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Evaluation of Cartilage Tissue Engineering Strategies by IR Imaging

通过红外成像评估软骨组织工程策略

基本信息

批准号：
9926711
负责人：
Nancy Pleshko
金额：
$ 30.5万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9926711
关键词：
Address Animal Model Area Biochemical Biological Models Biomechanics Biopsy Cartilage Cell Count Cells Chondrocytes Clinical Clinical assessments Collagen Data Defect Development Engineering Evaluation Exhibits Family suidae Fiber Optics Functional disorder Funding Growth Factor Harvest Histologic Human Hyaluronic Acid Image Implant In Vitro Individual Least-Squares Analysis Magnetic Resonance Imaging Mechanics Methods Miniature Swine Modality Modeling Modulus Molecular Molecular Profiling Molecular Structure Monitor Musculoskeletal Near-Infrared Spectroscopy Operative Surgical Procedures Optical Methods Outcome Play Production Property Proteoglycan Protocols documentation Regenerative Medicine Role Series Spectroscopy, Fourier Transform Infrared Techniques Technology Testing Therapeutic Thick Time Tissue Engineering Tissues Translating Translations Water Work X-Ray Computed Tomography base cartilage repair cartilaginous cell type clinical development clinical predictors clinically relevant design implantation in vivo laboratory experiment mechanical properties microCT mimetics minimally invasive novel preimplantation repaired scaffold subchondral bone success technique development tissue regeneration tissue repair

项目摘要

Summary: In spite of progress in understanding the pathophysiology of OA, there remain no definitive non-surgical treatment options. Further, tissue engineering strategies designed to produce cartilage-mimetic materials have seen only limited success; a significant impediment is the inability to fully assess the molecular structure of developing tissue such that optimal constructs for implantation can be identified. Recently, we demonstrated that the technique of non-destructive near infrared spectroscopy (NIRS) analysis can be utilized to assess the primary molecular components of native and engineered cartilage. NIRS data correlates strongly to water and matrix content (collagen and proteoglycan) in these tissues, and to mechanical properties. Having validated the NIR technology for cartilage assessment, we propose to extend our studies to address an important emerging area of musculoskeletal tissue engineering, identification of individual constructs suitable for clinical implantation. These studies are based on the principle that increases in construct maturation prior to implantation can be utilized to predict in vivo success. We hypothesize that maturation of the cartilaginous matrix can be monitored by NIRS, and that the spectral data can be used as a predictor of clinical success. We will address this in a series of in vitro and in vivo studies that evaluate construct development using NIRS analysis. A cell-seeded hyaluronic acid (HA) scaffold, previously shown to be effective at promoting matrix production and the development of functional material properties, will serve as the model system for implantation into a porcine model of cartilage repair. The proposed NIRS technology would establish a novel, non-destructive modality for pre-implantation evaluation of developing engineered tissues for clinical implantation. There are currently no minimally-invasive methods available for the clinical assessment of cartilage repair tissue maturation based on molecular signatures. The development of clinically-relevant optical methods to evaluate full-depth composition of tissues in a large animal model will provide a strong underpinning for translation of this technique into the clinical setting. Such analyses would also represent a fundamentally new and powerful approach for evaluating and guiding cartilage therapeutics. The possibility of one non-destructive modality that can be used in vitro during laboratory experiments of developing tissues to assess composition and identify constructs for implantation, as well as after the constructs are implanted clinically, would indeed be a powerful technique that could play a central role in the regenerative medicine field.

概括：尽管对 OA 病理生理学的了解取得了进展，但仍然没有明确的非手术治疗方法治疗方案。此外，旨在生产软骨模拟材料的组织工程策略已经只取得了有限的成功；一个重大障碍是无法充分评估分子结构发育组织，以便可以确定植入的最佳结构。最近，我们展示了无损近红外光谱（NIRS）分析技术可用于评估天然和工程软骨的主要分子成分。 NIRS 数据与水和这些组织中的基质含量（胶原蛋白和蛋白多糖）以及机械性能。经验证后用于软骨评估的近红外技术，我们建议扩展我们的研究以解决一个重要的新兴问题肌肉骨骼组织工程领域，识别适合临床的个体结构植入。这些研究基于这样的原理：在构建体成熟之前增加植入可用于预测体内成功。我们假设软骨的成熟矩阵可以通过 NIRS 进行监测，并且光谱数据可以用作临床成功的预测因子。我们将在一系列使用 NIRS 评估构建体开发的体外和体内研究中解决这个问题分析。细胞接种的透明质酸 (HA) 支架，先前已被证明可有效促进基质功能材料特性的生产和开发，将作为模型系统植入猪软骨修复模型。拟议的 NIRS 技术将为植入前建立一种新颖的非破坏性模式评估开发用于临床植入的工程组织。目前尚无微创基于分子水平可用于临床评估软骨修复组织成熟度的方法签名。开发临床相关光学方法来评估组织的全面深度组成在大型动物模型中的研究将为该技术转化为临床提供强有力的基础环境。此类分析也将代表一种全新且强大的评估和评估方法。指导软骨治疗。一种可在体外使用的非破坏性方式的可能性发育组织的实验室实验，以评估成分并确定植入结构，例如在将结构植入临床后，这确实是一种强大的技术，可以发挥在再生医学领域发挥核心作用。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Non-destructive NIR spectral imaging assessment of bone water: Comparison to MRI measurements.

骨水的非破坏性NIR光谱成像评估：与MRI测量值进行比较。

DOI：
10.1016/j.bone.2017.06.015
发表时间：
2017-10
期刊：
Bone
影响因子：
4.1
作者：
Rajapakse CS;Padalkar MV;Yang HJ;Ispiryan M;Pleshko N
通讯作者：
Pleshko N

Vibrational spectroscopy and imaging: applications for tissue engineering.

DOI：
10.1039/c7an01055a
发表时间：
2017-10-23
期刊：
The Analyst
影响因子：
0
作者：
Querido W;Falcon JM;Kandel S;Pleshko N
通讯作者：
Pleshko N

Unusual fragmentation pathways in collagen glycopeptides.

DOI：
10.1007/s13361-013-0624-y
发表时间：
2013-07
期刊：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
影响因子：
3.2
作者：
Perdivara, Irina;Perera, Lalith;Sricholpech, Marnisa;Terajima, Masahiko;Pleshko, Nancy;Yamauchi, Mitsuo;Tomer, Kenneth B.
通讯作者：
Tomer, Kenneth B.

Nondestructive assessment of tissue engineered cartilage based on biochemical markers in cell culture media: application of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy.