCI).

流式细胞术共享资源：项目总结 流式细胞仪共享资源（FCSR）提供流式细胞仪分析和细胞分选的访问 仪器和服务的赫伯特欧文综合癌症中心（CDC）的成员。下 在Remi Creusot博士的领导下，FCSR提供：（1）多色面板流量数据采集和分析 使用流式细胞术分析仪，包括高通量筛选（HTS），（2）流式细胞术细胞分选（四- 方法或六路或板）使用高速细胞分选仪由专用操作员FCSR，包括单细胞索引 分类，以及（3）全面咨询，面板设计，实践培训，故障排除和数据分析 由博士级FCSR科学家提供服务。这一广泛而包容的服务包使CIMCC能够 研究人员分析肿瘤细胞群及其微环境，包括肿瘤浸润性细胞， 淋巴样和髓样细胞在单细胞水平。在本项目期间（2014-2019年）， 已与哥伦比亚转化免疫学中心（CCTI）的流式细胞术核心整合。的 这些设施的合并确保了获得更广泛的最先进的仪器， 在免疫学方面的先进专业知识，以研究的CDICC流式细胞仪用户。目前，CICC成员 可使用九台仪器，包括五台分析仪和四台分拣机。此外，现有的两个 仪器/分析仪已升级到18-20个探测器。此外，还购置了一台新的Influx分选机 扩大分拣服务能力。咨询服务的升级进一步增强了 研究的CNOCC成员谁现在受益于面对面的咨询小组的设计，协议 优化，并在数据采集，分析和实验故障排除方面进行实践培训， 最大限度地发挥新的先进仪器对高维多色面板的影响。基于其 先进的仪器设备、高素质的员工、广泛的专业服务组合、易于访问，以及 FCSR提供高性价比的服务，是CSCCC会员首选的流式细胞仪服务提供商。的 FCSR将继续采用最新技术，提供高质量的细胞分选、分析和 咨询服务，以增强他们对癌症生物学的理解，并使新的 癌症治疗的重大突破在本项目期间，已利用了FCSR的能力 由81个亚太经合组织成员组成，提供了关键数据和见解，以支持超过46个亚太经合组织成员的成功 同行评审的出版物，包括影响因子>10的期刊上的23篇文章，其中12篇在 影响因子>20的期刊（Nature，Cell Stem Cell，Cancer Cell，Cancer Discovery），目前支持 研究21 NIH资助的研究赠款（11从NCI）。