Role of the extracellular matrix during Wolffian/epididymal duct morphogenesis

细胞外基质在沃尔夫/附睾管形态发生过程中的作用

基本信息

  • 批准号:
    9980704
  • 负责人:
  • 金额:
    $ 33.51万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-08-01 至 2023-05-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY The epididymis provides a unique luminal fluid microenvironment that allows for sperm maturation and survival, and disruptions to this function lead to male infertility. Further, disruptions to epididymal function may also arise as a consequence of abnormal fetal development, although very little is known either of the process of Wolffian duct/epididymal development or of the nature and causes of congenital defects that lead to male infertility. The central hypothesis of this application is that elongation and coiling of the Wolffian duct is crucial for the function of the resulting epididymis, and that failure to elongate and coil leads to male infertility. The overall goal is two-fold, to examine: (a) the mechanisms by which cells move to elongate the duct and (b) to dissect the underlying cellular and molecular mechanisms that regulate those cell movements. Although we have evidence that epithelial cells move by intercalating, we will test the hypothesis that intercalation-type movements of the mesenchyme cells that surround the duct and the en masse movement of mesenchymal cells in the interstitium contribute to ductal elongation and coiling. A combination of genetically modified mice, advanced microscopy including confocal, second harmonic two-photon, and atomic force microscopy, in vitro organ culture and contemporary software analyses will be used to test the hypotheses outlined in the following three specific aims: (1) To test the hypothesis that the stiffness (modulus) of the ECM undergoes dynamic changes during Wolffian duct morphogenesis thereby providing a biomechanical environment that promotes epithelial and mesenchymal cell intercalation and en masse movement of mesenchymal cells within the interstitium. (2) To test the hypothesis that radial intercalation of mesenchymal cells and the en mass movements of mesenchymal cells within the interstitium are major drivers of Wolffian duct elongation and coiling. (3) To test the hypothesis that Ptk7 and Rac1 regulate radial intercalation and en masse movement of mesenchymal cells via regulating ECM biomechanical properties through changes in its deposition and assembly during Wolffian duct development, which in turn are important for male fertility. Therefore, this application focuses on the role of the ECM during Wolffian duct morphogenesis paying special attention to the importance of its assembly, distribution and stiffness, and how this is coordinated to regulate mediolateral and radial intercalation of epithelial and mesenchymal cells respectively, and the en masse movement of mesenchymal cells within the interstitium. Coordination of these events is critical for Wolffian/epididymal duct development, and therefore, male fertility. The anticipated outcomes of this study will not only have a major impact on an area of reproductive biology that has been poorly understood, but will also contribute to our understanding of the fundamental process of tubular morphogenesis. Specifically they will provide an understanding as to how the regulation of growth of the epididymis during development is important clinically.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Barry T. Hinton其他文献

Protein tyrosine kinase 7 regulates extracellular matrix integrity and mesenchymal intracellular RAC1 and myosin II activities during Wolffian duct morphogenesis
  • DOI:
    10.1016/j.ydbio.2018.03.011
  • 发表时间:
    2018-06-01
  • 期刊:
  • 影响因子:
  • 作者:
    Bingfang Xu;Sérgio A.A. Santos;Barry T. Hinton
  • 通讯作者:
    Barry T. Hinton
The testicular and epididymal luminal amino acid microenvironment in the rat.
  • DOI:
    10.1002/j.1939-4640.1990.tb00186.x
  • 发表时间:
    1990-11
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Barry T. Hinton
  • 通讯作者:
    Barry T. Hinton
The male antifertility agents alpha chlorohydrin, 5-thio-D-glucose, and 6-chloro-6-deoxy-D-glucose interfere with sugar transport across the epithelium of the rat caput epididymidis.
雄性抗生育剂α氯醇、5-硫代-D-葡萄糖和6-氯-6-脱氧-D-葡萄糖干扰糖穿过大鼠附睾上皮的转运。
  • DOI:
  • 发表时间:
    1983
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Barry T. Hinton;Herman Hernandez;Stuart S. Howards
  • 通讯作者:
    Stuart S. Howards
Rapport sur la 3ème Conférence Internationale sur l’Epididyme
  • DOI:
    10.1007/bf03034655
  • 发表时间:
    2002-12-01
  • 期刊:
  • 影响因子:
    2.000
  • 作者:
    Barry T. Hinton;Joël R. Drevet
  • 通讯作者:
    Joël R. Drevet
Rat testis and epididymis can transport [3H] 3-O-methyl-D-glucose, [3H] inositol and [3H] alpha-aminoisobutyric acid across its epithelia in vivo.
大鼠睾丸和附睾可在体内转运[3H]3-O-甲基-D-葡萄糖、[3H]肌醇和[3H]α-氨基异丁酸穿过其上皮细胞。
  • DOI:
  • 发表时间:
    1982
  • 期刊:
  • 影响因子:
    3.6
  • 作者:
    Barry T. Hinton;Stuart S. Howards
  • 通讯作者:
    Stuart S. Howards

