Chromatin and Behavior

染色质和行为

• 批准号: 9239094
• 负责人: MICHELLE N ARBEITMAN
• 金额: $ 27.28万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9239094
• 关键词: Address; Adult; Affect; Affinity Chromatography; Alpha Cell; Animals; Antibodies; Behavior; Behavior Disorders; Behavioral; Binding; Biological; Biological Metamorphosis; Biological Models; Biological Process; Cells; Chromatin; Complex; Courtship; DNA; DNA Binding; Defect; Development; Disease; Drosophila genus; Environment; Epigenetic Process; Female; Foundations; Gene Expression; Genes; Genetic; Genetic Determinism; Genetic Transcription; Genomics; Goals; HDAC1 gene; Health; Human; Individual; Intervention; Knowledge; Laboratories; Link; Maintenance; Mental Health; Messenger RNA; Modeling; Modernization; Modification; Molecular; Molecular Biology; Molecular Genetics; Mushroom Bodies; Nervous system structure; Neurons; Neurosciences Research; Nuclear; Nucleotides; Phenotype; Physiological; Plant Roots; Positioning Attribute; Process; Production; Protein Isoforms; Proteins; RNA; RNA Splicing; Reproductive Behavior; Resolution; Ribosomes; Role; Sex Characteristics; Shapes; Solid; Specific qualifier value; Structure; Synapses; System; Thinking; Time Factors; To specify; Translating; Variant; behavior influence; cell type; chromatin immunoprecipitation; chromatin modification; cofactor; conditioning; experience; experimental study; genome-wide; histone modification; in vivo; innovation; insight; long term memory; mRNA Precursor; male; molecular phenotype; nervous system disorder; neural circuit; novel; novel therapeutics; predictive modeling; sex; tool; transcription factor; transcriptome sequencing

Project Summary/Abstract A major goal of neuroscience research is to understand the molecular-genetic specification of behaviors and how the environment influences these mechanisms. Lack of knowledge of these molecular-genetic mechanisms is a major barrier to progress, as this limits knowledge about the interplay between nuclear, synaptic and physiological neuronal functions that direct behaviors and plasticity. Understanding the molecular- genetic mechanisms that drive complex behaviors in model systems is an important step. We propose a collaborative and unique approach to address our lack of understanding of complex behaviors. This study will be one of the first to examine on a genome-wide scale several molecular phenotypes that drive complex behaviors. We propose to use state-of-the-art tools, which are newly developed by our laboratory, to examine at a cell-specific level several molecular phenotypes. We will examine chromatin, transcription factor binding, gene expression and alternative pre-mRNA splicing in neural circuits that are well defined and known to underlie behavior. This innovative approach will allow us to identify the molecular process that are required to drive the potential and maintenance of this behavior, by examining these phenotypes during development and adult stages. In addition, we examine how the environment can modify behavior by examining the molecular and neural circuit basis of long-term memory formation. The project will be the first to elucidate and integrate, in a cell-specific manner, several molecular phenotypes that direct complex behavior, including how sex- differences in the molecular environment influence behavior. We will statistically integrate knowledge of all of these phenotypes to gain insights into the complex interplay between environment, molecular, neural circuit and behavioral phenotypes.

项目总结/摘要 神经科学研究的一个主要目标是了解行为的分子遗传规范， 环境如何影响这些机制。缺乏这些分子遗传学知识 机制是取得进展的主要障碍，因为这限制了对核武器之间相互作用的了解， 指导行为和可塑性的突触和生理神经元功能。了解分子- 在模型系统中驱动复杂行为的遗传机制是重要的一步。我们提出了一个 协作和独特的方法来解决我们对复杂行为的理解不足。本研究将 是第一个在全基因组范围内研究几种分子表型的人， 行为。我们建议使用我们实验室新开发的最先进的工具来检查 在细胞特异性水平上的几种分子表型。我们将检查染色质，转录因子结合， 在神经回路中的基因表达和选择性前mRNA剪接是明确定义的， 行为的基础这种创新的方法将使我们能够确定所需的分子过程， 通过在发育过程中检查这些表型，推动这种行为的潜力和维持， 成人阶段此外，我们还研究了环境如何通过检查分子改变行为。 以及长期记忆形成的神经回路基础。该项目将是第一个阐明和整合， 以细胞特异性的方式，几种指导复杂行为的分子表型，包括性- 分子环境的差异影响行为。我们将统计整合所有的知识， 这些表型，以深入了解环境，分子，神经回路之间的复杂相互作用， 和行为表型。