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Genes Underlying Reproductive Behavior and Physiology

生殖行为和生理学背后的基因

基本信息

批准号：
9979923
负责人：
MICHELLE N ARBEITMAN
金额：
$ 29.82万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9979923
关键词：
Adult Affinity Chromatography Anatomy Behavior Behavioral Brain Cell Adhesion Molecules Complex Courtship Development Drosophila genus Drosophila melanogaster Family Female Funding Gene Expression Gene Proteins Genes Genetic Genetic Determinism Genomics Goals Human Immunoglobulin Domain Knowledge Mediating Mental disorders Modeling Modernization Molecular Molecular Genetics Morphology Nervous System Physiology Nervous system structure Neurons Neurosciences Research Organism Population Positioning Attribute Process Protein Isoforms Regulator Genes Reproductive Behavior Reproductive Physiology Ribosomes Role Sex Differences Specific qualifier value Specificity Structure Synapses System Testing To specify Transcript Translating behavioral phenotyping behavioral study cell type connectome dimorphism experimental study extracellular gain of function genomic tools in vivo insight loss of function male neural circuit neuron development sex sexual dimorphism transcription factor transcriptome sequencing

项目摘要

Project Summary/Abstract One of the major challenges in neuroscience research is to understand the function of the brain at the level of genes, gene regulatory networks, neurons, and circuit structure. This proposal seeks to understand Drosophila reproductive behaviors, a highly tractable model, given knowledge of the master regulatory transcription factors and neuroanatomical structures that underlie these behaviors. fruitless (fru) encodes a set of master regulatory transcription factors, which are necessary and largely sufficient for specifying the potential for innate male courtship behaviors. This proposal is built on our results that demonstrate that a family of cell adhesion molecules, called Dprs and DIPs, have important functions in specifying the potential for reproductive behavior downstream of fru. We will determine the roles for behavior of subsets of fru-expressing neurons that overlap with Dpr/DIP expression (fru P1∩Dpr/Dip), using genetic intersectional strategies. We will also determine how anatomical sexual dimorphism in fru P1 neurons arises by functionally examining the roles of Dpr/DIPs. This study will also elucidate the Dpr/DIP interactome within fru-expressing neurons. Using cell-type specific genomic tools, we will also molecularly classify fru P1∩Dpr/Dip-expressing subpopulations to elucidate the molecular underpinnings that give rise to different neuronal functions.

项目总结/摘要神经科学研究的主要挑战之一是在神经元水平上理解大脑的功能。基因、基因调控网络、神经元和电路结构。这项提议旨在了解果蝇生殖行为，一个高度易处理的模型，给予知识的主要调控转录因子和神经解剖学结构来解释这些行为fruitless（fru）编码一组主调节转录因子，这是必要的，在很大程度上足以指定先天男性的潜力，求偶行为这项建议是建立在我们的结果，表明一个家庭的细胞粘附，一种叫做DIPs和DIP的分子，在确定生殖行为的潜力方面具有重要的功能 Fru的下游。我们将确定重叠的表达fru的神经元子集的行为的作用与Dpr/DIP表达（fru P1 → Dpr/Dip），使用遗传交叉策略。我们还将确定如何通过对Dpr/DIP作用的功能研究，发现了fru P1神经元的解剖学性别二态性。这研究还将阐明表达fru的神经元内的Dpr/DIP相互作用组。使用细胞类型特异性利用基因组工具，我们还将对fru P1和Dpr/Dip表达亚群进行分子分类，以阐明这些分子基础产生了不同的神经元功能。