Barry T. Hinton的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Barry T. Hinton', 18)}}的其他基金

Role of the extracellular matrix during Wolffian/epididymal duct morphogenesis
细胞外基质在沃尔夫/附睾管形态发生过程中的作用
  • 批准号:
    9751347
  • 财政年份:
    2018
  • 资助金额:
    $ 33.51万
  • 项目类别:
Role of the extracellular matrix during Wolffian/epididymal duct morphogenesis
细胞外基质在沃尔夫/附睾管形态发生过程中的作用
  • 批准号:
    10407029
  • 财政年份:
    2018
  • 资助金额:
    $ 33.51万
  • 项目类别:
Role of the extracellular matrix during Wolffian/epididymal duct morphogenesis
细胞外基质在沃尔夫/附睾管形态发生过程中的作用
  • 批准号:
    10172943
  • 财政年份:
    2018
  • 资助金额:
    $ 33.51万
  • 项目类别:
Regulation of Postnatal Epididymal Cell Proliferation
产后附睾细胞增殖的调节
  • 批准号:
    9023569
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Embryonic Development of the Mammalian Epididymis
哺乳动物附睾的胚胎发育
  • 批准号:
    8850712
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Embryonic Development of the Mammalian Epididymis
哺乳动物附睾的胚胎发育
  • 批准号:
    8292483
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Embryonic Development of the Mammalian Epididymis
哺乳动物附睾的胚胎发育
  • 批准号:
    8442925
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Regulation of Postnatal Epididymal Cell Proliferation
产后附睾细胞增殖的调节
  • 批准号:
    8425061
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Regulation of Postnatal Epididymal Cell Proliferation
产后附睾细胞增殖的调节
  • 批准号:
    8618910
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:
Regulation of Postnatal Epididymal Cell Proliferation
产后附睾细胞增殖的调节
  • 批准号:
    8236636
  • 财政年份:
    2012
  • 资助金额:
    $ 33.51万
  • 项目类别:

相似海外基金

Pushing the envelope: atomic force microscopy imaging of the bacterial outer membrane during growth and division
挑战极限:生长和分裂过程中细菌外膜的原子力显微镜成像
  • 批准号:
    BB/X007669/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Research Grant
Nanoscopic elucidation of dynamic behavior of RNA viral nucleocapsid proteins using high-speed atomic force microscopy (HS-AFM)
使用高速原子力显微镜 (HS-AFM) 纳米级阐明 RNA 病毒核衣壳蛋白的动态行为
  • 批准号:
    24K18449
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Unravelling dengue virus structural dynamics and conformational changes using high-speed atomic force microscopy
使用高速原子力显微镜揭示登革热病毒结构动力学和构象变化
  • 批准号:
    24K18450
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
State-of-the-art atomic force microscopy facilities for South Australia
南澳大利亚最先进的原子力显微镜设施
  • 批准号:
    LE240100129
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Linkage Infrastructure, Equipment and Facilities
Atomic scale reactivity of small islands of a bimetallic alloy on ceria to small molecules investigated by ultrahigh resolution atomic force microscopy
通过超高分辨率原子力显微镜研究二氧化铈上双金属合金小岛对小分子的原子尺度反应性
  • 批准号:
    24K01350
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Pushing the envelope: atomic force microscopy imaging of the bacterial outer membrane during growth and division
挑战极限:生长和分裂过程中细菌外膜的原子力显微镜成像
  • 批准号:
    BB/X00760X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Research Grant
A New Nano Tip Fabrication Technique for Atomic Force Microscopy
原子力显微镜的新型纳米尖端制造技术
  • 批准号:
    DP230100637
  • 财政年份:
    2023
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Discovery Projects
Magnetic imaging by the locally induced anomalous Nernst effect using atomic force microscopy
使用原子力显微镜通过局部诱发的异常能斯特效应进行磁成像
  • 批准号:
    23K04579
  • 财政年份:
    2023
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Characterization of super adhesive aerosols on the basis of individual particle analysis using atomic force microscopy
基于原子力显微镜单个颗粒分析的超粘性气溶胶表征
  • 批准号:
    22KJ1464
  • 财政年份:
    2023
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Using atomic force microscopy to explore the processes and re-organisations that occur during bacterial growth and division and how these are influenc
使用原子力显微镜探索细菌生长和分裂过程中发生的过程和重组以及它们如何影响细菌
  • 批准号:
    2887441
  • 财政年份:
    2023
  • 资助金额:
    $ 33.51万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了