Animal and Behavior Core

项目总结 缺氧缺血所致围产期脑损伤仍是智力和体力障碍的主要原因 世界各地的残疾人。啮齿动物模型的使用将有助于预防和治疗 婴儿的终生损害。动物和行为核心(核心B)将为低氧大鼠提供 四个综合项目的缺血(H/I)，既往炎症和无炎症。要保持以下各项的一致性 在实验中，Core B还将使用拟议的神经保护疗法来治疗幼崽，包括低温治疗。 为了便于数据解释，动物和行为核心还将提供原油的标准评级 组织病理学，以便于确定神经保护性干预的有效性。损伤的可变性是 众所周知，这样的排名经常被用来确定轻度损伤大鼠的神经保护效果 与严重受伤的大鼠分开。这将特别通知那些需要组织被 均质，如免疫印迹、脂筏分离和核磁共振，因此不包括组织学 信息。这些先验排名将被用来跟踪随着时间的推移受伤的一致性，以及 让我们对神经保护化合物的潜在功效有一个更广泛的了解。核心B将组织和维护 保存项目I-IV的样本，并将其提供给项目I-IV的实验室。这样的核心 这些活动旨在避免为试点实验生产新动物的需要，这些实验可能会出现 数据收集正在进行中。动物和行为核心将执行新的行为组合 分析以确定小脑内调节失调的程度以及小脑与大脑之间的相互联系 小脑和其他脑区。最后，收集和通报关于以下方面进展情况的数据 将通过实施内联网可访问的关系数据库来促进实验。此数据库 将由核心B人员进行维护。该核心还将在适当的时间收集组织后H/I 对内部关系数据库中的组织样本进行具体实验和跟踪使用情况。该计划的总体目标 动物和行为的核心是确保质量控制和跨项目整合，以最大限度地发挥潜力 用于检测由H/I和/或围产期全身炎症引起的分子和行为损害，以及 治疗干预的效果。本计划项目赠款的一个关键优点是使用相同的 产生缺氧、缺血的程序和治疗。这将使项目一至四能够得出结论。 根据从其他项目收集的数据，快速并在必要时修改实验方法。

PROJECT SUMMARY Perinatal brain injury induced by hypoxia ischemia remains a leading cause of intellectual and physical disability throughout the world. The use of rodent models will contribute to both prevention and treatment of life-long impairment in infants. The Animal and Behavior Core (Core B) will provide rats subjected to hypoxia ischemia (H/I) with and without prior inflammation for four integrated Projects. To maintain consistency across experiments, Core B will also treat pups with the proposed neuroprotective treatments, including hypothermia. To facilitate data interpretation, the Animals and Behavior Core will also provide standard ratings of crude histopathology to facilitate determination of efficacy of neuroprotective interventions. The variability of injury is well recognized, and such rankings are often used to determine neuroprotection effects in mildly injured rats separately from severely injured rats. This will particularly inform experiments that require tissue to be homogenized, such as western blotting, lipid raft isolation and NMR, and therefore do not include histological information. These a priori rankings will be used to track both the consistency of the injury over time, and also give us a broader picture of potential efficacy of neuroprotective compounds. Core B will organize and maintain banked samples from Projects I-IV, and make them available to the laboratories of Projects I-IV. Such Core activities are designed to circumvent the need to produce new animals for pilot experiments that may arise as data collection progresses. The Animals and Behavior Core will execute novel combinations of behavioral assays to determine the extent of dysregulation within the cerebellum and reciprocal connections between the cerebellum and other brain regions. Finally, data collection and communication concerning progress of experiments will be facilitated by implementation of an IntraNet accessible relational database. This database will be maintained by Core B personnel. This Core will also collect tissue at the appropriate time post H/I for specific experiments and track use of tissue samples in the intrarelational database. The overall goal of the Animal and Behavior Core is to assure quality control and cross-project integration to maximize the potential for detecting molecular and behavioral impairments caused by H/I and/or perinatal systemic inflammation, and the effects of therapeutic interventions. A critical strength of this Program Project Grant is the use of the same procedures to generate hypoxia ischemia and treatments. This will enable Projects I-IV to make conclusions quickly and revise experimental approaches if necessary based on data collected from other Projects.