Determining the levels of resistance and patterns of cross-resistance for common kdr alleles in Aedes aegypti

确定埃及伊蚊常见 kdr 等位基因的耐药水平和交叉耐药模式

• 批准号: 9978470
• 负责人: Jeffrey G Scott
• 金额: $ 26.62万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-25 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978470
• 关键词: Adult; Aedes; Affect; Alleles; Culicidae; Data; Dengue; Evolution; Goals; Individual; Insecticide Resistance; Insecticides; Measures; Monitor; Mutation; Pattern; Phenotype; Population; Population Genetics; Process; Recommendation; Research; Resistance; Resistance development; Sodium Channel; ZIKA; congenic; design; disorder control; genetic resistance; human disease; improved; programs; pyrethroid; resistance allele; resistance mechanism; vector; vector control; vector mosquito; voltage

SUMMARY/ABSTRACT: Here we seek to understand the evolution of resistance to pyrethroid insecticides in Aedes aegypti, an important vector of human diseases such as dengue and Zika. Pyrethroid insecticides are widely used for control of adult Aedes, but resistance has evolved in many populations, representing global challenge for control of this vector and the diseases it transmits. Finding strategies for delaying the development of resistance by mosquito vectors to the few available insecticides is critical. To do so, it is essential to identify the level of resistance conferred, by common resistance alleles, to insecticides that target the voltage sensitive sodium channel (VSSC). This information will fill a critical data gap in our understanding of the evolution of resistance and will inform what options exist in populations where the different resistance alleles are found. Our long-term goals are to understand the importance of different Vssc alleles in the evolution of resistance so that this important process can be understood and the development of resistance can be delayed. Our short-term goal is to measure the level of resistance conferred by different Vssc mutations found in populations across the globe. To accomplish this goal we will pursue two specific aims. Aim 1. Isolate seven congenic (to the susceptible Liverpool strain) strains that have one of the common unstudied Vssc resistance alleles. Aim 2. Determine the level of resistance conferred by each Vssc allele to twenty insecticides that target the VSSC. This proposal fills an important data gap in our understanding of insecticide resistance in A. aegypti by quantitating the phenotype that the different alleles confer. In addition, our results will demonstrate what cross-resistance spectrum is conferred by each allele. This information will provide us with a vastly improved understanding of pyrethroid resistance in A. aegypti, and yield key information for resistance management in this important vector of human disease.

摘要/摘要： 在这里，我们试图了解埃及伊蚊对拟除虫菊酯杀虫剂的抗性的演变， 登革热和寨卡病毒等人类疾病的重要传播媒介。拟除虫菊酯类杀虫剂应用广泛 用于控制成年伊蚊，但许多人群已经产生了抗药性，代表了全球 控制这种媒介及其传播的疾病面临着挑战。寻找延迟的策略 蚊媒对少数可用杀虫剂产生抗药性至关重要。要做的事 因此，有必要确定常见抗性等位基因所赋予的抗性水平，以 针对电压敏感钠通道 (VSSC) 的杀虫剂。此信息将填写 我们对耐药性演变的理解存在关键数据差距，并将告知我们采取什么选择 存在于发现不同抗性等位基因的人群中。 我们的长期目标是了解不同 Vssc 等位基因在进化中的重要性 抵抗力，以便可以理解这一重要过程，并且可以了解抵抗力的发展 被延迟。 我们的短期目标是测量不同 Vssc 突变带来的耐药水平 在全球人群中发现。为了实现这一目标，我们将追求两个具体目标。 目标 1. 分离 7 个同源菌株（对于易感利物浦菌株），它们具有以下特征之一： 常见的未经研究的 Vssc 抗性等位基因。 目标 2. 确定每个 Vssc 等位基因对 20 种杀虫剂的抗性水平 以 VSSC 为目标。 该提议填补了我们了解埃及伊蚊杀虫剂抗性的重要数据空白 通过定量不同等位基因赋予的表型。此外，我们的结果将 展示每个等位基因赋予的交叉耐药谱。该信息将 使我们对埃及拟除虫菊酯抗性和产量有了极大的了解 这一人类疾病重要媒介的耐药性管理的关键信息。