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Resistance and Virulence of Surviving Bacteria in Water after Various Disinfection Processes

各种消毒过程后水中存活细菌的耐药性和毒力

基本信息

批准号：
9974535
负责人：
Yanyan Zhang
金额：
$ 14.8万
依托单位：
NEW MEXICO STATE UNIVERSITY LAS CRUCES
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-13 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9974535
关键词：
Address Aftercare Antibiotic Resistance Antibiotics Antimicrobial susceptibility Applications Grants Aquaculture Bacteria Bacterial Adhesins Bacterial Antibiotic Resistance Bacteriophages Characteristics Chlorine Databases Detection Development Disinfectants Disinfection Drug Metabolic Detoxication Effectiveness Environment Escherichia coli Excision Food Industry Foundations Frequencies Future Genes Genetic Transcription Goals Grant Health Horizontal Gene Transfer Human Immune system Individual Irrigation Knowledge Livestock Lytic Medical Device Metabolism Metagenomics Microbial Biofilms Modeling Organism Oxidative Stress Pathogenicity Phenotype Plants Plasmids Population Predisposition Process Production Property Prophage Inductions Pseudomonas aeruginosa Public Health Research Research Project Grants Resistance Risk Role SOS Response Safety Source Southwestern United States Structure System Taxonomy Testing Thick Toxin Variant Virulence Virulence Factors Water Water Purification Work bacterial community bacterial resistance biological adaptation to stress density microbial microbial community microorganism microorganism growth novel pathogen pathogenic bacteria programs resistance gene study characteristics water sampling water treatment

项目摘要

Project Summary: Bacterial pathogens in water pose a large world-wide threat to public health, especially for the population with weak immune system. While current disinfection strategies aim at mitigating the presence of pathogens, they do not completely eradicate the growth of microorganisms. This raises a question as to what happens to the surviving microorganisms after different disinfection processes. It is still unclear that if these surviving microorganisms are the same, less or more hazardous than the ones before disinfection. The answer to that question has a significant impact on microbial safety. As regards effective disinfection, what really matters is reduction of pathogenicity instead of the concentration of microorganisms. The long-term goal is to gain a better understanding on pathogenicity and susceptibility to antibiotics of surviving microorganisms after disinfection and elucidate the role of regulatory defense systems of surviving microorganisms. The proposed research project seeks to reveal the characteristic variations of bacteria before and after different disinfection processes by conducting systematic study to provide guidance regarding disinfection strategies. The primary objectives of this proposal will be achieved by addressing the following specific aims: Specific Aim 1. Investigate microbial community structure and functions in raw and treated water after various disinfections. Specific Aim 2. Determine the virulence and antibiotic resistance of surviving bacteria after disinfections. Specific Aim 3. Elucidate the role of disinfectants in horizontal gene transfer of antibiotic resistance genes and virulence factors. Achieving the above aims will provide fundamental knowledge on the characteristics of surviving bacteria after disinfections which is vital to public health. The enhanced knowledge will facilitate the development of novel disinfection strategies to reduce the pathogenicity of surviving bacteria and minimize the spread of bacteria with virulence and antibiotic resistance into water. These studies will also benefit the future R01 grant, where we will explore the correlations between disinfectant strategies and pathogenicity of surviving microorganisms and elucidate the role of regulatory defense systems of surviving microorganisms.

项目概述：水中的细菌病原体对公众健康构成了巨大的全球性威胁，特别是对于免疫系统较弱的人群。虽然目前的消毒策略旨在在减少病原体的存在，他们不完全根除的增长，微生物的这就提出了一个问题，即在微生物被感染后，不同的消毒过程。目前还不清楚这些存活的微生物是否是相同，比消毒前更少或更危险。这个问题的答案对微生物安全性有重大影响。至于有效的消毒，真正重要的是降低致病性而不是微生物的浓度。远景目标是为了更好地了解生存的病原性和对抗生素的敏感性，微生物消毒后，并阐明调控防御系统的作用，存活的微生物拟议的研究项目旨在揭示采用系统的方法，研究提供有关消毒策略的指引。本项目的主要目标该提案将通过解决以下具体目标来实现：具体目标1。探讨微生物群落结构和功能的原水和处理后的各种消毒。具体目标2。确定存活细菌的毒力和抗生素耐药性，消毒具体目标3。阐明消毒剂在水平基因转移中的作用抗生素抗性基因和毒力因子。实现上述目标将提供关于消毒后存活细菌特性的基本知识，对公共卫生至关重要。知识的提高将促进新型消毒剂的开发降低存活细菌的致病性和最大限度地减少细菌传播的策略并对抗生素产生抗药性。这些研究也将有利于未来的R01 格兰特，在那里我们将探讨消毒策略和致病性之间的相关性，存活的微生物并阐明存活的调节防御系统的作用微生物的