Evaluation of Cartilage Tissue Engineering Strategies by IR Imaging

通过红外成像评估软骨组织工程策略

• 批准号: 9402967
• 负责人: Nancy Pleshko
• 金额: $ 5.7万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9402967
• 关键词: Address; Alpha Cell; Animal Model; Area; Biochemical; Biological Models; Biomechanics; Biopsy; Cartilage; Cell Count; Chondrocytes; Clinical; Clinical assessments; Collagen; Data; Defect; Development; Engineering; Evaluation; Exhibits; Family suidae; Fiber Optics; Functional disorder; Funding; Growth Factor; Harvest; Histologic; Human; Hyaluronic Acid; Image; Implant; In Vitro; Individual; Least-Squares Analysis; Magnetic Resonance Imaging; Mechanics; Methods; Miniature Swine; Modality; Modeling; Modulus; Molecular; Molecular Profiling; Molecular Structure; Monitor; Musculoskeletal; Near-Infrared Spectroscopy; Operative Surgical Procedures; Optical Methods; Outcome; Play; Production; Property; Proteoglycan; Protocols documentation; Regenerative Medicine; Role; Series; Spectroscopy, Fourier Transform Infrared; Techniques; Technology; Testing; Therapeutic; Thick; Time; Tissue Engineering; Tissues; Translating; Translations; Water; Work; X-Ray Computed Tomography; base; cartilage repair; cartilaginous; cell type; clinical development; clinical predictors; clinically relevant; design; implantation; in vivo; laboratory experiment; mechanical properties; microCT; mimetics; minimally invasive; novel; preimplantation; repaired; scaffold; subchondral bone; success; technique development; tissue regeneration; tissue repair

Summary: In spite of progress in understanding the pathophysiology of OA, there remain no definitive non-surgical treatment options. Further, tissue engineering strategies designed to produce cartilage-mimetic materials have seen only limited success; a significant impediment is the inability to fully assess the molecular structure of developing tissue such that optimal constructs for implantation can be identified. Recently, we demonstrated that the technique of non-destructive near infrared spectroscopy (NIRS) analysis can be utilized to assess the primary molecular components of native and engineered cartilage. NIRS data correlates strongly to water and matrix content (collagen and proteoglycan) in these tissues, and to mechanical properties. Having validated the NIR technology for cartilage assessment, we propose to extend our studies to address an important emerging area of musculoskeletal tissue engineering, identification of individual constructs suitable for clinical implantation. These studies are based on the principle that increases in construct maturation prior to implantation can be utilized to predict in vivo success. We hypothesize that maturation of the cartilaginous matrix can be monitored by NIRS, and that the spectral data can be used as a predictor of clinical success. We will address this in a series of in vitro and in vivo studies that evaluate construct development using NIRS analysis. A cell-seeded hyaluronic acid (HA) scaffold, previously shown to be effective at promoting matrix production and the development of functional material properties, will serve as the model system for implantation into a porcine model of cartilage repair. The proposed NIRS technology would establish a novel, non-destructive modality for pre-implantation evaluation of developing engineered tissues for clinical implantation. There are currently no minimally-invasive methods available for the clinical assessment of cartilage repair tissue maturation based on molecular signatures. The development of clinically-relevant optical methods to evaluate full-depth composition of tissues in a large animal model will provide a strong underpinning for translation of this technique into the clinical setting. Such analyses would also represent a fundamentally new and powerful approach for evaluating and guiding cartilage therapeutics. The possibility of one non-destructive modality that can be used in vitro during laboratory experiments of developing tissues to assess composition and identify constructs for implantation, as well as after the constructs are implanted clinically, would indeed be a powerful technique that could play a central role in the regenerative medicine field.

总结： 尽管在理解OA的病理生理学方面取得了进展，但仍然没有明确的非手术治疗方法。 治疗方案。此外，设计用于产生软骨模拟材料的组织工程策略已经 只看到有限的成功;一个重大的障碍是无法充分评估的分子结构 发育组织，从而可以鉴定用于植入的最佳构建体。最近，我们展示了 非破坏性近红外光谱（NIRS）分析技术可用于评估 天然和工程软骨的主要分子成分。近红外光谱数据与水和 这些组织中的基质含量（胶原蛋白和蛋白聚糖）以及机械性能。在确认了 近红外技术的软骨评估，我们建议扩大我们的研究，以解决一个重要的新兴 肌肉骨骼组织工程领域，适合临床的个体构建物的鉴定 置入这些研究是基于这样的原则，即在构建体成熟之前， 植入可用于预测体内成功。我们假设软骨的成熟 基质可以通过NIRS监测，并且光谱数据可以用作临床成功的预测因子。我们 将在一系列体外和体内研究中解决这一问题，这些研究使用NIRS评估构建体的发展 分析.一种细胞接种的透明质酸（HA）支架，以前被证明是有效的促进基质 生产和功能材料性能的发展，将作为模型系统， 植入到软骨修复的猪模型中。 提出的近红外光谱技术将建立一种新的，非破坏性的模式，为植入前 评价开发用于临床植入的工程化组织。目前还没有微创的 可用于基于分子生物学的软骨修复组织成熟的临床评估的方法 签名.临床相关光学方法的发展，以评估组织的全深度组成 在大型动物模型中的应用将为该技术转化为临床提供强有力的基础， 设置.这种分析也将是一种全新的、强有力的方法， 指导软骨治疗。一种非破坏性方式的可能性，可以在体外使用， 组织发育实验室实验，以评估组成并鉴定用于植入的结构，如 以及在临床上植入结构后，确实是一种强大的技术，可以发挥 在再生医学领域的核心作用。