Super-multiplex vibrational imaging in living cells
Super-multiplex vibrational imaging in living cells
批准号:
9921414
负责人:
Wei Min
金额:
$31.18万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-05-01 至 2022-04-30
关键词:
AdoptedAlkynesAmplifiersApoptosisAzidesBiological ProcessBiotinCell NucleusCell membraneCellsCellular biologyChemicalsClinicalColorComplexCrystallizationCytokinesisDetectionDevelopmentDiseaseDyesEndoplasmic ReticulumEngineeringEventFluorescenceGenerationsGeneticGoalsGolgi ApparatusImageImaging TechniquesImmunologyLabelLasersLightLinkLipidsLysosomesMalignant NeoplasmsMethodsMicroscopeMicroscopyMitochondriaMolecularMolecular TargetNatureNervous system structureNeurobiologyOpticsOrganellesPhysiologic pulseProcessProteinsPublishingPumpRegulationResearch PersonnelRoleS-nitro-N-acetylpenicillamineSeriesShapesSpecificitySpeedStructural ProteinStructure-Activity RelationshipSystemSystems BiologyTechniquesTechnologyTestingTimeTumor Biologybiological systemsbiomaterial compatibilitycomplex biological systemsdesigndetectorhybrid proteinimaging capabilitiesimaging geneticsimaging modalityimaging platformimaging probeimprovedinstrumentationinterestmacromoleculemultiplexed imagingnanomolarnext generationnovelnovel therapeuticsoptical imagingsingle moleculetumor heterogeneityvibration
中文摘要
总结
生物系统的组织和功能本质上是复杂且相互关联的
通过一系列涉及多个交互组件的分层网络。因此,
同时可视化活细胞内大量不同的分子种类
对于全面理解这些生物过程来说变得不可或缺。正如我们
进入系统生物学时代,这种超多重成像能力将具有变革性
跨越各个领域,包括揭示神经系统的结构与功能关系;
了解肿瘤异质性;研究细胞过程中的大分子编排
调节,以及揭示活细胞各种细胞器之间复杂的相互作用。
该项目的目标是开发通用的超多重光学显微镜平台
用于同时对大量(超过 20 个)内部特定分子目标进行成像
活细胞,这是传统方法的一个重要但难以实现的目标,例如
荧光。为此,我们建议将新兴的电子预谐振激励耦合起来
拉曼散射 (epr-SRS) 显微镜,提供纳摩尔级检测灵敏度和窄
化学特异性,具有由三键共轭光组成的新型振动探针
吸收染料。第一代技术最近已发表,展示了
生物系统中 24 色成像的记录(L. Wei … W. Min. Nature, 544, 465, 2017)。
面向下一代技术,我们制定了系统的计划
如何将这一概念具体化为一个更强大的平台,以实现高速、高
灵敏度,活细胞中特定蛋白质和细胞器的超多重振动成像。
我们建议建造新的显微镜仪器以显着提高成像效果
速度提高几个数量级(具体目标 1),并设计新型 epr-SRS 振动探头
具有扩展的调色板、卓越的检测灵敏度、细胞器靶向特异性和
对特定蛋白质的遗传可编码性(特定目标 2)。伴随着这些技术
进展,然后我们将应用它来探测多个细胞器内的系统级相互作用
胞质分裂和细胞凋亡动态过程中的蛋白质(具体目标 3)。
如果成功实施,我们将建立一个变革性的成像平台
允许研究人员研究活细胞中前所未有的大量生物分子
具有卓越的灵敏度、靶向特异性、标记多功能性和生物相容性。的
由此产生的超多重光学显微镜将在解开复杂的问题中找到广泛的应用
生物系统,例如细胞生物学、神经生物学、免疫学和肿瘤生物学。
英文摘要
Summary
Biological systems are inherently complex and interrelated, as they organize and function
through a series of hierarchical networks involving multiple interacting components. Hence,
simultaneously visualizing a large number of distinct molecular species inside living cells has
become indispensable for understanding these biological processes in a holistic manner. As we
enter the era of systems biology, such super-multiplex imaging capability will be transformative
across various fields including revealing structure–function relationships in nervous systems;
understanding tumor heterogeneity; studying macromolecules choreography during cell
regulation, as well as revealing intricate interactions among various organelles of living cells.
