In Vivo Animal and Human Studies Core

体内动物和人类研究核心

• 批准号: 9978789
• 负责人: CHUNG OWYANG
• 金额: $ 16.55万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978789
• 关键词: 3-Dimensional; Acute; Animal Model; Animals; Basic Science; Biopsy; Cell Separation; Cellular biology; Chronic; Clinical; Clinical Research; Consultations; Data; Development; Digestive System Disorders; Disease; Education; Electroporation; Endoscopy; Ensure; Epithelial Cells; Equipment; Experimental Designs; Feedback; Funding; Future; Gastrointestinal tract structure; Growth; Health Services Accessibility; Homeostasis; Human; Human Resources; In Vitro; Individual; Infrastructure; Intervention; Investigation; Liquid substance; Measurement; Michigan; Molecular; Mucous Membrane; Mus; Online Systems; Organ; Organoids; Peer Review; Physiological; Preparation; Publications; Research; Research Design; Research Personnel; Resources; Sample Size; Sampling; Services; Small Interfering RNA; Specimen; Statistical Data Interpretation; Techniques; Technology; Time; Tissue Banks; Tissues; Translating; Translations; Universities; base; biobank; body system; cell dimension; cellular imaging; clinical application; data management; design; gastrointestinal; human model; human pluripotent stem cell; imaging study; in vitro Model; in vivo; induced pluripotent stem cell; instrument; member; molecular imaging; new technology; organizational structure; power analysis; programs; repository; statistics; therapeutic target; user-friendly

The objective of the In Vivo Animal and Human Studies core is to provide a venue for focusing human resources, facilities, new technology and equipment necessary for the in vivo study of humans and animals. The core serves to fully integrate investigations at the molecular, cellular, and organ system levels that are currently being performed within other cores of the Center and enhance rapid translation of basic discoveries into clinical application. The major services provided by the In Vivo Animal and Human Studies core are as follows:1. To provide a variety of acute and chronic animal models as well as state-of-the-art in vivo technologies to enhance rapid translation of basic discoveries to physiological and clinical applications; 2. To develop an organoid and enteroid program in which these preparations grown from mucosa biopsies and induced pluripotent stem cells (iPSCs) may be used to study epithelial cell biology and provide therapeutic targets for clinical intervention; 3. To support a clinical study program which will provide consultation and technical support to Center investigators on design and statistical data analysis; and 4. To provide a user friendly, one-step service to access digestive disease-related biospecimens for Center members. Since its inception in 1984, the In Vivo Animal and Human Studies core has evolved to meet the needs of Center investigators. Over time, older techniques which are readily available in clinical facilities have been discontinued by the Center. We have streamlined our animal studies to include ones that are frequently utilized by Center investigators. These include mice endoscopy, in vivo cellular and molecular imaging studies in small animals, fluorescent-activated cell sorting (FACS) analysis, and electroporation for direct transfer of siRNA into targeted neuro-tissue or GI organs. The recent development of the Enteroid and Organoid core enables Center investigators to conduct research on in vitro models of human iPSCs, directed differentiation of human pluripotent stem cells (hPSCs), and 3-dimensional growth of primary human and mouse tissue delivered from the GI tract. These preparations may be used to study human GI development, homeostasis and disease in vitro. To enhance the rapid translation of basic science findings to clinical applications, the In Vivo Studies core has spent much of its efforts and resources to upgrade its infrastructure. The Clinical Design Program provides consultation regarding clinical study design, subject specification and sampling techniques, measurements and instruments, sample size estimation, power analysis, as well as data management and statistical analysis. The Biospecimen Banking service will provide support to enhance existing individual GI- related biobanks and develop future repositories. This service also centralizes, expedites, and facilitates access to GI-related biobanks and utilization of GI biospecimens to translate basic research within the paradigm of human specimens. During this funding period, the In Vivo Animal and Human Studies core has been used by 60% of its Center members, has supported 166 publications, with 55 being collaborative.

活体动物和人类研究核心的目标是提供一个场所，让人类 人和动物活体研究所需的资源、设施、新技术和设备。 核心服务于完全整合分子、细胞和器官系统级别的研究，这些研究是 目前正在中心的其他核心内执行，并加强基本发现的快速转化 进入临床应用。In Vivo动物和人类研究核心提供的主要服务包括 1.提供多种急、慢性动物模型及体内最先进的动物模型 加强将基本发现快速转化为生理和临床应用的技术； 制定有机和肠类计划，其中这些准备工作是从粘膜活检和 诱导多能干细胞(IPSCs)可用于研究上皮细胞生物学和提供治疗 临床干预目标；3.支持一项临床研究计划，该计划将提供咨询和 向中心调查人员提供设计和统计数据分析方面的技术支持；以及4.为用户提供 友好的一步式服务，为中心成员提供与消化疾病相关的生物检疫。因为它的 成立于1984年，体内动物和人类研究核心已演变为满足中心的需求 调查人员。随着时间的推移，临床设施中容易获得的较老的技术已经 由本中心停产。我们已经简化了我们的动物研究，包括那些经常 由中心调查人员使用。这些研究包括小鼠内窥镜检查、体内细胞和分子成像研究。 在小动物中，荧光激活细胞分选(FACS)分析和电穿孔直接转移 SiRNA进入目标神经组织或胃肠道器官。肠核和器官核的最新研究进展 使中心研究人员能够对人IPSCs的体外模型进行研究，定向分化 人多能干细胞(HPSCs)与人和鼠原代组织的三维生长 从胃肠道送来的。这些制剂可用于研究人类胃肠道发育、动态平衡 和体外疾病。为加强基础科学研究成果迅速转化为临床应用， VIVO研究核心已经花费了大量的努力和资源来升级其基础设施。临床设计 该计划提供有关临床研究设计、受试者规格和抽样技术的咨询， 测量和仪器、样本量估计、功率分析以及数据管理和 统计分析。Biosecimen银行服务将提供支持，以增强现有的个人GI- 相关的生物库和开发未来的储存库。该服务还可以集中、加速和促进 获取与GI相关的生物库和利用GI生物样本将基础研究转化为 人类标本的范例。在这一资助期间，体内动物和人类研究核心有 该中心60%的成员都在使用，支持了166种出版物，其中55种是合作出版物。