Infrared fiber optic imaging of degenerative cartilage

退行性软骨的红外光纤成像

• 批准号: 7934494
• 负责人: Nancy Pleshko
• 金额: $ 36.92万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7934494
• 关键词: Biopsy; Cartilage; Characteristics; Classification; Clinical; Collagen; Collagen Type II; Complex; Data; Decision Making; Defect; Degenerative polyarthritis; Detection; Development; Diagnostic; Disease; Evaluation; Exhibits; Fiber Optics; Foundations; Fourier Transform; Gene Expression; Goals; Healed; Histologic; Histopathologic Grade; Human; Image; Image Analysis; In Situ; Intervention; Investigation; Knowledge; Laboratories; Link; Longitudinal Studies; Methodology; Methods; Modeling; Modification; Molecular; Molecular Structure; Monitor; Musculoskeletal Diseases; Operative Surgical Procedures; Oryctolagus cuniculus; Outcome; Patients; Pattern Recognition; Play; Pre-Clinical Model; Procedures; Process; Production; Proteoglycan; Role; Staging; Surface; System; Techniques; Technology; Therapeutic; Therapeutic Intervention; Time; Tissues; Validation; Work; Wound Healing; articular cartilage; base; cartilage repair; clinical application; computerized data processing; crosslink; data acquisition; healing; improved; in vivo; infrared spectroscopy; mid infrared spectroscopy; optical imaging; osteochondral tissue; primary outcome; public health relevance; regenerative; repaired; response; technique development; therapy design; therapy development; tissue regeneration; tool

DESCRIPTION (provided by applicant): Osteoarthritis is a highly prevalent, progressively disabling musculoskeletal disease characterized by degeneration of articular cartilage. A significant barrier to the development of therapy for this disorder is the difficulty in assessing therapeutic interventions designed to induce the production of cartilage repair tissue. We propose the use of Fourier transform infrared (FTIR) spectroscopic analysis towards this goal. To date, our laboratory has demonstrated a significant correlation between spectra obtained from the cartilage surface using a mid-infrared fiber optic probe (mid-IFOP), and histological signs of early cartilage degradation which occur prior to macroscopic damage. We propose to build on this by extending our methodology to the setting of cartilage repair, where we envision use of the mid-IFOP as an arthroscopic diagnostic tool to evaluate repair tissue. This will be accomplished by optimization of spectral acquisition and application of chemometric methods (statistically based pattern-recognition methods used to identify or classify materials based on their spectral characteristics) to correlate spectral data with molecular characteristics of the tissue. This will provide data which is clinically useful in assessing cartilage repair while adding minimal time to arthroscopic procedures. Our methodology will first be developed in a longitudinal study of the repair process in the rabbit, and then applied to classification of repair tissue obtained by biopsy from patients. A key point in the latter analysis will be to establish the correlation between infrared spectroscopy-derived repair tissue classification and clinical outcomes. These data will lay the foundation for subsequent in vivo investigation of ongoing cartilage repair in humans. In summary, we note that there are currently no non-destructive methods available for detection and classification of subtle molecular changes in cartilage repair tissue, and for relating such changes to histologic grade and clinical outcome; therefore, the analyses we propose may represent an important advance in the emergent field of OA therapeutics. PUBLIC HEALTH RELEVANCE Articular cartilage exhibits limited capacity for self-repair, and chondral defects typically progress to widespread degradation without intervention. The development of the mid infrared fiber optic probe application could offer the ability to monitor cartilage repair tissue molecular characteristics in situ, and thus permit appropriate interventions to be undertaken on an ongoing basis. Therefore, the development of this technique may play a truly central role in the exceedingly important field of tissue regeneration by offering this capability.

描述（由申请人提供）：骨关节炎是一种高度流行的、进行性致残的肌肉骨骼疾病，其特征是关节软骨退化。开发这种疾病的治疗的一个重要障碍是难以评估旨在诱导软骨修复组织产生的治疗干预。我们建议使用傅里叶变换红外（FTIR）光谱分析实现这一目标。迄今为止，我们的实验室已经证明了使用中红外光纤探针（中IFOP）从软骨表面获得的光谱与宏观损伤之前发生的早期软骨降解的组织学迹象之间的显著相关性。我们建议通过将我们的方法扩展到软骨修复的设置来建立这一点，我们设想使用中期IFOP作为关节镜诊断工具来评估修复组织。这将通过优化光谱采集和应用化学计量学方法（基于统计的模式识别方法，用于根据材料的光谱特性对其进行识别或分类）来实现，以将光谱数据与组织的分子特性相关联。这将提供临床上用于评估软骨修复的数据，同时增加关节镜手术的最短时间。我们的方法将首先在兔子修复过程的纵向研究中开发，然后应用于通过患者活检获得的修复组织的分类。后一种分析的关键点将是建立红外光谱衍生的修复组织分类和临床结果之间的相关性。这些数据将为后续人体软骨修复的体内研究奠定基础。总之，我们注意到，目前还没有非破坏性的方法可用于检测和分类软骨修复组织中的细微分子变化，并将这些变化与组织学分级和临床结果相关联;因此，我们提出的分析可能代表了OA治疗领域的重要进展。 公共卫生相关性关节软骨自我修复的能力有限，软骨缺损通常会在不进行干预的情况下发展为广泛的退化。中红外光纤探针应用的发展可以提供原位监测软骨修复组织分子特征的能力，从而允许在持续的基础上进行适当的干预。因此，该技术的发展可能通过提供这种能力在极其重要的组织再生领域中发挥真正的核心作用。

项目成果

Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment.

• DOI: 10.1016/j.joca.2010.01.006
• 发表时间: 2010-05
• 期刊: OSTEOARTHRITIS AND CARTILAGE
• 影响因子: 7
• 作者: Gurkan, I.;Ranganathan, A.;Yang, X.;Horton, W. E., Jr.;Todman, M.;Huckle, J.;Pleshko, N.;Spencer, R. G.
• 通讯作者: Spencer, R. G.

Differences in infrared spectroscopic data of connective tissues in transflectance and transmittance modes.

• DOI: 10.1016/j.aca.2013.03.053
• 发表时间: 2013-05-24
• 期刊: Analytica chimica acta
• 影响因子: 6.2
• 作者: Hanifi A;McGoverin C;Ou YT;Safadi F;Spencer RG;Pleshko N
• 通讯作者: Pleshko N

Near infrared spectroscopic evaluation of water in hyaline cartilage.

• DOI: 10.1007/s10439-013-0844-0
• 发表时间: 2013-11
• 期刊: ANNALS OF BIOMEDICAL ENGINEERING
• 影响因子: 3.8
• 作者: Padalkar, M. V.;Spencer, R. G.;Pleshko, N.
• 通讯作者: Pleshko, N.

Wavelength-dependent penetration depth of near infrared radiation into cartilage.

• DOI: 10.1039/c4an01987c
• 发表时间: 2015-04-07
• 期刊: The Analyst
• 作者: Padalkar MV;Pleshko N
• 通讯作者: Pleshko N

Monitoring the Progression of Spontaneous Articular Cartilage Healing with Infrared Spectroscopy.

通过红外光谱监测自发关节软骨愈合的进展。

• DOI: 10.1177/1947603515572874
• 发表时间: 2015-07
• 期刊: Cartilage
• 影响因子: 2.8
• 作者: O'Brien MP;Penmatsa M;Palukuru U;West P;Yang X;Bostrom MP;Freeman T;Pleshko N
• 通讯作者: Pleshko N