Adipocyte Biology and Molecular Nutrition (ABMN)

脂肪细胞生物学和分子营养（ABMN）

• 批准号: 9926270
• 负责人: Nada A. Abumrad
• 金额: $ 15.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9926270
• 关键词: 2-acylglycerol O-acyltransferase; Abbreviations; Acid Lipase; Adenoviruses; Adipocytes; Adipose tissue; Animal Model; Autophagocytosis; BCL2 gene; Basic Science; CCL2 gene; CCL3 gene; CD11b Antigens; CD11c Antigens; COL6A1; COL6A3; CXCL14 gene; Cardiovascular Diseases; Cell Line; Cell Respiration; Cell Size; Cells; Cellular Assay; Cellular biology; Clinical; Clinical Investigator; Clinical Research; Clinical Sciences; Collagen Type IV; Collagen Type VI; Comb animal structure; Complement; Consultations; Core Facility; Cytolysis; Data; Deposition; Diabetes Mellitus; Digestive System Disorders; Diglycerides; Disease; EGF gene; Eating; Endoplasmic Reticulum; Energy Metabolism; Ensure; Equipment; Estrogen receptor positive; Evaluation; Extracellular Matrix; FRAP1 gene; Fatty Acids; Fibrinogen; Fibrosis; Fluorescence-Activated Cell Sorting; Fostering; Funding; Gene Expression; Glucose; Health; Homeostasis; Hormones; Human; ITGAM gene; ITGAX gene; Image; Immunoassay; Individual; Infiltration; Inflammation; Institution; Integral Membrane Protein; Leadership; Ligands; Lipids; Lipolysis; Liver; MFAP1 gene; Malignant Neoplasms; Metabolic; Metabolic dysfunction; Metabolism; Microtubule-Associated Proteins; Mitochondria; Mitochondrial DNA; Mixed Function Oxygenases; Molecular; Morphology; Mucins; Mus; Muscle; Myoblasts; Nutritional; Obesity; Organ; Organelles; Oxidative Phosphorylation; PLA2G6 gene; Pathology; Pathway interactions; Phenotype; Phosphotransferases; Physiology; Procedures; Proteins; Protocols documentation; RNA; Reactive Oxygen Species; Regulation; Research; Research Activity; Research Personnel; Role; Services; Skeletal Muscle; Structure; System; TNF gene; Tissues; Titrations; Training; Universities; Washington; Work; adipocyte biology; adipokines; chemokine; comorbidity; cost; cost effective; experience; hands on instruction; in vivo; inhibitor/antagonist; insulin sensitivity; macrophage; mannose receptor; medical schools; metabolic abnormality assessment; nutrition; programs; protein expression; pyruvate carrier; receptor; sex; trafficking; transcription factor

PROJECT SUMMARY/ABSTRACT – Adipocyte Biology and Molecular Nutrition Core Coordination of substrate use between key metabolic tissues such as adipose, muscle and liver is essential for energy homeostasis, insulin sensitivity and overall health. Adipose tissue is central to energy regulation with roles in fuel storage and mobilization, and the secretion of factors (i.e. adipokines) that influence food intake, energy expenditure, and the functioning of key organs such as the vasculature, muscle and liver. Obesity- induced expansion of adipose tissue often associates with metabolic dysfunction, systemic inflammation, and comorbid diseases such as cardiovascular disease, diabetes and cancer. The purpose of the Adipocyte Biology and Molecular Nutrition (ABMN) Core, established in 2006, is to foster and facilitate research related to pathways influencing adipose tissue structure/function and the function of other key metabolic tissues such as muscle and liver. The Core provides researchers with cost-effective access to specialized equipment, expert training and reliable services that are not easily available elsewhere at Washington University. The centralized provision of targeted and specialized high quality services by the ABMN Core increases efficiency of research efforts and reduces barriers for clinical and young investigators who wish to conduct sophisticated nutrition- related research but do not have the expertise or facilities/equipment within their own programs. The ABMN Core is interactive and tries whenever possible to tailor services to meet the needs of NORC investigators. The ABMN Core provides the following services: 1) Cell lines (mouse and human pre-adipocytes and myoblasts) and culture protocols; 2) Adipose tissue phenotyping (cell sizing, macrophage infiltration/polarization, and fibrosis); 3) Optimized tissue lysis, RNA isolation, gene and protein expression for nutrition/obesity related targets; 40 Mitochondrial physiology of tissues or isolated mitochondria; 5) Cell biology and organelle imaging to visualize substrate processing e.g. lipid deposition/trafficking; 6) Access to specialized equipment; and 7) Consultation and training provided by the Core leadership and staff with strong synergistic expertise in molecular research in nutrition and obesity.

项目摘要/摘要-脂肪细胞生物学和分子营养核心 在脂肪、肌肉和肝脏等关键代谢组织之间协调底物使用是必不可少的 能量平衡、胰岛素敏感性和整体健康。脂肪组织是能量调节的中心， 在燃料储存和动员以及影响食物摄入量的因素(即脂肪因子)的分泌方面的作用， 能量消耗，以及血管、肌肉和肝脏等关键器官的功能。肥胖- 脂肪组织的诱导扩张通常与代谢障碍、全身性炎症和 心血管疾病、糖尿病和癌症等并存疾病。脂肪细胞的用途 生物学和分子营养学(ABMN)成立于2006年，其核心是促进和促进与 影响脂肪组织结构/功能和其他关键代谢组织功能的途径，如 肌肉和肝脏。核心为研究人员提供了经济高效的专业设备访问途径，专家 培训和可靠的服务，在华盛顿大学的其他地方是不容易获得的。中央集权 ABMN核心提供有针对性和专业化的高质量服务提高了研究效率 为希望进行复杂营养研究的临床和年轻研究人员做出努力并减少障碍- 相关研究，但在自己的项目中没有专业知识或设施/设备。ABMN CORE是交互式的，并尽可能地尝试定制服务以满足NORC调查人员的需求。这个 ABMN Core提供以下服务：1)细胞系(小鼠和人的前脂肪细胞和成肌细胞) 和培养方案；2)脂肪组织表型(细胞大小，巨噬细胞渗透/极化，以及 纤维化)；3)优化组织裂解、RNA分离、营养/肥胖相关基因和蛋白质表达 靶点；40组织或分离线粒体的线粒体生理学；5)细胞生物学和细胞器成像 使底物加工可视化，例如脂质沉积/贩运；6)获得专门设备；和7) 由核心领导层和具有很强协同能力的员工提供咨询和培训 营养与肥胖的分子研究。