Bacteriophage modulation of mosquito microbiota

蚊子微生物群的噬菌体调节

• 批准号: 9979027
• 负责人: George Dimopoulos
• 金额: $ 20.47万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9979027
• 关键词: Adult; Aedes; Affect; Agriculture; Anopheles Genus; Anopheles gambiae; Antibodies; Area; Bacteria; Bacteriophages; Biology; Breeding; Cells; Communities; Complex; Culicidae; Cytolysis; Data; Databases; Development; Diagnostic; Enterobacter; Eukaryotic Cell; Evolution; Excision; Fertility; Food Additives; Genome; Gnotobiotic; Horizontal Gene Transfer; Immunity; Individual; Infection; Larva; Life; Literature; Longevity; Microbe; Midgut; Modification; Mosquito Control; Mosquito-borne infectious disease; Partner in relationship; Play; Pseudomonas; Research; Role; Sampling; Serratia; Shapes; Source; Specificity; Taxonomy; Techniques; Time; TimeLine; Vectorial capacity; Virus; Water; base; disorder control; experimental study; gut bacteria; life history; member; microbial; microbiome; microbiota; novel; pathogen; permissiveness; preference; superinfection; tool; trait; vector; vector competence

SUMMARY Mosquito associated bacteria have a major impact on larval development, life span, vector competence and mating preferences. Multiple studies have deciphered the taxonomic diversity of bacteria associated with various mosquito species. It has been shown that altered microbiota affects multiple mosquito life traits. Despite these studies, the functional role of individual bacterial taxa and their interactions with each other in the mosquito gut remains unknown. To study the role of individual bacteria from a complex community, targeted modulation of the mosquito microbiota is desired. Bacteriophages provide a perfect tool for selective removal of targeted bacteria from microbiota. This proposal aims to utilize bacteriophages to selectively modulate the mosquito microbiota and study its effect of various mosquito life-history traits. Based on literature and our own studies, we have identified five bacterial taxa that are frequently associated with various mosquito species. In aim 1, we will isolate bacteriophages from various environmental sources capable of infecting selected mosquito gut bacteria. In our preliminary experiments we have already successfully isolated multiple bacteriophages capable of selectively infecting various mosquito gut bacteria. In aim 2, we will use isolated bacteriophages to selectively modulate the microbiota of adult Aedes and Anopheles mosquitoes. The effect of this altered microbiota on mosquito life span, fecundity and vector competence will be studied. In aim 3, we will use isolated bacteriophages to selectively modulate the microbiota in larval breeding water. We will evaluate the effect of altered microbiota on larval development, and vector competence, lifespan and fecundity of adult mosquitoes. Our preliminary data with gnotobiotic larvae has shown that addition of bacteriophages to larval breeding water can affect the larval development. This project will be the first ever use of bacteriophages in the mosquito microbiota research and has the potential to be developed into a novel technique to control mosquito borne diseases.

概括 蚊子相关细菌对幼虫发育、寿命、媒介能力有重大影响 和交配偏好。多项研究破译了相关细菌的分类多样性 与各种蚊子种类。研究表明，微生物群的改变会影响蚊子的多种生命特征。 尽管有这些研究，单个细菌类群的功能作用及其彼此之间的相互作用 蚊子的肠道仍然未知。为了研究复杂群落中单个细菌的作用， 需要有针对性地调节蚊子微生物群。噬菌体提供了一个完美的工具 从微生物群中选择性去除目标细菌。该提案旨在利用噬菌体 选择性调节蚊子微生物群并研究其对各种蚊子生活史特征的影响。 根据文献和我们自己的研究，我们确定了五种经常相关的细菌类群 与各种蚊子种类。在目标 1 中，我们将从各种环境中分离噬菌体 能够感染选定的蚊子肠道细菌的来源。在我们的初步实验中我们已经 成功分离出多种能够选择性感染多种蚊子肠道细菌的噬菌体。 在目标 2 中，我们将使用分离的噬菌体来选择性调节成年伊蚊的微生物群和 按蚊。这种改变的微生物群对蚊子寿命、繁殖力和媒介的影响 将研究能力。在目标 3 中，我们将使用分离的噬菌体来选择性调节 幼体繁殖水中的微生物群。我们将评估改变的微生物群对幼虫发育的影响， 以及成年蚊子的媒介能力、寿命和繁殖力。我们对无菌菌的初步数据 幼虫研究表明，在幼虫繁殖水中添加噬菌体会影响幼虫的发育。 该项目将是首次在蚊子微生物群研究中使用噬菌体，并具有 有望发展成为控制蚊媒疾病的新技术。