The goal of this project is to develop a general super-multiplex optical microscopy platform
for simultaneously imaging a large number (more than 20) of specific molecular targets inside
live cells, an important but otherwise intractable goal by conventional methods such as
fluorescence. To do so, we propose to couple the emerging electronic pre-resonance stimulated
Raman scattering (epr-SRS) microscopy, offering nanomolar detection sensitivity and narrow
chemical specificity, with novel vibrational probes consisting of triple-bond-conjugated light-
absorbing dyes. The first-generation technique has been recently published, demonstrating a
record of 24-color imaging in biological systems (L. Wei … W. Min. Nature, 544, 465, 2017).
Moving towards the next-generation technology, we have laid out systematic plans as to
how to crystallize this concept into a much more powerful platform to achieve high-speed, high-
sensitivity, super-multiplex vibrational imaging of specific proteins and organelles in living cells.
We propose to construct new microscope instrumentations to significantly boost the imaging
speed by orders of magnitude (Specific Aim 1), and engineer novel epr-SRS vibrational probes
with expanded color palette, superior detection sensitivity, organelle targeting specificity and
genetic encodability to specific proteins (Specific Aim 2). Accompanied by these technical
developments, we will then apply it to probe systems-level interactions within multiple organelles
and proteins during dynamical processes of cytokinesis and apoptosis (Specific Aim 3).
If successfully implemented, we will establish a transformative imaging platform that could
allow researchers to interrogate an unprecedented large number of bio-molecules in living cells
with superb sensitivity, targeting specificity, labeling versatility, and biocompatibility. The
resulting super-multiplex optical microscopy would find wide applications in unraveling complex
biological systems such as cell biology, neurobiology, immunology, and tumor biology.
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会议论文
Super-multiplex optical imaging: development of novel spectroscopy and probes to illuminate complex biomedicine
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依托单位:
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资助金额:$37.06万
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财政年份:2020
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High-resolution volumetric imaging of metabolic activity in tissues and its application to tumor metabolism
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批准号:10117249
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资助金额:$38.87万
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财政年份:2020
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负责人:Wei Min
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依托单位:
Ultrahigh-resolution and single-molecule stimulated Raman scattering (SRS) microscopy
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批准号:9899269
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项目类别:
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资助金额:$31.54万
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财政年份:2019
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负责人:Wei Min
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依托单位:
Ultrahigh-resolution and single-molecule stimulated Raman scattering (SRS) microscopy
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批准号:10377375
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项目类别:
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资助金额:$31.78万
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财政年份:2019
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负责人:Wei Min
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依托单位:
Super-multiplex vibrational imaging in living cells
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批准号:10163876
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项目类别:
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资助金额:$31.23万
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财政年份:2018
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负责人:Wei Min
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依托单位:
Optical imaging of small bio-molecules in living cells and tissues by nonlinear Raman microscopy coupled with vibrational tags
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批准号:9298651
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项目类别:
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资助金额:$32.14万
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财政年份:2015
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负责人:Wei Min
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依托单位:
Stimulated emission reduced fluorescence (SERF) for breaking and extending the fundamental imaging-depth of two photon microscopy
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批准号:9025791
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项目类别:
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资助金额:$20.0万
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财政年份:2015
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负责人:Wei Min
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依托单位:
Ultra-deep tissue imaging by super-nonlinear fluorescence microscopy
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批准号:8857201
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项目类别:
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资助金额:$20.0万
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财政年份:2014
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负责人:Wei Min
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依托单位:
Ultra-deep tissue imaging by super-nonlinear fluorescence microscopy
-
批准号:8769558
-
项目类别:
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资助金额:$24.0万
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财政年份:2014
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负责人:Wei Min
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依托单位:
Label-Free Chemical Imaging for Biological Applications
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批准号:8352315
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项目类别:
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资助金额:$240.0万
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财政年份:2012
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负责人:Wei Min
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依托单位:
海外基金