Animal and Behavior Core

项目摘要 围产期缺氧缺血性脑损伤是导致智力和体力低下的主要原因 全世界的残疾人。啮齿动物模型的使用将有助于预防和治疗 婴儿的终身损伤。动物和行为核心（核心B）将提供缺氧大鼠 缺血（H/I），有和没有事先炎症的四个综合项目。保持一致性 在实验中，核心B也将用拟议的神经保护治疗方法治疗幼崽，包括低温。 为了便于数据解释，动物和行为核心还将提供原油的标准评级， 组织病理学以促进确定神经保护干预的功效。伤害的可变性是 公认的，这样的排名通常用于确定轻度损伤大鼠的神经保护作用 与严重受伤的老鼠分开。这将特别告知需要组织被 匀浆，如蛋白质印迹，脂筏分离和NMR，因此不包括组织学 信息.这些先验的排名将被用来跟踪随着时间的推移受伤的一致性， 让我们对神经保护化合物的潜在功效有更广泛的了解。核心B将组织和维护 项目一至四的库存样品，并提供给项目一至四的实验室。这样的核心 这些活动旨在避免为试点实验生产新动物的需要， 数据收集工作正在进行。动物和行为核心将执行新的行为组合， 测定以确定小脑内失调的程度以及小脑与小脑之间的相互连接。 小脑和其他大脑区域。最后，还需要收集和交流关于 实验将通过实施IntraNet可访问的关系数据库来促进。此数据库 将由核心B人员维护。该核心还将在H/I后的适当时间收集组织， 特定的实验和跟踪使用的组织样本在intrarelational数据库。的总体目标 动物和行为的核心是确保质量控制和跨项目整合，以最大限度地发挥潜力 用于检测由H/I和/或围产期全身性炎症引起的分子和行为损伤，以及 治疗干预的效果。该计划项目赠款的一个关键优势是使用相同的 产生缺氧缺血的程序和治疗。这将使项目I-IV能够得出结论 根据从其他项目中收集的数据，迅速修改实验方法。

PROJECT SUMMARY Perinatal brain injury induced by hypoxia ischemia remains a leading cause of intellectual and physical disability throughout the world. The use of rodent models will contribute to both prevention and treatment of life-long impairment in infants. The Animal and Behavior Core (Core B) will provide rats subjected to hypoxia ischemia (H/I) with and without prior inflammation for four integrated Projects. To maintain consistency across experiments, Core B will also treat pups with the proposed neuroprotective treatments, including hypothermia. To facilitate data interpretation, the Animals and Behavior Core will also provide standard ratings of crude histopathology to facilitate determination of efficacy of neuroprotective interventions. The variability of injury is well recognized, and such rankings are often used to determine neuroprotection effects in mildly injured rats separately from severely injured rats. This will particularly inform experiments that require tissue to be homogenized, such as western blotting, lipid raft isolation and NMR, and therefore do not include histological information. These a priori rankings will be used to track both the consistency of the injury over time, and also give us a broader picture of potential efficacy of neuroprotective compounds. Core B will organize and maintain banked samples from Projects I-IV, and make them available to the laboratories of Projects I-IV. Such Core activities are designed to circumvent the need to produce new animals for pilot experiments that may arise as data collection progresses. The Animals and Behavior Core will execute novel combinations of behavioral assays to determine the extent of dysregulation within the cerebellum and reciprocal connections between the cerebellum and other brain regions. Finally, data collection and communication concerning progress of experiments will be facilitated by implementation of an IntraNet accessible relational database. This database will be maintained by Core B personnel. This Core will also collect tissue at the appropriate time post H/I for specific experiments and track use of tissue samples in the intrarelational database. The overall goal of the Animal and Behavior Core is to assure quality control and cross-project integration to maximize the potential for detecting molecular and behavioral impairments caused by H/I and/or perinatal systemic inflammation, and the effects of therapeutic interventions. A critical strength of this Program Project Grant is the use of the same procedures to generate hypoxia ischemia and treatments. This will enable Projects I-IV to make conclusions quickly and revise experimental approaches if necessary based on data collected from other Projects.